【摘要】：目的探討瞬時受體電位通道1(TRPC1)沉默對腦缺血大鼠內源性神經(jīng)干細胞增殖、遷移和分化的影響。方法 SD大鼠分為假手術組、腦缺血組、TRPC1沉默組(腦缺血+TRPC1沉默)和陰性對照組,以腦室內注射siRNA沉默TRPC1,以線栓法制作大鼠大腦中動脈腦缺血(MCAO)模型,腦缺血模型制作成功后,腹腔內注射Brdu標記內源性神經(jīng)干細胞,分別于48 h、4 w后處死大鼠,行Brdu免疫組化染色、免疫熒光雙標染色(Brdu/GFAP、Brdu/Neun)觀察NSC的增殖、遷移和分化情況。結果腦缺血后48 h,腦缺血組和TRPC1沉默組SVZ區(qū)Brdu陽性表達均較假手術組明顯增多(P0.01),但TRPC1沉默組Brdu陽性細胞數(shù)較缺血組低(P0.01)。腦缺血4 w后,缺血組與假手術組相比,皮質區(qū)具有更多的Brdu陽性細胞(P0.01),同樣TRPC1沉默組Brdu陽性細胞數(shù)多于假手術組,但明顯低于腦缺血組(P0.01);免疫熒光雙標染色發(fā)現(xiàn),腦缺血各組Brdu/GFAP、Brdu/Neun雙陽性細胞的數(shù)量均比假手術組明顯增高,但TRPC沉默組雙陽性細胞顯著少于腦缺血組和陰性對照組(P0.01)。結論 TRPC1沉默顯著影響腦缺血大鼠SVZ區(qū)NSC的增殖、向腦缺血區(qū)遷移及向成熟細胞的分化。
[Abstract]:Objective to investigate the effects of transient receptor potential channel 1 (TRPC1) silencing on the proliferation, migration and differentiation of endogenous neural stem cells (NSCs) in rats with cerebral ischemia. Methods SD rats were divided into three groups: sham operation group, cerebral ischemia group, TRPC1 silencing group (cerebral ischemia TRPC1 silencing) and negative control group. The rat middle cerebral artery ischemic (MCAO) model was made by intraventricular injection of siRNA silencing TRPC1, with thread embolization. After the model of cerebral ischemia was established successfully, Brdu was injected intraperitoneally to label endogenous neural stem cells. The rats were killed at 48 h or 4 weeks later. The proliferation of NSC was observed by Brdu immunohistochemical staining and double immunofluorescence staining (Brdu/GFAP,Brdu/Neun). Migration and differentiation. Results at 48 hours after cerebral ischemia, the positive expression of Brdu in SVZ in cerebral ischemia group and TRPC1 silencing group was significantly higher than that in sham operation group (P0.01), but the number of Brdu positive cells in TRPC1 silencing group was lower than that in ischemic group (P0.01). After 4 weeks of cerebral ischemia, there were more Brdu positive cells in the cortex in the ischemic group than in the sham operation group (P0.01). The number of Brdu positive cells in the same TRPC1 silencing group was higher than that in the sham operation group, but significantly lower than that in the ischemic group (P0.01). Immunofluorescence double labeling staining showed that the number of Brdu/GFAP,Brdu/Neun double positive cells in cerebral ischemia group was significantly higher than that in sham operation group, but the number of double positive cells in TRPC silencing group was significantly lower than that in cerebral ischemia group and negative control group (P0.01). Conclusion TRPC1 silencing has a significant effect on the proliferation of NSC in the SVZ area, migration to the ischemic area and differentiation into mature cells of cerebral ischemia rats.
【作者單位】： 連云港市第一人民醫(yī)院神經(jīng)內科;重慶醫(yī)科大學附屬第一醫(yī)院神經(jīng)內科;
【基金】：中國博士后科學基金(No.2016M601759) 連云港市第一人民醫(yī)院博士科研啟動基金
【分類號】：R743.3

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