本文選題：創(chuàng)傷愈合 + 血管新生模式��； 參考：《蘇州大學(xué)》2009年碩士論文

【摘要】： 毛細(xì)血管是唯一進(jìn)行代謝物質(zhì)交換的場(chǎng)所,為機(jī)體各器官提供營(yíng)養(yǎng)物質(zhì),氧氣及置換代謝產(chǎn)物CO_2等,并傳送出體外。當(dāng)局部組織發(fā)生創(chuàng)傷后,毛細(xì)血管的新生既是組織自我修復(fù)完整性的一部分,也是組織進(jìn)一步修復(fù)的基礎(chǔ)條件。新毛細(xì)血管的形成對(duì)供養(yǎng)新近形成的組織是必需的。血管新生可以縮短氧從毛細(xì)血管向細(xì)胞彌散的距離,增加細(xì)胞氧的供應(yīng),從而改善細(xì)胞在缺氧條件下的功能,并進(jìn)而改善整個(gè)創(chuàng)傷組織的供氧情況。出芽和套疊是創(chuàng)傷愈合過(guò)程中的血管新生的主要方式,雖然創(chuàng)傷愈合中血管新生的研究已取得了諸多進(jìn)展,對(duì)套疊和出芽血管新生的具體過(guò)程、基本機(jī)制有了較多的了解,但對(duì)創(chuàng)傷愈合中血管新生詳細(xì)的機(jī)制仍然未被闡明。為了探索這些問(wèn)題的答案,本研究試圖通過(guò)提出毛細(xì)血管網(wǎng)絡(luò)構(gòu)建的一系列假設(shè),并依據(jù)內(nèi)皮細(xì)胞因子向周?chē)M織擴(kuò)散的模型,對(duì)內(nèi)皮細(xì)胞對(duì)于不同濃度的內(nèi)皮生長(zhǎng)因子的反應(yīng)進(jìn)行研究,以期探討血管新生的模式。在本文中主要通過(guò)以下幾個(gè)方面的工作開(kāi)展本課題的研究。 一、對(duì)創(chuàng)傷愈合中的血管新生研究的背景及研究對(duì)象相關(guān)的認(rèn)知現(xiàn)狀與基礎(chǔ)知識(shí)的梳理。由于血管新生伴隨著創(chuàng)傷愈合的整個(gè)過(guò)程,對(duì)創(chuàng)傷愈合的基本過(guò)程進(jìn)行了介紹。并詳細(xì)說(shuō)明了關(guān)于血管新生與創(chuàng)傷愈合的關(guān)系的研究。同時(shí)對(duì)近年來(lái)創(chuàng)傷愈合中的血管新生數(shù)學(xué)模型的研究進(jìn)行了總結(jié),提出這些模型存在的問(wèn)題,提出本論文研究的目的和意義。 二、分析與探討出芽新生和套迭新生的基本過(guò)程以及各自的調(diào)控機(jī)制,在出芽新生和套迭新生研究的基礎(chǔ)上,關(guān)注創(chuàng)傷愈合過(guò)程中的血管新生的機(jī)制和模式。 三、通過(guò)實(shí)驗(yàn)觀察毛細(xì)血管在創(chuàng)傷愈合中不同階段的生長(zhǎng)形態(tài)。在成年SD大鼠皮膚做深至全層的切割傷,在創(chuàng)傷后不同時(shí)相點(diǎn)取材,通過(guò)透射電鏡觀察毛細(xì)血管和內(nèi)皮細(xì)胞的形態(tài),來(lái)推測(cè)血管的新生模式。通過(guò)實(shí)驗(yàn)發(fā)現(xiàn)在創(chuàng)傷修復(fù)的過(guò)程中,創(chuàng)傷早期急性缺氧條件下毛細(xì)血管主要以出芽新生。在缺氧程度減緩的情況下,VEGF濃度下降,套迭新生出現(xiàn)。 四、基于創(chuàng)傷部位或缺氧源部位的細(xì)胞大量表達(dá)VEGF;血管內(nèi)皮細(xì)胞受體Flk-1和Flt-1增加;VEGF與受體特異性結(jié)合,促進(jìn)內(nèi)皮細(xì)胞的分裂增殖;VEGF濃度不同,內(nèi)皮細(xì)胞反應(yīng)不同等事實(shí),提出毛細(xì)血管網(wǎng)絡(luò)建構(gòu)的一些假設(shè),建立內(nèi)皮生長(zhǎng)因子向周?chē)M織擴(kuò)散的模型,并對(duì)影響內(nèi)皮生長(zhǎng)因子濃度的因素進(jìn)行分析和討論,根據(jù)內(nèi)皮細(xì)胞對(duì)不同濃度的內(nèi)皮生長(zhǎng)因子的應(yīng)答,構(gòu)建毛細(xì)血管的新生及其網(wǎng)絡(luò)形成模式。 通過(guò)這些研究,我們對(duì)于傷口愈合中毛細(xì)血管生長(zhǎng)的模式與內(nèi)皮細(xì)胞對(duì)不同濃度的內(nèi)皮生長(zhǎng)因子的反應(yīng)之間的關(guān)系提出了新的看法與模型,為獲得判斷傷口愈合中的血管新生的方式的依據(jù)問(wèn)題進(jìn)行了探索。
[Abstract]:Capillaries are the only places where metabolites are exchanged, which provide nutrients, oxygen and replacement metabolites COSP 2 for various organs of the body, and transport them out of the body. When local tissue is traumatized, capillary neovascularization is not only a part of tissue self-repair integrity, but also a basic condition for further tissue repair. The formation of new capillaries is necessary to support newly formed tissue. Angiogenesis can shorten the distance of oxygen diffusion from capillaries to cells, increase the supply of oxygen to cells, thus improve the function of cells under hypoxia, and then improve the oxygen supply of the whole wound tissue. Budding and intussusception are the main ways of angiogenesis in wound healing. Although many advances have been made in the study of angiogenesis in wound healing, the basic mechanism of angiogenesis in the process of intussusception and budding has been well understood. However, the detailed mechanism of angiogenesis in wound healing remains unexplained. In order to explore the answers to these questions, this study attempts to propose a series of assumptions for the construction of capillary networks, and based on the model of endothelial cytokine diffusion to surrounding tissues. The response of endothelial cells to different concentrations of endothelial growth factor (et) was studied in order to explore the pattern of angiogenesis. In this paper, mainly through the following aspects of work to carry out the study of this subject. First, the background of angiogenesis research in wound healing and the related cognitive status and basic knowledge. The basic process of wound healing is introduced because angiogenesis is accompanied by the whole process of wound healing. The relationship between angiogenesis and wound healing is described in detail. At the same time, the research of angiogenesis mathematical models in wound healing in recent years is summarized, the problems existing in these models are put forward, and the purpose and significance of this paper are put forward. Secondly, the basic process and regulation mechanism of budding and overlaying are analyzed and discussed. Based on the research of budding and overlaying, the mechanism and mode of angiogenesis in wound healing are paid more attention to. Third, the growth of capillaries in different stages of wound healing was observed experimentally. In adult SD rats, the skin was cut deep to the whole layer, and the morphology of capillary and endothelial cells was observed by transmission electron microscope (TEM) to predict the pattern of angiogenesis. It was found that in the process of wound repair, the capillary mainly sprout in the early stage of trauma under the condition of acute hypoxia. Under the condition of decreasing hypoxia, the concentration of VEGF decreased and the overlap appeared. (4) VEGF was expressed in large quantities in the cells of wound site or anoxic source, the vascular endothelial cell receptors Flk-1 and Flt-1 increased the specific binding of VEGF to the receptor, and promoted the proliferation of endothelial cells. The concentration of VEGF was different and the response of endothelial cells was different. Some hypotheses of capillary network construction were put forward, and a model of endothelial growth factor diffusion to surrounding tissues was established, and the factors affecting the concentration of endothelial growth factor were analyzed and discussed. According to the response of endothelial cells to different concentrations of endothelial growth factor, the capillary neovascularization and its network formation model were constructed. Through these studies, we have proposed a new view and model of the relationship between the capillary growth pattern in wound healing and the response of endothelial cells to different concentrations of endothelial growth factor. In order to obtain the basis for judging the way of angiogenesis in wound healing.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R641;R363

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 胡綺;多孔絲素膜材料中血管新生模式機(jī)制的探索[D];蘇州大學(xué);2012年

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本文編號(hào)：2019467