【摘要】：目的觀察β-石竹烯在局灶性腦缺血再灌注損傷模型中對大鼠血腦屏障的影響。方法采用中動脈栓塞法建立大鼠局灶性腦缺血再灌注損傷模型,分別設假手術組、模型組、β-石竹烯組(34、102、306 mg·kg~(-1))和尼莫地平(4 mg·kg~(-1))組。2,3,7-三苯基氯化四氮唑(TTC)染色法測定大鼠的腦梗死體積,伊文思藍法觀察大鼠血腦屏障通透性的改變。免疫組化法觀察缺血核心區(qū)皮質(zhì)水通道蛋白-4(AQP-4)的陽性表達。Western blot法檢測缺血核心區(qū)claudin-5水平變化和occluding的表達。結果再灌24 h后,β-石竹烯組較模型組可不同程度地改善神經(jīng)缺損癥狀,降低神經(jīng)行為學評分,減小腦梗死體積,減少缺血側EB含量,減少AQP-4的表達,增加claudin-5和occluding的表達(P0.05,P0.01)。結論β-石竹烯可通過減少AQP-4的表達,上調(diào)claudin-5和occluding的表達來減輕腦缺血再灌注所導致的腦水腫,降低血腦屏障損傷。
[Abstract]:Objective to observe the effect of 尾-caryophyllene on blood-brain barrier in rats with focal cerebral ischemia-reperfusion injury. Methods the rat model of focal cerebral ischemia-reperfusion injury was established by middle artery embolic method. The rats were divided into three groups: sham operation group, model group, 尾-caryophyllene group (34102306 mg kg~ (- 1) and nimodipine (4 mg kg~ (- 1) group. 2, 3, 7-triphenyltetrazolium chloride (TTC) staining was used to determine the cerebral infarction volume of rats, and Evans blue method was used to observe the changes of blood-brain barrier permeability. The positive expression of aquaporin-4 (AQP-4) in the cortex of ischemic core was observed by immunohistochemistry, and the level of claudin-5 and the expression of occluding in ischemic core were detected by Western blot. Results compared with the model group, 尾-caryophyllene group could improve the symptoms of nerve defect, decrease the neurobehavioral score, reduce the volume of cerebral infarction, decrease the content of EB on ischemic side, decrease the expression of AQP-4 and increase the expression of claudin-5 and occluding in 尾-caryophyllene group 24 h after reperfusion (P0.05, P0.01). Conclusion 尾-caryophyllene can reduce brain edema and blood-brain barrier injury by reducing the expression of AQP-4 and up-regulating the expression of claudin-5 and occluding.
【作者單位】： 重慶醫(yī)科大學藥學院生化與分子藥理研究室;
【基金】：國家自然科學基金面上項目(81173066) 國家科技重大專項重大新藥創(chuàng)制(2010ZX09401-306-1-1) 重慶市自然科學基金重點項目(CSTC2009BA5086)
【分類號】：R743

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