發(fā)布時(shí)間：2021-08-07 09:21

　　22周齡人胎肝是胎兒造血的主要場(chǎng)所,此階段是造血系統(tǒng)遷入和遷出的關(guān)鍵轉(zhuǎn)折點(diǎn)。這一獨(dú)特性使其極具研究?jī)r(jià)值�；趯�(duì)22周齡人胎肝cDNA進(jìn)行大規(guī)模測(cè)序的結(jié)果,采用生物信息學(xué)方法和實(shí)驗(yàn)手段（cDNA末端快速擴(kuò)增法等）獲得了13條具有重要功能線索的人源新基因的22條全長(zhǎng)cDNA序列。所有這些序列均率先在GenBank注冊(cè)。關(guān)于其中部分基因的主要研究結(jié)果如下: 人源Semaphorin 6C和Semaphorin 6D:克隆了Semaphorin基因家族的兩個(gè)新成員SEMA6C和SEMA6D,它們都屬于膜結(jié)合型的第六亞家族。這兩個(gè)基因分別由20個(gè)和19個(gè)外顯子組成,染色體定位分別為1q12-21.1和15q21.1。關(guān)于SEMA6C和SEMA6D,分別發(fā)現(xiàn)了3種和5種不同剪切形式,它們的表達(dá)與組織特異性及發(fā)育階段有關(guān)。Northern雜交結(jié)果顯示,SEMA6C主要在骨骼肌中表達(dá),SEMA6D則在腎、腦和胎盤(pán)中高表達(dá),在心肌和骨骼肌中也有一定量的表達(dá)。缺失體研究表明,其胞外結(jié)構(gòu)域（Sema結(jié)構(gòu)域和PSI結(jié)構(gòu)域）的完整性是Semaphorin蛋白進(jìn)行適當(dāng)?shù)姆g后修飾和亞細(xì)胞定位所必須的。SEM... 

【文章來(lái)源】：中國(guó)人民解放軍軍事科學(xué)院北京市

【文章頁(yè)數(shù)】：120 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
中文摘要
英文摘要
Chapter 1 Identification, Characterization and Functional Study of the Two Novel Human Members of Semaphorin Gene Family
    1. Introduction
    2. Materials and Methods
    3. Results
        3.1 Identification and Cloning of Human Semaphorin SEMA6C and SEMA6D
        3.2 The Genomic Structures and Chromosomal Localization of the Genes for Human SEMA6C and SEMA6D
        3.3 Distribution of SEMA6C and SEMA6D mRNAs
        3.4 Alternative Splicing of SEMA6C and SEMA6D
        3.5 Characterization of SEMA6C and SEMA6D
        3.6 Growth Cone Collapse Activity of SEMA6C and SEMA6D
    4. Discussion
    References
Chapter 2: Characterization and Expression of Three Novel Differentiation-related Genes Belong to the Human NDRG Gene Family
    1. Introduction
    2. Materials and Methods
    3. Results
        3.1 Identification and cloning of human genes NDRG2, NDRG3 and NDRG4
        3.2 Sequence analysis of human NDRG2, NDRG3 and NDRG4 proteins
        3.3 Chromosomal localization of the genes for human NDRG2, NDRG3 and NDRG4
        3.4 Transcription profile of NDRG2, NDRG3 and NDRG4
        3.5 Subcellular localization of NDRG2, NDRG3 and NDRG4 proteins
    4. Discussion
    References
Chapter 3: Characterization and Tissue Expression of a Novel Human Gene npdc1
    1. Introduction
    2. Materials and Methods
    3. Results and Discussion
        3.1 Identification and cloning of hnpdcl
        3.2 Sequence analysis of hNPDCl
        3.3 Chromosomal localization of the gene for hnpdcl
        3.4 Transcription profile of the hnpdcl gene in different human tissues
    References
Chapter 4: Cloning, Chromosomal Assignment and Tissue Expression of a Novel Human Protein Tyrosine Phosphatase Gene
    1. Introduction
    2. Materials and Methods
    3. Results and Discussion
        3.1 Identification and cloning of human PTP-TD14 gene
        3.2 Sequence analysis of human PTP-TD14
        3.3 Transcription profile of the human PTP-TD14 gene
        3.4 Chromosomal localization of the genes for human PTP-TD14
    References
Chapter 5: Cloning and Chromosomal Assignment of Six Novel Human Genes with Important Biological Functions
    1. Introduction
    2. Materials and Methods
    3. Results and Discussion
        3.1 Human BKLF gene
        3.2 Human c-Jun binding protein (JBP) gene
        3.3 Human hematopoietic zinc finger protein (Hzf) gene
        3.4 Human liver membrane-bound protein (LMP) gene
        3.5 Human liver nuclear protein LNP gene
        3.6 Human clathrin coat assembly protein (AP47) gene
    References
綜述 Semaphorin及其受體的研究進(jìn)展
附錄一 英文縮寫(xiě)名
附錄二 22 full-length cDNAs deposited in GenBank
附錄三 博士后期間申請(qǐng)(負(fù)責(zé))的基金項(xiàng)目和專利項(xiàng)目
附錄四 博士后期間完成的論文
致謝



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