【摘要】： 目的研究內(nèi)嗎啡肽-1對正常骨髓基質(zhì)細(xì)胞表面細(xì)胞間黏附分子(intercellularadhesion molecule,ICAM-1)、血管細(xì)胞黏附分子(Vascular cell adhesion molecule,VCAM-1),和基質(zhì)細(xì)胞分泌的細(xì)胞因子IL-6、TNF-a的調(diào)控作用。 方法Ficoll密度梯度離心法分離骨髓基質(zhì)細(xì)胞,應(yīng)用流式細(xì)胞術(shù)檢測其表面標(biāo)志。流式細(xì)胞術(shù)檢測黏附分子ICAM-1和VCAM-1的表達(dá),酶聯(lián)免疫吸附法檢測細(xì)胞因子IL-6、TNF-a的濃度。 結(jié)果黏附分子的表達(dá):正常骨髓基質(zhì)細(xì)胞加入內(nèi)嗎啡肽-1培養(yǎng)24h后各實(shí)驗(yàn)組ICAM-1和VCAM-1表達(dá)與對照組比較有顯著性差異(P＜0.05),而在相同內(nèi)嗎啡肽-1濃度下,培養(yǎng)時(shí)間的的變化對于二者的表達(dá)無明顯影響;細(xì)胞因子的分泌水平:不管是量效關(guān)系還是時(shí)效關(guān)系,IL-6、TNF-a的濃度實(shí)驗(yàn)各組數(shù)據(jù)與對照組比較無顯著性差異(P＞0.05) 結(jié)論1內(nèi)嗎啡肽-1能調(diào)控正常骨髓基質(zhì)表面ICAM-1和VCAM-1的表達(dá)。 2內(nèi)嗎啡肽-1對于正常骨髓基質(zhì)細(xì)胞分泌的細(xì)胞因子IL-6、TNF-a無明顯促進(jìn)或抑制作用。
[Abstract]:Objective to investigate the effects of endomorphin 1 on intercellularadhesion molecule-1 (ICAM-1), (Vascular cell adhesion molecule-1 (VCAM-1) and the cytokine IL-6 / TNF-a secreted by stromal cells in normal bone marrow stromal cells. Methods Bone marrow stromal cells were isolated by Ficoll density gradient centrifugation and their surface markers were detected by flow cytometry. The expression of ICAM-1 and VCAM-1 was detected by flow cytometry and the concentration of cytokine IL-6 and TNF-a was detected by enzyme-linked immunosorbent assay (Elisa). Results the expression of adhesion molecules: the expression of ICAM-1 and VCAM-1 in normal bone marrow stromal cells cultured with endomorphin 1 for 24 hours was significantly different from that in control group (P < 0. 05), but at the same concentration of endomorphin 1, the expression of ICAM-1 and VCAM-1 in each experimental group was significantly higher than that in control group (P < 0. 05). The change of culture time had no significant effect on the expression of both. Levels of cytokines secretion: no significant difference was found in the concentration of IL-6 and TNF-a between the dose-response and time-dependent groups (P > 0.05). Conclusion 1 morphine peptide 1 can regulate the normal bone marrow base. The expression of ICAM-1 and VCAM-1 on the cytoplasm. 2 morphine peptide 1 had no obvious effect on the cytokine IL-6 and TNF-a secreted by normal bone marrow stromal cells.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R329

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