【摘要】：目的探討用于亨廷頓病(HD)治療提供的藥物篩選模型。方法 SD大鼠45只,分為正常對(duì)照組、毀損組、移植組三組,每組15只。正常組不予以處理,其余兩組均用奎寧酸(QA)腦內(nèi)注射,移植組在QA毀損模型建立后進(jìn)行人誘導(dǎo)多能干細(xì)胞(hiPSC)移植。hiPSC在大鼠腦內(nèi)存活了7 w,通過阿撲嗎啡誘導(dǎo)旋轉(zhuǎn)實(shí)驗(yàn)等行為學(xué)實(shí)驗(yàn),觀察各組大鼠運(yùn)動(dòng)功能情況;通過高十字迷宮、新環(huán)境抑制進(jìn)食實(shí)驗(yàn),觀察各組焦慮類似的行為變化。結(jié)果與QA組相對(duì)比,觀察到hiPSC移植組大鼠運(yùn)動(dòng)功能得到改善,大鼠焦慮類似的行為明顯減少。hiPSC移植組大鼠的活動(dòng)減少。結(jié)論hiPSC移植可以有效的改善HD大鼠的行為缺陷,hiPSC在治療神經(jīng)退行性疾病特別是HD,是一種理想的多潛能干細(xì)胞來源。
[Abstract]:Objective to investigate the drug screening model for (HD) treatment of Huntington's disease. Methods 45 SD rats were divided into normal control group, lesion group and transplantation group with 15 rats in each group. No treatment was given in the normal group, and the other two groups were injected with (QA). In the transplantation group, after the establishment of the model of QA damage, (hiPSC) transplantation of human induced pluripotent stem cells. HiPSC survived in the rat brain for 7 weeks. The motor function of each group was observed by behavioral experiments such as apomorphine induced rotation test, and the motor function was observed by high cross labyrinth. The new environment inhibited eating experiment and observed similar behavior changes of anxiety in each group. Results compared with the QA group, the motor function of the rats in the hiPSC transplantation group was improved, and the activity of the rats in the hiPSC transplantation group was significantly decreased. Conclusion hiPSC transplantation is an ideal source of multipotential stem cells for the treatment of neurodegenerative diseases, especially HD,.
【作者單位】： 武漢市第三醫(yī)院;武漢市武昌醫(yī)院;
【基金】：湖北省自然科學(xué)基金資助項(xiàng)目(面上基金)(No.2014CFB450) 武漢市衛(wèi)生計(jì)生委臨床醫(yī)學(xué)科研資助項(xiàng)目(No.wx15D21)
【分類號(hào)】：R-332;R742.2

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