本文關(guān)鍵詞： 眼附屬器淋巴瘤 AEG-1 LEF-1 MALT　出處：《青島大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:研究AEG-1(astrocyte elevated gene-1)、LEF-1(即淋巴細(xì)胞增強(qiáng)因子)在眼附屬器粘膜相關(guān)組織淋巴瘤(marginal zone lymphoma of mucosa-associated lymphoid tissue type,MALT)和反應(yīng)性淋巴組織增生的表達(dá),從蛋白和基因水平探討AEG-1和LEF-1的表達(dá)與眼附屬器MALT淋巴瘤發(fā)生發(fā)展關(guān)系,其蛋白表達(dá)與臨床病理特征的關(guān)系和兩者蛋白表達(dá)的相互關(guān)系及可能的機(jī)制。方法:搜集1995年1月到2015年1月青島大學(xué)附屬醫(yī)院眼科病理實(shí)驗(yàn)室眼附屬器MALT淋巴瘤石蠟包埋標(biāo)本和凍存組織,其中40例石蠟標(biāo)本、8例凍存組織為實(shí)驗(yàn)組,對(duì)照組為反應(yīng)性淋巴組織增生,其中石蠟標(biāo)本20例、凍存組織8例。應(yīng)用免疫組織化學(xué)染色法檢測(cè)AEG-1和LEF-1蛋白水平的表達(dá),應(yīng)用實(shí)時(shí)熒光定量PCR法檢測(cè)實(shí)驗(yàn)組和對(duì)照組中AEG-1和LEF-1m RNA水平的表達(dá),統(tǒng)計(jì)學(xué)分析采用SPSS17.0。結(jié)果:1.AEG-1的蛋白表達(dá)及m RNA含量:a.AEG-1陽(yáng)性顆粒主要定位在細(xì)胞胞膜上,少量在胞漿內(nèi)(圖3)。b.眼附屬器MALT淋巴瘤中AEG-1的陽(yáng)性表達(dá)率(62.5%)顯著高于反應(yīng)性淋巴組織增生(P0.05,表1)。c.AEG-1的陽(yáng)性表達(dá)與眼附屬器MALT淋巴瘤的臨床分期有關(guān)(P0.05,表2),隨著臨床分期的增加,其表達(dá)增加,而與性別、年齡、腫瘤發(fā)生部位無(wú)關(guān)。d.AEG-1 m RNA在眼附屬器MALT淋巴瘤中顯著高于反應(yīng)性淋巴組織增生(P0.05,圖9)。2.LEF-1的蛋白表達(dá)及m RNA含量:a.LEF-1陽(yáng)性顆粒主要定位在細(xì)胞胞核內(nèi)(圖6)。b.眼附屬器MALT淋巴瘤中LEF-1的陽(yáng)性表達(dá)率(65%)顯著高于反應(yīng)性淋巴組織增生(P0.05,表3)。c.LEF-1的陽(yáng)性表達(dá)與眼附屬器MALT淋巴瘤的臨床分期有關(guān)(P0.05,表4),隨著臨床分期的增加,其表達(dá)增加,而與患者性別、年齡、腫瘤發(fā)生部位無(wú)關(guān)。d.AEG-1 m RNA在眼附屬器MALT淋巴瘤中顯著高于反應(yīng)性淋巴組織增生(P0.05,圖10)。3.AEG-1和LEF-1蛋白表達(dá)與眼附屬器MALT淋巴瘤的關(guān)系:AEG-1和LEF-1在眼附屬器MALT組織中表達(dá)增高,陽(yáng)性表達(dá)率分別為62.5%、65%,兩者之間存在正相關(guān)(P0.05,表5)Spearman相關(guān)系數(shù)為r=0.406。結(jié)論:AEG-1和LEF-1的表達(dá)增高可能參與眼附屬器MALT淋巴瘤的發(fā)生發(fā)展,兩者表達(dá)成正相關(guān),可能通過(guò)某種模式在眼附屬器MALT中發(fā)揮作用。兩種因子可能成為眼附屬器MALT淋巴瘤的診斷標(biāo)記物,為眼附屬器淋巴瘤病理診斷提供依據(jù)。
[Abstract]:Objective: to study the expression of AEG-1(astrocyte elevated gene-1 lef 1 (Lymphocyte Enhancement Factor) in peripheral zone lymphoma of mucosa-associated lymphoid tissue type malt and reactive lymphoid tissue hyperplasia. To explore the relationship between the expression of AEG-1 and LEF-1 and the occurrence and development of ocular accessory MALT lymphoma from the protein and gene levels. The relationship between protein expression and clinicopathological features, the relationship between protein expression and the possible mechanism were analyzed. Methods: from January 1995 to January 2015, MALT lymph nodes were collected in ophthalmic pathology laboratory of Qingdao University Hospital. Paraffin embedded specimens and frozen tissue, The expression of AEG-1 and LEF-1 protein was detected by immunohistochemical staining method in 40 paraffin specimens and 8 frozen tissues, including 20 paraffin wax specimens and 8 frozen tissues. The expression of AEG-1 and LEF-1m RNA in the experimental group and the control group were detected by real-time fluorescence quantitative PCR method. SPSS 17.0. the results showed that the protein expression of 1 / 1 AEG-1 and the content of m RNA were mainly located on the cell membrane. The positive expression rate of AEG-1 in cytoplasm (Fig. 3n.b. ocular accessory MALT lymphoma) was significantly higher than that in reactive lymphoid tissue hyperplasia (P0.05. Table 1. C. AEG-1 was associated with the clinical staging of MALT lymphoma of ocular appendage, P 0.05, Table 2, with the increase of clinical stage. Its expression increases with sex, age, There was no correlation between tumor location and tumor location. D. AEG-1 m RNA was significantly higher in ocular adnexal MALT lymphoma than in reactive lymphoid tissue hyperplasia (P0.05). Fig. 9. 2. The protein expression of LEF-1 and the content of m RNA:: a.LEF-1 were mainly located in the cellular nucleus (Fig. 6). The positive expression rate of LEF-1 in lymphoma was significantly higher than that in reactive lymphoproliferative tissue hyperplasia (P 0.05). The positive expression of LEF-1 was related to the clinical stage of ocular adnexal MALT lymphoma (P 0.05, Table 4), and with the increase of clinical stage, the positive expression of Lymphoma was significantly higher than that of reactive lymphoid tissue hyperplasia (P 0.05). Its expression increased with the patient's gender, age, The expression of .d.AEG-1 m RNA in ocular adnexal MALT lymphoma was significantly higher than that in reactive lymphoid tissue hyperplasia (P0.05). Fig. 10. 3. The relationship between the expression of AEG-1, AEG-1 and LEF-1 protein and the expression of LEF-1 in ocular adnexal MALT lymphoma was significantly higher than that in ocular adnexal MALT lymphoma. The positive expression rates were 62.5% and 65%, respectively. There was a positive correlation between them (P 0.05), and the correlation coefficient between them was 0.406.Conclusion the increased expression of LEF-1 and AEG-1 may be involved in the occurrence and development of ocular appendage MALT lymphoma, and there is a positive correlation between them. These two factors may be used as diagnostic markers of ocular accessory MALT lymphoma and provide basis for pathological diagnosis of ocular adnexal lymphoma.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R739.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王芬;林紅;趙桂秋;林靜;胡麗婷;魯軍霞;;眼附屬器MALT淋巴瘤Survivin及Caspase-3表達(dá)及意義[J];齊魯醫(yī)學(xué)雜志;2016年03期

2 金哈斯;國(guó)文;趙秀芳;;23例原發(fā)眼附屬器淋巴瘤臨床特點(diǎn)及治療分析[J];武警后勤學(xué)院學(xué)報(bào)(醫(yī)學(xué)版);2016年01期

3 魯軍霞;林紅;王芬;林靜;張麗娜;趙桂秋;;Livin及Caspase-3在眼附屬器淋巴組織增生病變中的表達(dá)[J];國(guó)際眼科雜志;2015年12期

4 何小金;邢莉;;眼附屬器淋巴造血組織增生性疾病臨床病理分析[J];首都醫(yī)科大學(xué)學(xué)報(bào);2014年06期

5 秦瑞英;楊君;秦海霞;王宏偉;任艷芳;王慧玲;;siRNA下調(diào)AEG-1表達(dá)對(duì)乳腺癌細(xì)胞增殖和凋亡的影響[J];中國(guó)婦幼保健;2014年19期

6 黃美金;吳雪銘;王春芳;張富億;;AEG-1基因在非小細(xì)胞肺癌中的表達(dá)及其意義[J];重慶醫(yī)學(xué);2014年05期

7 郭衛(wèi)東;張惠潔;牛德森;屈振杰;;β-catenin、LEF-1和PTEN在乳腺癌中的表達(dá)及意義[J];臨床腫瘤學(xué)雜志;2013年12期

8 王文娟;南克俊;王淑紅;胡婷華;姜麗麗;馬婕群;;LEF1和Jagged1在人結(jié)直腸癌組織中的表達(dá)及其意義[J];西安交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2013年04期

9 任坤;閆康;張迎龍;曾照輝;劉濤;裘秀春;馬保安;;siRNA干擾LEF-1的表達(dá)對(duì)骨肉瘤細(xì)胞株F5M2侵襲和遷移的影響[J];現(xiàn)代腫瘤醫(yī)學(xué);2013年03期

10 李玉珍;蔡季平;徐放;李由;魏銳利;;眼附屬器MALT淋巴瘤的臨床特征及復(fù)發(fā)因素分析[J];中國(guó)實(shí)用眼科雜志;2012年10期

，

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