【摘要】：目的:研究濾泡輔助性T細(xì)胞(Tfh)在自身免疫性腦炎(AE)患者外周血中的百分比及臨床意義。方法:(1)采用自身免疫性腦炎相關(guān)抗體檢測(cè)陽性的患者腦脊液與大鼠腦組織冰凍切片共孵育并進(jìn)行免疫熒光染色,觀察結(jié)果;(2)收集血清和/或腦脊液(CSF)檢查自身免疫性腦炎相關(guān)抗體陽性的患者17例(其中,3例為抗LGI1抗體陽性患者,3例為抗GABAR抗體陽性患者,10例為抗NMDAR抗體陽性患者,1例為抗NMDAR抗體、抗GABAR抗體及抗AMPA2抗體合并陽性患者),收集外周血樣本共計(jì)21例次:未治療組6例次,治療組15例次(其中,治療后緩解組8例次,治療后未緩解組7例次),健康對(duì)照者(對(duì)照組)外周血15例。采用流式細(xì)胞術(shù)(FCM)檢測(cè)外周血單個(gè)核細(xì)胞(PBMC)中CD3+CD4+CXCR5+PD1+(即Tfh細(xì)胞)的比例,并分析Tfh細(xì)胞比例與患者疾病恢復(fù)程度的相關(guān)性。結(jié)果:(1)自身免疫性腦炎相關(guān)抗體檢測(cè)陽性患者腦脊液與大鼠腦組織冰凍切片共孵育免疫熒光染色結(jié)果呈現(xiàn)明顯陽性反應(yīng);(2)流式細(xì)胞術(shù)檢測(cè)結(jié)果顯示未治療組患者外周血Tfh比例明顯高于對(duì)照組(P0.001),且治療未緩解組患者Tfh比例高于治療緩解組(P0.05),而治療緩解組患者Tfh比例與對(duì)照組比較,差異無統(tǒng)計(jì)學(xué)意義(P=0.107)。結(jié)論:自身免疫性腦炎患者外周血Tfh比例升高可能與疾病的發(fā)生發(fā)展相關(guān)。
[Abstract]:Objective: to study the percentage and clinical significance of follicular helper T cell (Tfh) in peripheral blood of patients with autoimmune encephalitis (AE). Methods: (1) the cerebrospinal fluid (CSF) of patients with autoimmune encephalitis related antibody was incubated with frozen sections of rat brain tissue and immunofluorescence staining was performed. (2) 17 cases of autoimmune encephalitis related antibody positive by (CSF) in serum and / or cerebrospinal fluid were collected (3 cases were positive for anti LGI1 antibody, 3 cases were positive for anti GABAR antibody, 10 cases were positive for anti NMDAR antibody) One patient with anti NMDAR antibody, The peripheral blood samples were collected for 21 times: 6 cases in the untreated group, 15 times in the treatment group (including 8 cases in the post-treatment remission group). After treatment, there were 7 cases in the unrelieved group and 15 cases in the healthy control group (the control group). The proportion of CD3 CD4 CXCR5 PD1 (Tfh cells) in peripheral blood mononuclear cells (PBMC) was detected by flow cytometry (FCM), and the correlation between the proportion of Tfh cells and the degree of disease recovery was analyzed. Results: (1) the results of immunofluorescence staining in cerebrospinal fluid (CSF) incubated with frozen sections of rat brain tissue were obviously positive, and (2) flow cytometry showed that the patients in untreated group showed positive reaction. The proportion of Tfh in peripheral blood was significantly higher than that in control group (P0.001), and the proportion of Tfh in non-remission group was higher than that in remission group (P0.05), while the proportion of Tfh in treatment group was higher than that in control group. The difference was not statistically significant (P = 0.107). Conclusion: the increased proportion of Tfh in peripheral blood of patients with autoimmune encephalitis may be related to the occurrence and development of the disease.
【作者單位】： 第四軍醫(yī)大學(xué)基礎(chǔ)部免疫學(xué)教研室;第四軍醫(yī)大學(xué)西京醫(yī)院神經(jīng)內(nèi)科;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(No.81171977)資助
【分類號(hào)】：R742.9
，

本文編號(hào)：2217706