本文選題：迷走神經(jīng)刺激 + 海馬　； 參考：《遼寧醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的 觀察迷走神經(jīng)刺激（vagus nerve stimulation，VNS）對海人酸（kainic acid，KA）致癲癇大鼠海馬星形膠質(zhì)細胞縫隙連接蛋白43（connexin43，Cx43）表達的影響，探討Cx43與癲癇之間的關(guān)系及其在VNS治療癲癇中的作用。 方法 將SD大鼠隨機分為對照組、KA組和VNS+KA組，每組10只；KA組和VNS+KA組大鼠左側(cè)腦室注射3μl0.05%KA溶液，制備癲癇模型，對照組注射等量生理鹽水；VNS+KA組行VNS。觀察各組大鼠行為學(xué)變化，記錄皮層腦電圖（electrocorticogram，ECoG）；應(yīng)用免疫組化染色方法和western blot技術(shù)檢測各組大鼠海馬星形膠質(zhì)細胞Cx43的表達。 結(jié)果 大鼠左側(cè)腦室注射KA3～5min后，KA組大鼠為Racine Ⅳ～Ⅴ級重型癲癇發(fā)作，ECoG可見棘波、棘慢波及簇狀高尖波等異常腦電發(fā)放；VNS+KA組大鼠為Racine I～Ⅲ級輕型發(fā)作，腦電發(fā)放較KA組減輕，對照組無癲癇發(fā)作及異常腦電發(fā)放。KA組大鼠海馬Cx43陽性細胞數(shù)和western blot相對表達量值均明顯高于對照組（P＜0.01），VNS+KA組大鼠海馬Cx43陽性細胞數(shù)和western blot相對表達量值明顯低于KA組（P＜0.01）。 結(jié)論 VNS可抑制KA致癇大鼠癲癇發(fā)作，，降低癲癇大鼠海馬Cx43的高表達。提示癲癇大鼠海馬Cx43的表達與癲癇發(fā)作有關(guān)，降低海馬Cx43的表達可能是VNS抑制癲癇發(fā)作的機制之一。
[Abstract]:Objective to observe the effect of vagal nerve stimulation (vagus nerve) on the expression of connexin 43 (connexin 43) in hippocampal astrocytes of epileptic rats induced by kainic acid, and to explore the relationship between Cx43 and epilepsy and its role in the treatment of epilepsy. Methods Sprague-Dawley rats were randomly divided into control group (KA) and VNS Ka group (n = 10). Rats in each group were injected with 3 渭 l 0.05 Ka solution in the left ventricle to make epilepsy model. The control group was injected with the same amount of normal saline and the VNS Ka group was given VNSs. The changes of behavior were observed and electrocortical electroencephalogram (ECoG) was recorded and the expression of Cx43 in hippocampal astrocytes was detected by immunohistochemical staining and western blot technique. Results five minutes after intracerebroventricular injection of KA3, the rats in the Ka group were marked with racine 鈪

本文編號：2092275