本文選題：骨髓間充質(zhì)干細(xì)胞 + 電針　； 參考：《瀘州醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的通過骨髓間充質(zhì)干細(xì)胞(bone marrow mesenchymal stem cells, BMSCs)移植聯(lián)合電針(electric acupuncture, Ea)刺激治療腦出血大鼠,觀察出血灶及周邊骨形態(tài)發(fā)生蛋白-2(bone morphogenetic protein-2, BMP2)、神經(jīng)膠質(zhì)抗原-2(neuroglia antigen-2, NG2)、半乳糖腦苷脂(galactocerebroside, GALC)、CD68蛋白及層粘連蛋白(laminin, LN)的表達(dá)變化,探討聯(lián)合治療對骨形態(tài)發(fā)生蛋白、腦內(nèi)膠質(zhì)細(xì)胞和血管再生影響。方法100只雄性SD大鼠建立腦出血模型,隨機(jī)分BMSCs移植聯(lián)合電針治療組(BMSCs+Ea組)、MSCs移植組(BMSCs組)、電針治療組(Ea組)和生理鹽水組(NS組),每組25只。術(shù)后第三天分別給予BMSCs移植聯(lián)合電針刺激、BMSCs移植、電針刺激和注射生理鹽水治療；又根據(jù)處死時間的不同,每組大鼠又分成1d、3d、5d、7d、14d5個亞組,每亞組5只。取腦制成冰凍切片,免疫組化檢測BMP2、NG2、GALC、CD68、LN蛋白的表達(dá)。結(jié)果1.出血灶周邊腦區(qū)BMP2陽性細(xì)胞計數(shù)并分析：各組BMP2陽性細(xì)胞數(shù)先呈上升趨勢而后下降。BMSCs+Ea組比NS組在1d-14d時增多,BMSCs組比NS組在3d-5d升高(P0.05)。Ea組和NS組相比較無顯著差異。BMSCs+Ea組比Ea組在1d、5d和7d時增多,BMSCs組比Ea組在1d和5d時上升(P0.05)。BMSCs+Ea組與BMSCs組相比在1d和14d時上調(diào)(P0.05)。2.出血灶周邊腦區(qū)NG2陽性細(xì)胞計數(shù)并分析結(jié)果顯示：BMSCs+Ea組比NS組在1d-7d時增多,BMSCs組比NS組在5d和14d升高(P0.05)。Ea組與NS組相比差異無統(tǒng)計學(xué)意義。BMSCs+Ea組比Ea組在1d、3d、5d和14d時增多,BMSCs組比Ea組5d和14d時上升(P0.05)。BMSCs+Ea組與BMSCs組相比在1d-5d時上調(diào)(P0.05)。3.出血灶周邊腦區(qū)GALC陽性細(xì)胞計數(shù)后分析,BMSCs+Ea組比NS組在1d-7d時增多,BMSCs組比NS組在1d-7d升高,Ea組比NS組在7d時上升(P0.05)。BMSCs+Ea組比Ea組在1d-5d時增多,BMSCs組比Ea組在5d時上升(P0.05)。BMSCs+Ea組與BMSCs組相比在1d-3d時上調(diào)(P0.05)。4.出血灶周邊腦區(qū)CD68蛋白光密度值變化：BMSCs+Ea組、BMSCs組和Ea組CD68蛋白光密度值先緩慢上升而后逐漸下降,7d時達(dá)到峰值。NS組在1d-14d均呈上升趨勢。BMSCs+Ea組比NS組在1d-14d時增大, BMSCs組比NS組在1d-5d升高,在14d時下降,Ea組比NS組在14d時減小(P0.05)。BMSCs+Ea組比Ea組在1d-7d時增大(P0.05)。BMSCs組比Ea組相比較差異無統(tǒng)計學(xué)意義。BMSCs+Ea組與BMSCs組相比在5d-7d時上調(diào)(P0.05)。5.對出血灶周邊腦區(qū)血管計數(shù)并分析：BMSCs+Ea組血管數(shù)比NS組在5d-14d時增多,BMSCs組比NS組在3d-7d升高,Ea組比NS組在3d時上升(P0.05)。BMSCs+Ea組比Ea組在5d-14d時增多,BMSCs組比Ea組5d-14d時上升(P0.05)。BMSCs+Ea組與BMSCs組相比較在7d時上調(diào)(P0.05)。結(jié)論1.BMSCs移植聯(lián)合電針治療較單純的BMSCs移植和電針刺激治療更能促進(jìn)腦出血大鼠BMP2的表達(dá),這有助于腦內(nèi)NSCs和BMSCs向神經(jīng)細(xì)胞方向轉(zhuǎn)化及腦組織結(jié)構(gòu)重塑。2.BMSCs移植聯(lián)合電針治療早期可促進(jìn)少突膠質(zhì)前體細(xì)胞和少突膠質(zhì)細(xì)胞的增殖,利于髓鞘形成；后期則抑制其增殖,可能與防止膠質(zhì)瘢痕的形成有關(guān)。3.BMSCs移植可早期激活小膠質(zhì)細(xì)胞,促進(jìn)其快速增殖；聯(lián)合電針治療后早期上調(diào)其活化和增殖的能力,積極參與免疫反應(yīng)。4.單純BMSCs移植和電針刺激治療可在一定時間內(nèi)促進(jìn)血管再生；BMSCs移植聯(lián)合電針治療更有利于血管的生成,有助于改善出血灶及周邊區(qū)微環(huán)境。
[Abstract]:Objective to treat cerebral hemorrhage rats by transplantation of bone marrow mesenchymal stem cells (BMSCs) and electroacupuncture (electric acupuncture, Ea), and to observe the hemorrhagic foci and peripheral bone morphogenetic protein -2 (bone morphogenetic), glial antigen, and galactoside. The expression of galactocerebroside (GALC), CD68 protein and laminin (laminin, LN) and the effect of combined therapy on bone morphogenetic protein, brain glial cells and vascular regeneration. Methods 100 male SD rats were established with cerebral hemorrhage model, randomly divided into BMSCs graft combined electroacupuncture group (BMSCs+Ea group) and MSCs transplantation group (BMSCs group). Electroacupuncture treatment group (group Ea) and saline group (group NS), 25 in each group, were given BMSCs transplantation combined with electroacupuncture stimulation, BMSCs transplantation, electroacupuncture stimulation and injection of saline for third days after operation, and the rats in each group were divided into 1D, 3D, 5D, 7d, 14d5 subgroups and 5 in each subgroup according to the time of death. The brain was made into frozen section and immune group. Results 1. the expression of BMP2, NG2, GALC, CD68, LN protein. Results the number of BMP2 positive cells in the peripheral brain region of the hemorrhagic focus and analysis: the number of BMP2 positive cells in each group first increased and then decreased in the.BMSCs+Ea group than in the NS group at 1d-14d, and there was no significant difference in the BMSCs group compared with the NS group. The increase of 1D, 5D and 7d, the increase of BMSCs group than that of Ea in 1D and 5D (P0.05).BMSCs+Ea group compared with BMSCs group in 1D and 14d. The.BMSCs+Ea group was more than the Ea group at 1D, 3D, 5D and 14d, and the BMSCs group was higher than the Ea group 5D and 14d. The increase of (P0.05).BMSCs+Ea group was more than that of the Ea group at 1d-5d, and the BMSCs group was higher than that of the Ea group at 5D (P0.05) in the.BMSCs+Ea group compared with the BMSCs group. Group S increased in the 1d-14d group than in the NS group at 1d-14d, and the BMSCs group was higher than the NS group in 1d-5d, and decreased at 14d, and the Ea group was smaller than the NS group in 14d. The number of blood vessels in the peripheral brain region of the hemorrhagic foci was increased by.5.. The number of blood vessels in the group BMSCs+Ea was higher than that in the NS group at 5d-14d, and the BMSCs group was higher than the NS group in 3d-7d, and the Ea group was higher than the NS group at 3D. 1.BMSCs transplantation combined with electroacupuncture can promote the expression of BMP2 in rats with intracerebral hemorrhage more than simple BMSCs transplantation and electroacupuncture stimulation, which helps to transform NSCs and BMSCs into neurons in the brain and to reshape the structure of brain tissue and remodeling.2.BMSCs transplantation combined with electroacupuncture at the early stage to promote the increase of oligodendrocytes and oligodendrocytes. Colonization is beneficial to the formation of myelin sheath; later, it inhibits its proliferation. It may be related to the prevention of the formation of glial scar..3.BMSCs transplantation can activate microglia early and promote its rapid proliferation. The ability to increase its activation and proliferation early after electroacupuncture treatment, and actively participate in the immune response.4. simple BMSCs transplantation and electroacupuncture stimulation treatment may be certain. BMSCs transplantation combined with electro acupuncture treatment is more conducive to angiogenesis and helps to improve the microenvironment of bleeding foci and surrounding areas.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.34

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 辛志成;周政;楊輝;劉俊;何家全;宋業(yè)純;嚴(yán)稽文;;SVZa神經(jīng)干細(xì)胞誘導(dǎo)分化為多巴胺能神經(jīng)元的實驗研究[J];重慶醫(yī)學(xué);2008年05期

2 張圓;楊靜;陽浩;陳鵬慧;;少突膠質(zhì)前體細(xì)胞在髓鞘再生中作用的研究進(jìn)展[J];重慶醫(yī)學(xué);2010年17期

3 陳錦華;楊輝;尹昌林;張治元;劉仕勇;何家全;高方友;;BMP2在SVZa神經(jīng)干細(xì)胞向GABA能神經(jīng)元分化中的調(diào)控作用[J];第三軍醫(yī)大學(xué)學(xué)報;2007年08期

4 陳鵬慧;蔡文琴;王麗雁;吳喜貴;;NG2膠質(zhì)細(xì)胞在成年大鼠腦內(nèi)的分布及形態(tài)特征[J];第三軍醫(yī)大學(xué)學(xué)報;2008年01期

5 馬秀華;呂富榮;呂發(fā)金;黃鈾新;肖智博;肖云華;楊柳;張瑜;;影像學(xué)對骨髓間質(zhì)干細(xì)胞治療腦卒中實驗效果的評價[J];第三軍醫(yī)大學(xué)學(xué)報;2009年17期

6 繆星宇;劉曉斌;岳青;秋楠;黃衛(wèi)東;王繼軍;許彥鋼;張越林;楊軍;陳新林;;去鐵胺對大鼠腦出血后小膠質(zhì)細(xì)胞活化的抑制及其繼發(fā)性神經(jīng)損傷的保護(hù)作用[J];南方醫(yī)科大學(xué)學(xué)報;2012年07期

7 周金玲;王瑩;劉宏斌;劉曼華;;巨噬細(xì)胞CD68與子宮腺肌病發(fā)病機(jī)制的關(guān)系[J];中國婦產(chǎn)科臨床雜志;2013年03期

8 黃月;許予明;宋波;方樹友;;骨髓間充質(zhì)干細(xì)胞移植對缺血再灌注大鼠腦神經(jīng)細(xì)胞凋亡及Bcl-2、Bax蛋白表達(dá)的影響[J];鄭州大學(xué)學(xué)報(醫(yī)學(xué)版);2006年05期

9 李瑞梅;楊迎春;任占川;;大鼠腦缺血再灌注后層粘連蛋白表達(dá)的研究[J];中外醫(yī)療;2011年14期

10 楊成,劉同慎,吳洪華,劉文波;電針對脊髓損傷后少突膠質(zhì)細(xì)胞再髓鞘化的影響[J];解剖學(xué)雜志;2005年04期

，

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