煙酰胺腺嘌呤二核苷酸對實(shí)驗性自身免疫性腦脊髓炎小鼠SIRT3/AMPK/mTOR通路的影響
發(fā)布時間：2022-02-10 04:37
目的:研究煙酰胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleot-ide,NAD+）對實(shí)驗性自身免疫性腦脊髓炎（experimental autoimmune encephalomyelitis,EAE）小鼠腦組織中沉默信息調(diào)節(jié)因子3（Silent information regulator of transcription 3,SIRT3）表達(dá)的影響,探究NAD+對EAE小鼠發(fā)揮治療作用可能的通路及機(jī)制,為臨床多發(fā)性硬化的治療尋找新的思路。方法:1.選用C57BL/6雌性小鼠（周齡8-10周、體重18-20g）,以MOG35-55作為免疫抗原,輔以完全弗氏佐劑、結(jié)核菌素充分乳化,使結(jié)核桿菌H37Ra的終濃度為4mg/ml,于小鼠背部脊柱兩旁皮下注射,并分別于免疫的當(dāng)天（免疫當(dāng)天為第0天）及第2天,分兩次腹腔注射0.5ml百日咳毒素（PTX）,構(gòu)建EAE小鼠模型。2.將實(shí)驗小鼠隨機(jī)分為正常對照組（Control組）、EAE模型組、NAD+治療組,每組8只。免疫后的第1天起...
【文章來源】：河北醫(yī)科大學(xué)河北省
【文章頁數(shù)】：49 頁
【學(xué)位級別】：碩士
【部分圖文】：
NAD+降低EAE小鼠的Weaver’s評分Fig.1NAD+reducedWeaver’sscoresofmodel(EAE)mice.
圖 2 NAD+減輕 EAE 小鼠中樞神經(jīng)系統(tǒng)炎性細(xì)胞浸潤及脫髓鞘程度Fig.2 NAD+mitigates inflammation and demyelination in EAE mice.HE analysis of the spinal cord shows the infiltration of inflammatory ce white matter (a 50×, b 200×). The arrows indicate the inflammat. (B) LFB analysis of the spinal cord shows the areas of intact mye) and demyelination (white) (a 50×, b 200×). The arrows indicate yelination in the spinal cord. (C) HE analysis of the brain shows tration of inflammatory cells in the perivascular (200×). The arroate the inflammatory cells. (D) LFB analysis of the brain shows the artact myelin (blue) and demyelination (white) (50×). The arrows indicdemyelination in the spinal cord. (E) The mean pathological scores mmation and demyelination in Control、PBS-treated EAE mice +-treated mice were measured by semi-quantitative analysis. * P <0
行石蠟包埋切片后，進(jìn)行 SIRT3 免疫組織化學(xué)染色。結(jié)果如圖 3A 所E 組與 Control 組相比 SIRT3 陽性細(xì)胞表達(dá)數(shù)目明顯減少，而 NAT3 陽性細(xì)胞表達(dá)數(shù)較 EAE 組顯著增加。統(tǒng)計分析可得（圖 3B）：與 Control 組陽性細(xì)胞數(shù)分別為 2.83±1.169、16.17±4.708，差異具學(xué)意義（P<0.01）。NAD+組陽性細(xì)胞數(shù)為 23.83±4.997，與 EAE 組相異具有統(tǒng)計學(xué)意義（P<0.01）。AControl EAE NAD+
本文編號：3618263
【文章來源】：河北醫(yī)科大學(xué)河北省
【文章頁數(shù)】：49 頁
【學(xué)位級別】：碩士
【部分圖文】：
NAD+降低EAE小鼠的Weaver’s評分Fig.1NAD+reducedWeaver’sscoresofmodel(EAE)mice.
圖 2 NAD+減輕 EAE 小鼠中樞神經(jīng)系統(tǒng)炎性細(xì)胞浸潤及脫髓鞘程度Fig.2 NAD+mitigates inflammation and demyelination in EAE mice.HE analysis of the spinal cord shows the infiltration of inflammatory ce white matter (a 50×, b 200×). The arrows indicate the inflammat. (B) LFB analysis of the spinal cord shows the areas of intact mye) and demyelination (white) (a 50×, b 200×). The arrows indicate yelination in the spinal cord. (C) HE analysis of the brain shows tration of inflammatory cells in the perivascular (200×). The arroate the inflammatory cells. (D) LFB analysis of the brain shows the artact myelin (blue) and demyelination (white) (50×). The arrows indicdemyelination in the spinal cord. (E) The mean pathological scores mmation and demyelination in Control、PBS-treated EAE mice +-treated mice were measured by semi-quantitative analysis. * P <0
行石蠟包埋切片后，進(jìn)行 SIRT3 免疫組織化學(xué)染色。結(jié)果如圖 3A 所E 組與 Control 組相比 SIRT3 陽性細(xì)胞表達(dá)數(shù)目明顯減少，而 NAT3 陽性細(xì)胞表達(dá)數(shù)較 EAE 組顯著增加。統(tǒng)計分析可得（圖 3B）：與 Control 組陽性細(xì)胞數(shù)分別為 2.83±1.169、16.17±4.708，差異具學(xué)意義（P<0.01）。NAD+組陽性細(xì)胞數(shù)為 23.83±4.997，與 EAE 組相異具有統(tǒng)計學(xué)意義（P<0.01）。AControl EAE NAD+
本文編號：3618263
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