【摘要】：目的：觀察豐富環(huán)境對HIBD新生大鼠海馬CA1區(qū)BDNF及細(xì)胞凋亡的影響,探討豐富環(huán)境治療HIBD的相關(guān)機(jī)制。方法：采用隨機(jī)數(shù)字表法將7日齡Sprague-Dawley清潔級新生大鼠分為假手術(shù)組、模型組及豐富環(huán)境組,各組又分為術(shù)后14d、28d兩個(gè)亞組(n=6)。參照Rice-Vannucci經(jīng)典方法改良建立新生大鼠HIBD動(dòng)物模型。模型組及假手術(shù)組置于標(biāo)準(zhǔn)環(huán)境中飼養(yǎng),豐富環(huán)境組予以豐富環(huán)境干預(yù)治療。術(shù)后14d、28d應(yīng)用Morris水迷宮試驗(yàn)檢測大鼠學(xué)習(xí)記憶能力,免疫組化法檢測大鼠海馬CA1區(qū)BDNF蛋白的陽性表達(dá)情況,TUNEL法檢測海馬CA1區(qū)細(xì)胞凋亡情況。結(jié)果：1.各組大鼠學(xué)習(xí)記憶能力：在術(shù)后14d、28d時(shí)間點(diǎn)上,與假手術(shù)組相比,模型組逃避潛伏期延長(P0.05),穿越平臺次數(shù)減少(P0.05),差異具有統(tǒng)計(jì)學(xué)意義；與模型組相比,豐富環(huán)境組逃避潛伏期縮短(P0.05),穿越平臺次數(shù)增多(P0.05),差異具有統(tǒng)計(jì)學(xué)意義。2.各組大鼠BDNF蛋白陽性表達(dá)變化：在術(shù)后14d、28d時(shí)間點(diǎn)上,與假手術(shù)組相比,模型組大鼠海馬CA1區(qū)可見BDNF蛋白陽性表達(dá)升高,差異具有統(tǒng)計(jì)學(xué)意義(P0.0 5)；與模型組相比,豐富環(huán)境組大鼠海馬CA 1區(qū)BDNF蛋白陽性表達(dá)更加明顯,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。3.各組大鼠凋亡細(xì)胞陽性表達(dá)變化：在術(shù)后14d、28 d時(shí)間點(diǎn)上,與假手術(shù)組相比,模型組凋亡細(xì)胞陽性表達(dá)升高,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；與模型組相比,豐富環(huán)境組凋亡細(xì)胞陽性表達(dá)顯著下降,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論：1.豐富環(huán)境可以改善HIBD大鼠學(xué)習(xí)記憶的能力。2.豐富環(huán)境可以上調(diào)HIBD大鼠海馬CA 1區(qū)BDNF蛋白表達(dá),下調(diào)HIBD大鼠海馬CA1區(qū)凋亡細(xì)胞陽性表達(dá)。
[Abstract]:Aim: to observe the effect of rich environment on BDNF and apoptosis in CA1 area of hippocampus of neonatal HIBD rats and to explore the mechanism of rich environment therapy on HIBD. Methods: the 7-day-old Sprague-Dawley clean newborn rats were randomly divided into three groups: sham operation group, model group and rich environment group. Each group was divided into two subgroups: 14 days after operation and 28 days after operation (nong6). The HIBD animal model of newborn rats was established by reference to the classical Rice-Vannucci method. Model group and sham operation group were fed in standard environment, rich environment group was given rich environment intervention therapy. Morris water maze test was used to detect the learning and memory ability of rats and the positive expression of BDNF protein in hippocampus CA1 area was detected by immunohistochemical method. Tunel method was used to detect apoptosis in CA1 area of hippocampus. The result is 1: 1. Learning and memory ability of rats in each group: at the time point of 14 days and 28 days after operation, compared with the sham operation group, the escape latency of model group was prolonged (P0.05), the number of crossing platform was decreased (P0.05), the difference was statistically significant; compared with model group, The escape latency of rich environment group was shortened (P0.05), the number of crossing platform increased (P0.05), the difference was statistically significant. 2. The expression of BDNF protein in the model group was significantly higher than that in the sham-operated group (P 0.05), and the expression of BDNF protein was significantly higher in the model group than in the sham operation group (P 0.05), and the expression of BDNF protein in the hippocampus of the model group was significantly higher than that in the sham operation group (P 0.05). The positive expression of BDNF protein in hippocampal CA 1 region was more obvious in the rich environment group than in the control group, and the difference was statistically significant (P0.05). 3. The positive expression of apoptotic cells in the model group was significantly higher than that in the sham-operated group at the 28th day after operation (P0.05); compared with the model group, the positive expression of apoptotic cells in the model group was significantly higher than that in the control group (P0.05). The positive expression of apoptotic cells decreased significantly in the rich environment group (P0.05). Conclusion 1. Rich environment can improve learning and memory ability of HIBD rats. 2. 2. Rich environment could up-regulate the expression of BDNF protein in CA 1 area of hippocampus of HIBD rats and down-regulate the expression of apoptotic cells in CA1 area of hippocampus of HIBD rats.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R742

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