【摘要】：目的探索膠質(zhì)瘤細(xì)胞來源的管道(glioma clls derived vessels,GCDV)的形成機(jī)制。方法將膠質(zhì)瘤細(xì)胞株U87、U251、U373、SF295、T98G、SKMG-4和C6進(jìn)行體外三維培養(yǎng),觀察其管道形成能力。Western blot檢測(cè)各個(gè)膠質(zhì)瘤細(xì)胞株Notch1、Dll4蛋白的表達(dá)情況。結(jié)果三維培養(yǎng)C6細(xì)胞單個(gè)視野下(100×)的平均管道數(shù)(25.2±5.0)個(gè),U373為(36.4±3.20)個(gè),U87為(19.0±2.2)個(gè),T98G為(12.6±2.4)個(gè),SF295為(4.0±2.)個(gè),U251為(0.2±0.4)個(gè),SKMG-4為0。Notch1在U87、U251、T98G、SF295、SKMG-4、C6、U373表達(dá)相關(guān)密度分別為0.34、0.21、0.79、0.04、0.28、1.75、1.19,與管道形成能力顯著相關(guān)(r=0.778,P=0.019);Dll4表達(dá)相關(guān)密度與管道形成能力不相關(guān)(r=0.635,P=0.062)。結(jié)論 Notch1蛋白表達(dá)與細(xì)胞株管道形成能力密切相關(guān),而Dll4蛋白的表達(dá)與細(xì)胞株管道形成能力有待進(jìn)一步探索。
[Abstract]:Objective to explore the formation mechanism of (glioma clls derived vessels,GCDV in glioma cells. Methods Glioma cell line U87U251U373F295T98GNSKMG-4 and C6 were cultured in vitro, and the expression of Notch1,Dll4 protein in each glioma cell line was detected by. Western blot. Results the average number of tubes (25.2 鹵5.0), U373 (36.4 鹵3.20), U87 (19.0 鹵2.2), T98G (12.6 鹵2.4) and SF295 (4.0 鹵2. 2) in a single field of view were (25.2 鹵5.0), (36.4 鹵3.20), (19.0 鹵2.2), (12.6 鹵2.4) and (4.0 鹵2. 2) respectively. The correlation densities of U251, U251 and 0.Notch1 in U87U U251U T98GU SF295N SKMG-4, C6U U373 were 0.34, 0.21, 0.79, 0.04, 0.280.28 and 1.751.19, respectively, which were significantly correlated with the ability of pipeline formation (r = 0.778, P < 0.05), and the correlation densities of the expression of U251 and 0.Notch1 were 0.34, 0.21, 0.79, 0.04, 0.28 and 1.751.19, respectively. P0. 019); There was no correlation between the density of Dll4 expression and the ability of pipeline formation (r = 0.635P0. 062). Conclusion the expression of Notch1 protein is closely related to the ability of the cell line to form the conduit, while the expression of Dll4 protein and the ability of the cell line to form the conduit need to be further explored.
【作者單位】： 深圳市南山區(qū)人民醫(yī)院神經(jīng)外科;中山大學(xué)腫瘤防治中心神經(jīng)外科;中山大學(xué)附屬第八醫(yī)院深圳市福田區(qū)人民醫(yī)院神經(jīng)外科;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):81372685) 國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃)(編號(hào):2015CB755505) 廣東省自然科學(xué)基金(編號(hào):S2013040012894) 廣東省科技計(jì)劃項(xiàng)目(編號(hào):2013B021800067,2016A02013004) 深圳市科技創(chuàng)新委員會(huì)項(xiàng)目(編號(hào):JCYJ20140416094330210) 廣東省醫(yī)學(xué)科研基金(編號(hào):A2014247)
【分類號(hào)】：R739.41

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王建鵬;;Down-regulation of Notch-1 gene expression inhibits growth of TJ-905 glioblastoma[J];China Medical Abstracts(Surgery);2011年03期

2 程凱;;Notch:神經(jīng)分化與再生的關(guān)鍵基因[J];中國(guó)醫(yī)療前沿;2011年06期

3 袁梅;夏健;黃清;周琳;周芝文;后穎;楊期東;;轉(zhuǎn)染突變型Notch3基因?qū)�粒體融合蛋白-2表達(dá)及下游信號(hào)通路的影響[J];國(guó)際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2012年02期

4 余肖;張文迪;寧琴;羅小平;;MicroRNA-34a Inhibits Human Brain Glioma Cell Growth by Downregulation of Notch1[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2012年03期

5 藍(lán)佛琳;楊衛(wèi)忠;石松生;;Notch信號(hào)通路與腦膠質(zhì)瘤的研究進(jìn)展[J];福建醫(yī)藥雜志;2008年04期

6 呂文英;熊利澤;董海龍;張麗娜;張昊鵬;王世全;王叢叢;郭超;;Notch信號(hào)在肢體遠(yuǎn)程預(yù)處理誘導(dǎo)腦保護(hù)機(jī)制研究[J];中華神經(jīng)外科疾病研究雜志;2011年03期

7 郭娜;何金婷;胥桂華;王姣琦;李宗樹;莽靖;徐忠信;;Notch1受體在腦缺血損傷中表達(dá)變化及作用的體外研究[J];中風(fēng)與神經(jīng)疾病雜志;2013年04期

8 王力峰;陳麗;彭道珍;鐘茂林;;Notch信號(hào)通路在腦缺血損傷中的作用機(jī)制研究進(jìn)展[J];實(shí)用醫(yī)學(xué)雜志;2011年23期

9 鄭名哲;陳銜城;湯海亮;謝清;宮曄;;Notch信號(hào)通路基因在人腦動(dòng)靜脈畸形和周圍腦組織中的差異表達(dá)[J];中國(guó)臨床神經(jīng)科學(xué);2012年06期

10 易海波;石松生;楊衛(wèi)忠;;Notch信號(hào)通路與腦腫瘤[J];國(guó)際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2005年06期

相關(guān)會(huì)議論文 前4條

1 ;Regulation of Notch target gene Hesl expression by TGF-α in human glioma cell line U251[A];Proceedings of the 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience[C];2007年

2 何心;焦保華;梁朝輝;田偉;;shRNA靶向干擾Notch2對(duì)膠質(zhì)瘤生長(zhǎng)的體外和體內(nèi)研究[A];2011中華醫(yī)學(xué)會(huì)神經(jīng)外科學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2011年

3 鄭日亮;欒興華;呂鶴;劉e，

本文編號(hào)：2313000