哺乳動(dòng)物雷帕霉素靶蛋白（mammalian target of rapamycin, mTOR）是一種屬于PI3K家族高度保守的絲氨酸/蘇氨酸蛋白激酶,對(duì)細(xì)胞生長(zhǎng)和增殖起關(guān)鍵調(diào)節(jié)作用。它可接收生長(zhǎng)因子、營(yíng)養(yǎng)、能量等多種信號(hào),通過PI3K/Akt/mTOR途徑、氨基酸、能量和環(huán)境壓力信號(hào)來發(fā)揮作用。mTOR與不同蛋白結(jié)合形成兩個(gè)復(fù)合物：哺乳動(dòng)物雷帕霉素靶蛋白復(fù)合物1（mammalian Target of Rapamycin Complex1, mTORC1）和哺乳動(dòng)物雷帕霉素靶蛋白復(fù)合物2（mammalian Target of Rapamycin Complex2, mTORC2）.目前對(duì)mTORC1研究較多,在哺乳動(dòng)物中,mTORC1由Raptor、mTOR、mLST8、Deptor和PRAS40組成,主要與細(xì)胞生長(zhǎng)、自噬、凋亡蛋白質(zhì)合成以及代謝等相關(guān)。而對(duì)mTORC2研究較少,在哺乳動(dòng)物中,mTORC2由Rictor、mTOR、mLST8、PRR5和mSinl組成,其中Rictor是nTORC2特異性成分、支架蛋白以及發(fā)揮生物學(xué)作用必不可少的部分。已報(bào)道nTORC2主要功能為控制... 

【文章來源】：浙江大學(xué)浙江省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：84 頁

【學(xué)位級(jí)別】：碩士

【部分圖文】：

PP242對(duì)小鼠ES細(xì)胞體外定向心肌細(xì)胞分化的影響

浙江大學(xué)碩士學(xué)位論文 第一部分實(shí)驗(yàn)結(jié)果cytometry analysis in EBs on day 5+3. (D) Immunofluorescent analysis of a-Actinin in EBs treatedwith PP242 on d 5+3. DAPI staining indicates the location of cells. Similar data were obtained fromat least 3 independent experiments. Bar=25 jim. *P<0.05, **/^<0.01 uy control.

圖1.3 shRNA-/?/ctor和shRNA-Zftf/^tor對(duì)小鼠ES細(xì)胞體外定向心肌分化的影響。Fig.1.3 Knockdown of Rictor or Raptor effected cardiac differentiation of mouse ES cells invitro. (A) Percentage of beating EBs during differentiation from day 5+1 to day 5+3. The percentageof beating EBs was decreased by shRNA-Rictor, but it was accelerated by shRNA-i^i^p/o/*. (B) Flowcytometry analysis showed the percentage of a-Actinin-positive cells in the total population of EBson day 5+3. shRNA-/?/c7or significantly decreased the percentage of a-Actinin-positive cells, but

【參考文獻(xiàn)】：
期刊論文
[1]抑制PPAR-α表達(dá)對(duì)ET-1誘導(dǎo)的心肌肥大和PI3K/Akt/GSK3β-NFATc4通路的影響[J]. 李瑞芳,樂康,高潔,楊國(guó)慶,鮑穎霞,劉培慶.  中國(guó)病理生理雜志. 2009(12)



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