本文選題：變應(yīng)性鼻炎　切入點(diǎn)：甲基化　出處：《新疆醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：針對(duì)維吾爾族、漢族變應(yīng)性鼻炎患者和正常健康人IL-4、IFN-γ基因甲基化進(jìn)行研究，分別探討IL-4、IFN-γ基因甲基化在維吾爾族、漢族AR患者與健康者是否存在差異性。方法：選擇維吾爾族、漢族AR患者各50例，維吾爾族、漢族正常人各50例，提取外周血液DNA，，運(yùn)用甲基化特異性PCR(MSP)方法檢測(cè)IL-4、IFN-γ基因甲基化。結(jié)果：漢族AR病例組與正常對(duì)照組IL-4基因甲基化率的比較具有統(tǒng)計(jì)學(xué)意義(P0.05)。漢族與維族正常對(duì)照組之間IL-4基因甲基化率的比較有統(tǒng)計(jì)學(xué)意義(P0.05)。漢族與維族AR病例組IL-4基因甲基化率的比較，無統(tǒng)計(jì)學(xué)意義(P0.05)。維族AR病例組與正常對(duì)照組IL-4基因甲基化率的比較，無統(tǒng)計(jì)學(xué)意義(P0.05)。漢族、維族AR病例組以及AR病例組與正常對(duì)照IFN-γ基因甲基化率的比較，均無統(tǒng)計(jì)學(xué)意義(P0.05)，漢族與維族正常對(duì)照組IFN-γ基因甲基化率的比較有統(tǒng)計(jì)學(xué)意義(P0.05)，維、漢族變應(yīng)性鼻炎患者皮膚變應(yīng)原點(diǎn)刺實(shí)驗(yàn)中艾蒿和藜屬陽性率最高，維、漢族相比差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論：艾蒿和藜屬是維、漢族AR患者室外主要吸入性變應(yīng)原。維吾爾族與漢族AR病例組之間IL-4基因啟動(dòng)子區(qū)甲基化陽性結(jié)果無差異，但漢族病例組與漢族健康對(duì)照組陽性結(jié)果有差異，說明IL-4基因甲基化可能在變應(yīng)性鼻炎的發(fā)病過程中發(fā)揮著重要作用。漢族健康人群中IL-4基因半甲基化高于維吾爾族健康人群，說明漢族人群中的IL-4甲基化有可能趨向于陽性表達(dá)，漢族人群易患上變應(yīng)性鼻炎。維吾爾族與漢族AR病例組以及正常對(duì)照組之間IFN-γ啟動(dòng)子區(qū)甲基化陽性結(jié)果均無差異性，但漢族與維族正常對(duì)照組之間有統(tǒng)計(jì)學(xué)差異，IFN-γ基因甲基化在變應(yīng)性鼻炎作用有待于進(jìn)一步研究。
[Abstract]:Objective: to study the methylation of IFN- 緯 gene in Uygur, Han nationality patients with allergic rhinitis and normal controls, and to explore the methylation of IFN- 緯 gene in Uygur nationality. Methods: there were 50 cases of AR in Uygur nationality, 50 cases in Han nationality, 50 cases in Uygur nationality and 50 cases in normal Han nationality. The DNA of peripheral blood was extracted, and the methylation of IFN- 緯 gene of IL-4 was detected by methylation specific PCR- MSP.Results: the methylation rate of IL-4 gene in AR patients of Han nationality was significantly higher than that in normal controls (P 0.05). The normal controls of Han nationality and Uygur nationality were also used to detect the methylation of IL-4 IFN- 緯 gene. The comparison of methylation rate of IL-4 gene between Han and Uygur patients was statistically significant (P 0.05), and the comparison of IL-4 methylation rate between Han and Uygur AR patients, There was no significant difference in methylation rate of IL-4 gene between Uygur and normal controls. The methylation rate of IFN- 緯 gene in Han, Uygur and AR cases and normal controls were compared. The methylation rate of IFN- 緯 gene in Han nationality and Uygur group was significantly higher than that in Uygur group. The positive rates of Artemisia annua and Chenopodium were the highest in the skin allergen prick test of patients with allergic rhinitis in Han nationality. Conclusion: Artemisia Artemisia and Chenopodium are the main outdoor inhalants in AR patients of Han nationality. There is no difference in methylation of promoter region of IL-4 gene between Uygur and Han patients. However, the positive results of IL-4 gene methylation may play an important role in the pathogenesis of allergic rhinitis in the Han nationality and the Han healthy control group, and the semi-methylation of the IL-4 gene in the Han nationality healthy population is higher than that in the Uygur healthy population, which indicates that methylation of IL-4 gene may play an important role in the pathogenesis of allergic rhinitis. The results indicated that IL-4 methylation might tend to positive expression in Han population, and the Han population was susceptible to allergic rhinitis. There was no difference in methylation positive results between Uygur and Han patients with AR and normal controls. However, there were significant differences between the Han and Uygur normal controls. The role of IFN- 緯 gene methylation in allergic rhinitis needs to be further studied.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R765.21

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