本文選題：HU-308 + CB2受體激動劑�。� 參考：《山西醫(yī)科大學》2014年碩士論文

【摘要】：目的 用不同濃度的乙醇干預小膠質(zhì)細胞，選擇出可以使小膠質(zhì)細胞損傷又不至于大量致死的乙醇濃度；探討大麻素II型受體激動劑HU-308對乙醇所致小膠質(zhì)細胞分泌白細胞介素-6（IL-6）的影響。 方法 以離體小鼠的小膠質(zhì)細胞作為研究對象，，分別用(0.00％，0.025％，0.050％，0.1％，0.2％)濃度的乙醇干預小膠質(zhì)細胞72小時，用酶聯(lián)免疫吸附法（Elisa法）檢測IL-6水平。經(jīng)過實驗、統(tǒng)計學分析，選擇0.050％濃度的乙醇用于后續(xù)實驗。 將六孔板中離體的小鼠小膠質(zhì)細胞培養(yǎng)至80％滿瓶底時，分別加入0.050％濃度的乙醇干預，混勻。再將其隨機的分為對照組、1umol/LHU-308干預組、5umol/LHU-308干預組、10umol/LHU-308干預組。依次用1umol/L、5umol/L、10umol/LHU-308干預72小時。用Elisa法檢測IL-6水平。經(jīng)過試驗、統(tǒng)計學分析，得出結(jié)論。 結(jié)果 0.00％乙醇組與0.025％乙醇組比較差別無統(tǒng)計學意義，與0.050％乙醇組、0.10％乙醇組比較差別均有統(tǒng)計學意義，0.025％乙醇組與0.050％乙醇組、0.10％乙醇組比較差別均有統(tǒng)計學意義，0.050％乙醇組與0.10％乙醇組比較差別無統(tǒng)計學意義。 對照組、1umol/LHU-308干預組、5umol/LHU-308干預組、10umol/LHU-308干預組IL-6濃度的均數(shù)值依次降低。對照組與1umol/LHU-308干預組、5umol/LHU-308干預組、10umol/LHU-308干預組比較差別均有統(tǒng)計學意義，1umol/LHU-308干預組與5umol/LHU-308干預組、10umol/LHU-308干預組比較差別均有統(tǒng)計學意義，5umol/LHU-308干預組與10umol/LHU-308干預組比較差別無統(tǒng)計學意義。 結(jié)論 0.025％的乙醇對小膠質(zhì)細胞分泌IL-6水平無明顯影響。0.20％的乙醇則使大部分小膠質(zhì)細胞死亡。0.050％乙醇與0.10％乙醇處理小膠質(zhì)細胞，IL-6分泌水平無明顯差別且均高于對小膠質(zhì)細胞產(chǎn)生炎性損傷的水平，故選用0.050％乙醇濃度作為后續(xù)實驗。 HU-308可以降低乙醇所致的小膠質(zhì)細胞分泌IL-6的水平，且5umol/LHU-308干預與10umol/LHU-308干預無明顯差別。
[Abstract]:objective
The effects of II receptor agonist HU-308 on the secretion of interleukin -6 (IL-6) in microglia induced by ethanol were investigated by interfering with different concentrations of ethanol in microglia.
Method
The microglia in isolated mice was used as the study object. The microglia was treated with ethanol (0%, 0.025%, 0.050%, 0.1%, 0.2%) for 72 hours respectively. The IL-6 level was detected by enzyme linked immunosorbent assay (Elisa method). After the experiment and statistical analysis, the ethanol of 0.050% concentration was selected for the follow-up experiment.
When the isolated mouse microglia in the six pore plate was cultured to the bottom of the 80% full bottle, 0.050% concentration of ethanol was added to the control group. Then it was randomly divided into control group, 1umol/LHU-308 intervention group, 5umol/LHU-308 intervention group and 10umol/LHU-308 intervention group. 1umol/L, 5umol /L, 10umol/LHU-308 intervention for 72 hours. IL- was used to detect IL- by Elisa method. The 6 level. Through the test, statistical analysis, draw the conclusion.
Result
There was no significant difference in the difference between the 0% ethanol group and the 0.025% ethanol group. Compared with the 0.050% ethanol group and the 0.10% ethanol group, there were significant differences in the difference between the 0.025% ethanol group and the 0.050% ethanol group and the 0.10% ethanol group. There was no significant difference in the difference between the 0.050% ethanol group and the 0.10% ethanol group.
The control group, the 1umol/LHU-308 intervention group, the 5umol/LHU-308 intervention group and the 10umol/LHU-308 intervention group decreased the IL-6 concentration in turn. The control group and the 1umol/LHU-308 intervention group, the 5umol/LHU-308 intervention group, the 10umol/LHU-308 intervention group had statistical significance, 1umol/LHU-308 intervention group and 5umol/LHU-308 intervention group, 10umol/LHU-308 intervention. There was statistically significant difference between the two groups. There was no significant difference between the 5umol/LHU-308 intervention group and the 10umol/LHU-308 intervention group.
conclusion
0.025% ethanol had no significant effect on the secretion of IL-6 in microglia and.0.20% ethanol, while most microglia died of.0.050% ethanol and 0.10% ethanol to treat microglia. The level of IL-6 secretion was not significantly different and higher than that of microglia. The concentration of 0.050% ethanol was selected as the post. Continue the experiment.
HU-308 could reduce the level of IL-6 secreted by microglia induced by ethanol, and there was no significant difference between 5umol/LHU-308 intervention and 10umol/LHU-308 intervention.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R741

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