目的:探討苦參素（Oxymatrine,OMT）是否可促進(jìn)小鼠腦梗死后神經(jīng)功能恢復(fù)和軸突再生并探討其潛在機(jī)制。方法:將健康雄性C57BL/6小鼠隨機(jī)分為假手術(shù)組（Sham）、溶劑對照組（Vehicle）和OMT組,采用電凝法制備小鼠局灶性大腦中動脈皮層梗死模型。術(shù)后24 h腹腔注射不同劑量OMT溶液或等體積蒸餾水,1次/d,連續(xù)14天。術(shù)后3d、7d、14d、28d進(jìn)行Rota rod實(shí)驗(yàn)（Rota-rod test）和網(wǎng)格行走實(shí)驗(yàn)（Grid walking test）,檢驗(yàn)OMT促進(jìn)神經(jīng)功能恢復(fù)的效果并確定最佳劑量。術(shù)后14d給各組小鼠健側(cè)相對應(yīng)皮層注射生物素葡聚糖胺（biotinylated dextran amines,BDA）。術(shù)后28d取腦染色,觀察各組雙側(cè)皮層內(nèi)被BDA標(biāo)記神經(jīng)元胞體和突起的變化情況。術(shù)后3d、7d、14d、28d分別取腦組織,采用免疫熒光技術(shù)檢測各組小鼠梗死灶周圍皮層內(nèi)Gap-43的表達(dá)變化,采用免疫蛋白印跡法檢測各組小鼠梗死側(cè)腦組織內(nèi)Gap-43、BDNF及GDNF的表達(dá)變化。結(jié)果:1.行為學(xué)實(shí)驗(yàn)結(jié)果提示OMT可顯著提高腦梗死小鼠的運(yùn)動功能,降低其行走失... 

【文章來源】：河北醫(yī)科大學(xué)河北省

【文章頁數(shù)】：53 頁

【學(xué)位級別】：碩士

【部分圖文】：

苦參素結(jié)構(gòu)式

行為學(xué)測試結(jié)果

Gap-43 免疫熒光染色顯示，與 sham 組相比，vehicle 組和 OMT 組術(shù)后 Gap-43 陽性細(xì)胞表達(dá)均增多（P < 0.05），提示有軸突再生；與 vehicle組相比，OMT 組 Gap-43 陽性細(xì)胞在術(shù)后 7 天（P < 0.05）、14 天（P < 0.01）和 28 天（P < 0.05）表達(dá)增多，差異有統(tǒng)計(jì)學(xué)意義，提示 OMT 可持續(xù)促進(jìn)梗死灶周圍皮質(zhì)內(nèi)軸突再生。

【參考文獻(xiàn)】：
期刊論文
[1]Cerebral ischemia and neuroregeneration[J]. Reggie H.C.Lee,Michelle H.H.Lee,Celeste Y.C.Wu,Alexandre Couto e Silva,Harlee E.Possoit,Tsung-Han Hsieh,Alireza Minagar,Hung Wen Lin.  Neural Regeneration Research. 2018(03)
[2]Anti-inflammatory mechanism of oxymatrine in dextran sulfate sodium-induced colitis of rats[J]. Ping Zheng, Feng-Li Niu, Wen-Zhong Liu, Yao Shi, Lun-Gen Lu, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200127, China.  World Journal of Gastroenterology. 2005(31)
[3]Oxymatrine therapy for chronic hepatitis B:A randomized doubleblind and placebo-controlled multi-center trial[J]. Lun-Gen Lu Min-De Zeng Yi-Min Mao Ji-Qiang Li, Shanghai Institute of Digestive Disease, Renji Hospital,Shanghai Second Medical University, Shanghai 200001, China Mo-Bin Wan Cheng-Zhong Li, Changhai Hospital, Second Military Medical University, Shanghai 200433, China Cheng-Wei Chen Qing-Chun Fu, Liver Disease Research Center, Nanjing Military Command, Shanghai 200233, China Ji-Yao Wang Wei-Min She, Zhongshan Hospital,Fudan University, Shanghai 200032, China Xiong Cai, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China Jun Ye, Shanghai Putuo District Central Hospital, Shanghai 200062, China Xia-Qiu Zhou Hui Wang, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China Shan-Ming Wu Mei-Fang Tang, Shanghai General Hospital of Infectious Diseases, Shanghai 200083, China Jin-Shui Zhu Wei-Xiong Chen, Shanghai No.6 People’s Hospital, Shanghai 200230, China Hui-Quan Zhang, Shanghai Shibei Hospital, Shanghai 200073, China.  World Journal of Gastroenterology. 2003(11)



本文編號：3402988