【摘要】：目的:研究運(yùn)動(dòng)訓(xùn)練對(duì)腦梗死急性期大鼠神經(jīng)功能恢復(fù)及梗死邊緣區(qū)皮質(zhì)神經(jīng)細(xì)胞自噬及凋亡的影響。方法:建立大腦中動(dòng)脈閉塞再灌注模型,并分成運(yùn)動(dòng)訓(xùn)練組、對(duì)照組和假手術(shù)組。運(yùn)動(dòng)訓(xùn)練組大鼠給予運(yùn)動(dòng)訓(xùn)練;對(duì)照組與假手術(shù)組則不予以任何針對(duì)性訓(xùn)練。造模術(shù)后第3、7、14d采用改良神經(jīng)損傷程度評(píng)分(mNSS)對(duì)各組大鼠進(jìn)行功能評(píng)估,同時(shí)以尼氏染色法測(cè)量腦梗死體積,免疫熒光方法觀察梗死邊緣區(qū)皮質(zhì)自噬標(biāo)志物L(fēng)C3-Ⅱ及凋亡標(biāo)志物TUNEL的表達(dá)情況。結(jié)果:造模術(shù)后14d運(yùn)動(dòng)訓(xùn)練組大鼠mNSS及腦梗死體積均優(yōu)于對(duì)照組,差別具有顯著性意義(P0.05)。運(yùn)動(dòng)訓(xùn)練組大鼠梗死邊緣區(qū)皮質(zhì)LC3-Ⅱ陽(yáng)性細(xì)胞數(shù)在造模后14d少于對(duì)照組(P0.05)。相關(guān)性分析結(jié)果顯示LC3-Ⅱ陽(yáng)性細(xì)胞數(shù)與mNSS(r=0.901,P0.001)及梗死體積(r=0.832,P0.001)成正相關(guān)。TUNEL陽(yáng)性細(xì)胞數(shù)在造模后7、14d均少于對(duì)照組(P0.001),且44.6%的LC3-Ⅱ與TUNEL存在共定位。結(jié)論:運(yùn)動(dòng)訓(xùn)練能夠促進(jìn)腦梗死急性期大鼠神經(jīng)功能恢復(fù),其機(jī)制可能與其減少梗死邊緣區(qū)神經(jīng)細(xì)胞自噬及凋亡有關(guān)。
[Abstract]:Aim: to study the effects of exercise training on the recovery of neural function and the autophagy and apoptosis of cortical neurons in the acute phase of cerebral infarction in rats. Methods: middle cerebral artery occlusion reperfusion model was established and divided into exercise training group, control group and sham operation group. Rats in the exercise training group were given exercise training, while the control group and the sham operation group were not given any specific training. The function of rats in each group was evaluated by modified nerve injury score (mNSS) on the 3rd day and 14th day after operation, and the infarct volume was measured by Nissl's staining method. The expression of autophagy marker LC3- 鈪，

本文編號(hào)：2407613