本文選題：銅綠假單胞菌 + Opr�。� 參考：《生物技術(shù)通報(bào)》2015年07期

【摘要】：銅綠假單胞菌外膜蛋白Opr I具有較強(qiáng)的免疫原性,在疫苗上具有開(kāi)發(fā)前景。利用分子方法獲得Opr I蛋白的表達(dá)菌株。Western blotting驗(yàn)證表明抗體能與Opr I蛋白特異性結(jié)合。SDS-PAGE電泳切膠純化獲得Opr I蛋白。將Opr I蛋白免疫小鼠并攻毒銅綠假單胞菌,發(fā)現(xiàn)Opr I蛋白激活的特異性免疫對(duì)小鼠銅綠假單胞菌感染的保護(hù)率達(dá)到57.14%,與對(duì)照組相比較達(dá)到顯著性。采用正交試驗(yàn)設(shè)計(jì),獲得Opr I菌株最佳誘導(dǎo)表達(dá)條件為:加IPTG菌夜OD600值0.8,加IPTG終濃度0.3 mmol/L,誘導(dǎo)時(shí)間8 h,誘導(dǎo)溫度28℃;菌株最佳培養(yǎng)條件為轉(zhuǎn)速230 r/min,葡萄糖濃度0%,裝液量50 m L。
[Abstract]:Pseudomonas aeruginosa outer membrane protein (Opr I) has strong immunogenicity and is promising in vaccine development. The expression strain of Opr I protein was obtained by molecular method. Western blotting analysis showed that the antibody could bind to Opr I protein specifically. SDS-PAGE gel electrophoresis was used to purify Opr I protein. Opr I protein was immunized against Pseudomonas aeruginosa in mice. It was found that the protective rate of specific immunity activated by Opr I protein against Pseudomonas aeruginosa infection was 57.14%, which was significantly higher than that in control group. By orthogonal design, the optimal expression conditions of Opr I were obtained as follows: the OD600 value of IPTG strain was 0.8, the final concentration of IPTG was 0.3 mmol / L, the induction time was 8 h, and the induction temperature was 28 鈩，

本文編號(hào)：1882475