發(fā)布時(shí)間：2019-04-03 14:55

【摘要】：目的:探討B(tài)Q-123對(duì)蛛網(wǎng)膜下腔出血(SAH)的治療作用及其機(jī)制。方法:120只雄性SD大鼠,分為假手術(shù)(Sham)組、SAH組、雷帕霉素組、BQ-123干預(yù)組。二次注血法制作SAH大鼠模型;鏡下觀察海馬區(qū)組織形態(tài)結(jié)構(gòu)變化;免疫組化法和RT-PCR法檢測(cè)海馬區(qū)雷帕霉素靶蛋白(m TOR)、自噬相關(guān)基因Beclin-1和微管相關(guān)蛋白1輕鏈(LC3)-Ⅱ的表達(dá);TUNEL法檢測(cè)細(xì)胞凋亡情況。結(jié)果:SAH組海馬區(qū)可見(jiàn)神經(jīng)細(xì)胞變性水腫和死亡;血管內(nèi)皮細(xì)胞腫脹、凝集,管腔受壓、狹窄明顯,管壁迂曲不平、縫隙連接斷裂、基膜松散、模糊甚至分離。雷帕霉素組大部分細(xì)胞排列整齊、細(xì)胞結(jié)構(gòu)完整;血管內(nèi)皮細(xì)胞腫脹、凝集,管腔受壓、狹窄程度減輕,管壁迂曲不平;BQ-123干預(yù)組結(jié)構(gòu)完整神經(jīng)細(xì)胞顯著增多;血管內(nèi)皮細(xì)胞凝集已少見(jiàn),膜結(jié)構(gòu)完整、清晰,基膜厚薄均勻。與SAH組比較,雷帕霉素組和BQ-123干預(yù)組海馬區(qū)m TOR表達(dá)降低,Beclin-1和LC3-Ⅱ表達(dá)增加,凋亡神經(jīng)細(xì)胞數(shù)量減少(P0.05)。結(jié)論:BQ-123可抑制SAH大鼠神經(jīng)細(xì)胞凋亡,其機(jī)制與調(diào)控m TOR-自噬信號(hào)途徑有關(guān)。
[Abstract]:Objective: to investigate the therapeutic effect and mechanism of BQ-123 on subarachnoid hemorrhage (SAH) with (SAH). Methods: 120 male SD rats were divided into sham-operated (Sham) group, SAH group, rapamycin group and BQ-123 intervention group. The rat model of SAH was made by twice blood injection, and the morphological changes of hippocampus were observed under microscope. The expression of (m TOR), autophagy-associated gene Beclin-1 and microtubule-associated protein 1 light chain (LC3-鈪，

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