本文關(guān)鍵詞： 眼眶淋巴瘤 組織芯片 免疫組織化學染色 P53BCL-2Survivin 預(yù)后　出處：《天津醫(yī)科大學》2012年碩士論文　論文類型：學位論文

【摘要】：研究目的 運用組織芯片的相關(guān)技術(shù)分析眼眶淋巴瘤中凋亡相關(guān)基因Survivin、P53、 BCL-2的表達與其惡性程度和遠期生存率的關(guān)系。 研究方法 首先收集46例眼眶非霍奇金淋巴瘤患者及17例眼眶淋巴組織增生患者組織標本蠟塊；設(shè)46例眼眶淋巴瘤患者為實驗組,17例眼眶淋巴組織增生患者為對照組,其中實驗組再根據(jù)年齡、性別、病程、腫瘤大小及預(yù)后分組。同時搜集該63名患者的臨床資料信息,并隨訪該46名眼眶淋巴瘤患者的預(yù)后情況； 其次制作淋巴瘤及淋巴組織增生的組織芯片,并對組織芯片進行免疫組化染色；鏡下分析實驗組及對照組Survivin、P53、BCL-2表達的陽性率； 最后應(yīng)用SPSS17.0統(tǒng)計學軟件對5、6兩者的數(shù)據(jù)結(jié)果進行統(tǒng)計分析,以α=0.05為檢驗水準,P0.05認為有統(tǒng)計學意義。 研究結(jié)果 1、46例眼眶淋巴瘤和17例眼眶反應(yīng)性淋巴增生組織芯片經(jīng)HE染色后,病理診斷結(jié)果與原取材的大組織診斷完全一致。 2、三種凋亡相關(guān)蛋白,Bcl-2和P53在眼眶淋巴瘤和反應(yīng)性淋巴組織增生的組織芯片中均有不同程度表達,Survivin在兩種組織中均為陰性表達。Bcl-2蛋白主要表達于細胞膜,在兩種病變的陽性表達率為84.8%和58.8%,其差異有統(tǒng)計學意義(P0.05)；P53表達于細胞漿,在兩種病變的陽性表達率為82.6%和52.9%,其差異有統(tǒng)計學意義(P0.05)。 3、46例眼眶淋巴瘤在預(yù)后不良組和預(yù)后良好組中,Bcl-2在兩組間的表達率分別為96.4%和66.7%,其差異有統(tǒng)計學意義(P0.05)；P53在兩組間的表達率分別為92.9%和66.7%,其差異有統(tǒng)計學意義(P0.05)。 4、46例淋巴瘤：Bcl-2和P53這兩種蛋白的陽性表達率在≥60歲和60歲年齡組間、男女性別組間、≥2.5cm和2.5cm腫瘤組間及≥1年和1年發(fā)病時間組間均無統(tǒng)計學意義。 5、Bcl-2和P53在眼眶淋巴瘤中的表達呈正相關(guān)(P0.05)。 結(jié)論 1、組織芯片技術(shù)高效、經(jīng)濟、快速、準確地研究了眼眶淋巴瘤的凋亡相關(guān)基因表達情況。 2、Bcl-2在眼眶淋巴瘤和反應(yīng)性淋巴組織增生之間陽性率表達的差異有統(tǒng)計學意義,說明Bcl-2對判斷眼眶淋巴細胞性疾病的良惡性有一定價值；同時Bcl-2在眼眶淋巴瘤的預(yù)后不良組和預(yù)后良好組之間陽性率表達差異有統(tǒng)計學意義,說明Bcl-2可以預(yù)測眼眶淋巴瘤預(yù)后。 3、P53在眼眶淋巴瘤和反應(yīng)性淋巴組織增生之間陽性率表達的差異有統(tǒng)計學意義,說明P53對判斷眼眶淋巴細胞性疾病的良惡性有一定價值；同時P53在眼眶淋巴瘤的預(yù)后不良組和預(yù)后良好組之間陽性率表達差異有統(tǒng)計學意義,說明P53可以預(yù)測眼眶淋巴瘤預(yù)后。 4、Survivin在眼眶淋巴瘤和反應(yīng)性淋巴組織增生組中均呈陰性表達,提示Survivin與眼眶淋巴細胞性疾病的病變性質(zhì)及預(yù)后無明確相關(guān)性。 5、Bcl-2和P53蛋白在眼眶淋巴瘤中的表達呈正相關(guān)性,提示聯(lián)合檢測這兩種指標對預(yù)測眼眶淋巴瘤的預(yù)后有較大價值。
[Abstract]:Research purpose. The expression of apoptosis-related genes survivin p53 and BCL-2 in orbital lymphoma was analyzed by tissue microarray technique. The expression of survivin p53 and BCL-2 was correlated with the degree of malignancy and the long-term survival rate. Research method. At first, 46 cases of orbital non-Hodgkin 's lymphoma and 17 cases of orbital lymphoid tissue hyperplasia were collected, and 46 cases of orbital lymphoma were taken as experimental group and 17 cases of orbital lymphoid tissue hyperplasia as control group. The experimental group was grouped according to age, sex, course of disease, tumor size and prognosis. The clinical data of 63 patients were collected and the prognosis of 46 patients with orbital lymphoma was followed up. Then, the tissue microarray of lymphoma and lymphoid tissue proliferation was made, and the immunohistochemical staining of tissue microarray was carried out, and the positive rate of survivin P53 BCL-2 expression in experimental group and control group was analyzed under microscope. At last, the statistical analysis of the data of the two groups was carried out by using the SPSS17.0 statistical software, and the statistical significance was found to be statistically significant with 偽 0. 05 as the test level (P0.05). Research results. 1the pathological diagnosis of 46 cases of orbital lymphoma and 17 cases of orbital reactive lymphoid hyperplasia by HE staining was completely consistent with that of the original large tissue. 2, three kinds of apoptosis-related proteins Bcl-2 and p53 were expressed in orbital lymphoma and reactive lymphoproliferative tissue microarray to varying degrees. The expression of survivin was negative in both tissues. Bcl-2 protein was mainly expressed on cell membrane. The positive expression rates of p53 were 84.8% and 58.8 in the two kinds of lesions, and the difference was statistically significant. The positive expression rates of p53 in the cytoplasm were 82.6% and 52.9 in the two kinds of lesions, respectively. The difference was statistically significant (P 0.05). 3The expression rate of Bcl-2 was 96.4% and 66.7 in the poor prognosis group and the good prognosis group, respectively, and the difference was statistically significant. The expression rate of P0.05 and p53 in the two groups was 92.9% and 66.7, respectively. The difference was statistically significant. 4The positive expression rates of Bcl-2 and p53 proteins in 46 cases of lymphoma were not significantly different between the age group 鈮，

本文編號：1516931