【摘要】：目的觀察米諾環(huán)素對大鼠腦缺血再灌注(ischemia/reperfusion,I/R)損傷后小膠質(zhì)細(xì)胞的激活及神經(jīng)炎癥的影響,探討腺苷A_(2A)受體(adenosine A_(2A)receptor,A_(2A)R)在米諾環(huán)素調(diào)節(jié)小膠質(zhì)細(xì)胞活化中的作用機制。方法采用線栓法阻塞大腦中動脈制作大鼠I/R損傷模型,將30只SD大鼠隨機分為假手術(shù)組、I/R模型組及米諾環(huán)素組〔I/R造模時,米諾環(huán)素按體質(zhì)量3mg/kg(質(zhì)量濃度為100g/1mL)尾靜脈注射,每日2次〕。再灌注24h后,處死大鼠,取大鼠缺血側(cè)腦組織,采用小膠質(zhì)細(xì)胞特異性標(biāo)記抗體CD11b/c染色、抗A_(2A)R抗體免疫熒光以及雙標(biāo)染色檢測缺血灶周圍活化的小膠質(zhì)細(xì)胞和A_(2A)R蛋白的表達(dá)。Western blot法檢測缺血腦組織內(nèi)白介素-1β、-6(IL-1β、IL-6)及A_(2A)R蛋白的表達(dá)。結(jié)果 I/R模型組CD11b陽性的小膠質(zhì)細(xì)胞數(shù)明顯多于假手術(shù)組;與I/R模型組相比,米諾環(huán)素組IL-1β、IL-6及A_(2A)R蛋白表達(dá)下降(P0.05),活化的小膠質(zhì)細(xì)胞數(shù)目明顯減少。結(jié)論米諾環(huán)素可抑制局灶性腦I/R損傷所致的小膠質(zhì)細(xì)胞激活及神經(jīng)炎癥反應(yīng),其機制可能與下調(diào)A_(2A)R蛋白的表達(dá)有關(guān)。
[Abstract]:Objective to observe the effect of minocycline on the activation of microglia and neuroinflammation after cerebral ischemia-reperfusion (ischemia/reperfusion,I/R) injury in rats, and to explore the effect of adenosine A _ (2A) receptor (adenosine A _ (2A) receptor,) on the activation of microglia and neuroinflammation in rats. The mechanism of A _ (2A) R) in the regulation of microglia activation by minocycline. Methods 30 SD rats were randomly divided into three groups: sham operation group, I R model group and Minocyclovir group. Minocycline was injected into tail vein by body mass 3mg/kg (100g/1mL) twice a day. After 24 hours of reperfusion, the rats were killed and the ischemic brain tissue was taken from the rats. The microglia specific labeled antibody CD11b/c was used to stain. The expression of activated microglia and A2A R protein around ischemic foci were detected by immunofluorescence and double labeling staining. Western blot was used to detect the expression of IL-1 尾, IL-1 尾, IL-6 in ischemic brain tissue. Expression of IL-6 and A2A R protein. Results the number of CD11b positive microglia in the model group was significantly higher than that in the sham operation group. Compared with the model group, the expression of IL-1 尾, IL-6 and A2A R protein in minocyclic group decreased (P0.05), and the number of activated microglia decreased significantly. Conclusion Minocycline can inhibit the activation of microglia and neuroinflammatory response induced by focal cerebral I R injury, which may be related to the down-regulation of A2A R protein expression.
【作者單位】： 西南醫(yī)科大學(xué)附屬醫(yī)院神經(jīng)內(nèi)科;
【基金】：四川省教育廳科研項目(No.15ZA0168) 瀘州市科技計劃項目(No.2014S4506) 西南醫(yī)科大學(xué)附屬醫(yī)院課題(No.2015-PT-010)資助
【分類號】：R743.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 陶濤;秦文熠;李作孝;李小剛;;米諾環(huán)素對局灶性腦缺血大鼠腦可塑性的影響[J];四川大學(xué)學(xué)報(醫(yī)學(xué)版);2015年04期

2 陶濤;秦新月;徐廣會;;養(yǎng)血清腦顆粒對缺血/再灌注大鼠腦炎性因子表達(dá)和軸突再生的影響[J];重慶醫(yī)科大學(xué)學(xué)報;2013年06期

3 范海玲;陳翔;;腺苷及A_(2A)腺苷受體與缺氧缺血性腦病[J];醫(yī)學(xué)研究雜志;2011年02期

4 蔡志友;晏勇;陳然;;美滿霉素抑制血管性認(rèn)知功能損傷大鼠海馬星型膠質(zhì)細(xì)胞激活和神經(jīng)炎癥(英文)[J];Neuroscience Bulletin;2010年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王莉智;和榮麗;楊桂姣;張楠;劉成芳;;自主跑輪運動對阿爾茨海默病模型小鼠學(xué)習(xí)記憶能力和海馬內(nèi)炎性細(xì)胞因子表達(dá)的影響[J];中國運動醫(yī)學(xué)雜志;2017年04期

2 陶濤;付潔;劉永剛;李作孝;李小剛;;米諾環(huán)素抑制腦缺血再灌注后小膠質(zhì)細(xì)胞激活的機制研究[J];四川大學(xué)學(xué)報(醫(yī)學(xué)版);2017年02期

3 宋玉成;郭秀珍;王愛芹;;左乙拉西坦對癲癇患者血清膠質(zhì)纖維酸性蛋白水平、腦電活動及生活質(zhì)量的影響[J];腦與神經(jīng)疾病雜志;2017年01期

4 孫縵利;鄧海峰;王晉;馬玲;林玲;;美滿霉素對阿爾茨海默病模型大鼠認(rèn)知功能的影響研究[J];現(xiàn)代預(yù)防醫(yī)學(xué);2016年20期

5 孫縵利;鄧海峰;李明華;劉國良;常全忠;;美滿霉素對阿爾茨海默病大鼠認(rèn)知損傷及海馬BDNF、Bcl-2和Bax表達(dá)的影響[J];天津醫(yī)藥;2016年09期

6 曹燕;張t盼，

本文編號：2464114