本文關(guān)鍵詞： 角膜移植 青蒿素 排斥反應(yīng)　出處：《遼寧醫(yī)學(xué)院學(xué)報(bào)》2016年06期 　論文類型：期刊論文

【摘要】：目的探討全身給予青蒿素,研究其對大鼠角膜移植排斥反應(yīng)的抑制作用。方法本實(shí)驗(yàn)通過把Wistar大鼠和SD大鼠作為移植給予和接受的對象,建立角膜移植的動(dòng)物模型。隨機(jī)分為4組,正常組:不行角膜手術(shù),也不藥物干預(yù);陽性對照組:給予1%羧甲基纖維素鈉(CMC-Na)溶液1.5 m L;環(huán)孢霉素A組:給予環(huán)孢霉素A 10 mg/kg;青蒿素組:給予青蒿素600 mg/kg。術(shù)后在顯微鏡下觀察各組角膜植片的狀態(tài),記錄生存時(shí)間及排除反應(yīng)指數(shù),HE染色觀察角膜植片的病理變化。結(jié)果陽性對照組、環(huán)孢霉素A組、青蒿素組角膜植片的平均存活時(shí)間分別為(11.67±0.67)d、(20.50±0.76)d、(18.67±0.67)d,環(huán)孢霉素A組、青蒿素組與陽性對照組比較,角膜植片的存活時(shí)間都明顯增加。病理學(xué)檢查結(jié)果:對照組可見角膜水腫,厚度增加,有較多的炎癥細(xì)胞,周圍可見新生血管,環(huán)孢霉素A組、青蒿素組的角膜植片均較陽性對照組明顯好轉(zhuǎn),水腫程度減輕、有少量炎性細(xì)胞浸潤,效果明顯好于陽性對照組。結(jié)論全身給予青蒿素,可以使移植術(shù)后角膜植片存活的時(shí)間延長,使角膜的水減輕腫,減少炎細(xì)胞的浸潤,從而能夠抑制移植免疫排斥反應(yīng)的發(fā)生。
[Abstract]:Objective to investigate the inhibitory effect of artemisinin on corneal allograft rejection in rats. Methods Wistar rats and SD rats were used as recipients. Animal models of corneal transplantation were established. They were randomly divided into 4 groups: normal group: no corneal surgery, no drug intervention; The positive control group was given 1% CMC-Na solution, the cyclosporine A group was given cyclosporine A 10 mg / kg, and the artemisinin group was given artemisinin 600 mg / kg. Results the mean survival time of corneal grafts in the positive control group, cyclosporine A group and artemisinin group were 11.67 鹵0.67 d (20.50 鹵0.76) and 18.67 鹵0.67 days respectively, and those in cyclosporine A group were 18.67 鹵0.67 days. The survival time of corneal grafts in the artemisinin group was significantly longer than that in the positive control group. Pathological examination showed that corneal edema was observed in the control group, the thickness increased, there were more inflammatory cells, neovascularization was seen around, and cyclosporine A group. The corneal grafts in the artemisinin group were better than those in the positive control group, the degree of edema was reduced, and a small amount of inflammatory cells infiltrated, the effect was obviously better than that in the positive control group. It can prolong the survival time of corneal grafts after transplantation, reduce the swelling of cornea, reduce the infiltration of inflammatory cells, and inhibit the occurrence of allograft rejection.
【作者單位】： 錦州醫(yī)科大學(xué)附屬第一醫(yī)院;天津市武警后勤學(xué)院附屬醫(yī)院;錦州市中心醫(yī)院;
【基金】：遼寧省自然科學(xué)基金計(jì)劃項(xiàng)目,項(xiàng)目編號(hào):2013022001
【分類號(hào)】：R779.65
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本文編號(hào)：1542223