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  本文選題：脂多糖 + Notch信號通路�。� 參考：《神經(jīng)解剖學(xué)雜志》2017年05期

【摘要】：目的:探討Notch信號通路在激活的BV-2小膠質(zhì)細(xì)胞的表達(dá)及依達(dá)拉奉對其表達(dá)的影響。方法:體外培養(yǎng)BV-2小膠質(zhì)細(xì)胞系,將其分為對照組、脂多糖(lipopolysaccharide,LPS)激活處理3 h組和依達(dá)拉奉預(yù)處理1 h+LPS激活3 h組,采用免疫熒光雙標(biāo)染色及蛋白質(zhì)印跡法檢測Notch信號通路相關(guān)蛋白在激活的BV-2小膠質(zhì)細(xì)胞的表達(dá)變化,包括Notch-1及下游的細(xì)胞內(nèi)Notch受體結(jié)構(gòu)域(intracellular Notch receptor domain,NICD)、重組結(jié)合蛋白抑制子(recombining binding protein suppressor of hairless,RBP-JK)、轉(zhuǎn)移因子發(fā)卡和split-1增強(qiáng)子(transcription factor hairy and enhancer of split-1,Hes-1),以及依達(dá)拉奉干預(yù)后的影響。結(jié)果:免疫熒光雙標(biāo)結(jié)果顯示,LPS激活的BV-2小膠質(zhì)細(xì)胞Notch信號通路相關(guān)蛋白(Notch1、NICD、RBP-JK、Hes-1)熒光表達(dá)出現(xiàn)明顯增強(qiáng),依達(dá)拉奉可顯著減少激活的BV-2小膠質(zhì)細(xì)胞上述蛋白的熒光表達(dá)。Western Blot結(jié)果也顯示依達(dá)拉奉可明顯抑制激活的小膠質(zhì)細(xì)胞Notch1、NICD、RBP-JK、Hes1的蛋白表達(dá),結(jié)果具有統(tǒng)計(jì)學(xué)意義。結(jié)論:依達(dá)拉奉可通過Notch信號通路調(diào)節(jié)小膠質(zhì)細(xì)胞的激活,從而影響其功能活動。
[Abstract]:Aim: to investigate the expression of Notch signaling pathway in activated BV-2 microglia and the effect of Edaravone on its expression. Methods: BV-2 microglia cell lines were cultured in vitro and divided into control group, lipopolysaccharide (LPS) activation treatment group for 3 h and Edaravone pretreatment 1 h LPS activation group for 3 h. The expression of Notch signaling pathway related proteins in activated BV-2 microglia was detected by double immunofluorescence staining and Western blotting. These include Notch-1 and its downstream intracellular Notch receptor domain, recombining binding protein suppressor of hairless RBP-JKN, transcription factor hairy and enhancer of split-1hes-1hes-1s, and the effect of Edaravone on it. Results: the results of double immunofluorescence showed that the fluorescence expression of LPS-activated BV-2 microglia Notch signal pathway related protein (NICDP-JKP- Hes-1) was significantly increased. Edaravone significantly reduced the fluorescence expression of the above mentioned protein in activated BV-2 microglia. Western Blot results also showed that Edaravone could significantly inhibit the expression of Notch1NICDP-JKHes1 protein in activated microglia cells, and the results were statistically significant. Conclusion: Edaravone can regulate the activation of microglia through Notch signaling pathway and affect its function.
【作者單位】： 昆明醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院人體解剖學(xué)與組織胚胎學(xué)系;昆明醫(yī)科大學(xué)科研實(shí)驗(yàn)中心;昆明醫(yī)科大學(xué)海源學(xué)院;
【基金】：云南省應(yīng)用基礎(chǔ)研究計(jì)劃重點(diǎn)項(xiàng)目(2015FA020) 國家自然科學(xué)基金(31260254) 云南省教育廳科學(xué)研究基金項(xiàng)目(2015C015Y)
【分類號】：R741

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