【摘要】：目的觀察施萬樣細(xì)胞對(duì)坐骨神經(jīng)損傷(sciatic nerve injury,SNI)大鼠脊神經(jīng)節(jié)NGF和BDNF表達(dá)的影響,初步探討施萬樣細(xì)胞對(duì)脊神經(jīng)節(jié)的保護(hù)作用。方法先將脂肪源性干細(xì)胞(adipose-derived stem cells,ADSCs)誘導(dǎo)分化為施萬樣細(xì)胞并對(duì)后者進(jìn)行鑒定,后將二者分別植入脫細(xì)胞神經(jīng)移植物(ANA)中,構(gòu)建組織工程神經(jīng)。大鼠隨機(jī)分為正常對(duì)照組、ADSC組和施萬樣細(xì)胞組。后兩組建立SNI模型并用相應(yīng)的組織工程神經(jīng)橋接損傷的神經(jīng)。術(shù)后4周采用Western Blot和Real-time PCR檢測(cè)各組大鼠脊神經(jīng)節(jié)神經(jīng)生長因子(nerve growth factor,NGF)和腦源性神經(jīng)營養(yǎng)因子(brainderived neurotrophic factor,BDNF)蛋白和m RNA的表達(dá)。結(jié)果 ADSCs能夠誘導(dǎo)分化為施萬樣細(xì)胞并表達(dá)施萬細(xì)胞標(biāo)記物S100β和GFAP蛋白。施萬樣細(xì)胞組大鼠脊神經(jīng)節(jié)內(nèi)NGF和BDNF蛋白及m RNA表達(dá)量均高于ADSC組(P0.05)。結(jié)論施萬樣細(xì)胞可上調(diào)脊神經(jīng)節(jié)NGF和BDNF的表達(dá),對(duì)SNI所致的脊神經(jīng)節(jié)內(nèi)神經(jīng)元損傷有保護(hù)作用。
[Abstract]:Objective to observe the effects of Schwann like cells on the expression of NGF and BDNF in spinal ganglion of rats with sciatic nerve injury (sciatic nerve injury,SNI) and to explore the protective effect of Schwann like cells on spinal ganglion. Methods Adipose-derived stem cells (adipose-derived stem cells,ADSCs) were induced to differentiate into Schwan-like cells and identified. Then they were implanted into acellular nerve grafts (ANA) to construct tissue engineering nerves. Rats were randomly divided into normal control group (ADSC) and Schwann like cell group. The SNI model was established in the latter two groups and the injured nerve was bridged with the corresponding tissue engineering nerve. Western Blot and Real-time PCR were used to detect the expression of (nerve growth factor,NGF and (brainderived neurotrophic factor,BDNF protein and m RNA in the spinal ganglion of rats 4 weeks after operation. Results ADSCs could induce differentiation into Schwan-like cells and express S100 尾 and GFAP protein. The expression of NGF and BDNF protein and m RNA in spinal ganglion were higher in Schwann like cell group than in ADSC group (P0.05). Conclusion Schwann like cells can up-regulate the expression of NGF and BDNF in spinal ganglion and protect the neurons in spinal ganglion induced by SNI.
【作者單位】： 承德醫(yī)學(xué)院解剖教研室;承德市婦幼保健院;承德醫(yī)學(xué)院基礎(chǔ)所;
【基金】：河北省教育廳資助項(xiàng)目(QN2014138) 河北省重點(diǎn)發(fā)展學(xué)科“人體解剖與組織胚胎學(xué)”建設(shè)資助項(xiàng)目
【分類號(hào)】：R745

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