【摘要】：不同時長的電脈沖高頻刺激(high frequency stimulation,HFS)對于腦神經(jīng)系統(tǒng)具有不同的作用.其中,數(shù)秒時長的短促HFS可通過"點燃"效應(yīng)制作動物癲癇模型,也可以產(chǎn)生長時間保持的突觸可塑性變化,而數(shù)分鐘以上的長時HFS卻可以安全地用于臨床的深部腦刺激,治療多種腦疾病.因此推測,持續(xù)的HFS可以改變短促刺激產(chǎn)生的效應(yīng).為了驗證此推測,在大鼠海馬CA1區(qū)的輸入軸突纖維Schaffer側(cè)支上,分別施加5 s和2 min兩種時長的100 Hz HFS,并監(jiān)測刺激結(jié)束后下游神經(jīng)元群體對于單脈沖測試的響應(yīng)電位,即群峰電位(population spike,PS).結(jié)果顯示,5 s短HFS結(jié)束時會緊跟后放電癇樣活動,并且,從測試脈沖誘發(fā)的PS幅值和潛伏期可見,短HFS誘導的興奮性增強可以維持數(shù)十分鐘.反之,2 min的長HFS結(jié)束時緊隨之后的是數(shù)十秒無發(fā)放活動的靜息期,而且,PS在數(shù)分鐘內(nèi)即恢復到HFS前的基線水平.這些結(jié)果表明,長時HFS的后期刺激可以改變前期短促刺激對于下游神經(jīng)網(wǎng)絡(luò)的作用,即消除短刺激可能產(chǎn)生的長時程興奮效應(yīng).此發(fā)現(xiàn)對于深入了解高頻刺激的作用機制、促進深部腦刺激的臨床應(yīng)用具有重要意義.
[Abstract]:High frequency electrical pulse stimulation (high frequency stimulation,HFS) with different duration has different effects on the cerebral nervous system. Among them, several seconds of short HFS can be used to make animal model of epilepsy by "kindling" effect, and can also produce long-term synaptic plasticity changes, while long-term HFS for more than a few minutes can be safely used in clinical deep brain stimulation. Treat a variety of brain diseases. Therefore, it is speculated that sustained HFS can alter the effects of short-term stimuli. To verify this hypothesis, the lateral branches of the input axon fiber Schaffer in the CA1 region of the hippocampus of the rat were treated with 100 Hz HFS, for 5 s and 2 min, respectively, and the response potentials of the downstream neuronal population to monopulse test were monitored after the end of the stimulation. Group peak potential (population spike,PS). The results showed that the epileptiform activity was closely followed at the end of 5 s short HFS, and the enhancement of excitability induced by short HFS could be maintained for tens of minutes from the amplitude and latency of PS induced by the test pulse. On the other hand, the long HFS of 2 min was followed by a few seconds of inactive activity at the end of the HFS period, and the PS returned to its baseline level before HFS within a few minutes. These results indicate that the late stimulation of long term HFS can change the effect of short stimulus on the downstream neural network, that is, to eliminate the long term excitatory effect of short stimulus. The findings are of great significance in understanding the mechanism of high frequency stimulation and promoting the clinical application of deep brain stimulation.
【作者單位】： 浙江大學生物醫(yī)學工程與儀器科學學院生物醫(yī)學工程教育部重點實驗室;
【基金】：國家自然科學基金資助項目(30970753)~~
【分類號】：R741

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