發(fā)布時間：2018-05-27 15:08

  本文選題：腦梗死 + 模型　； 參考：《山西醫(yī)科大學》2016年碩士論文

【摘要】：第一部分目的:選取合適線栓直徑,建立符合后續(xù)實驗研究需要的實驗模型。方法:選取體重250±20g健康雄性SD大鼠20只,隨機分為A、B兩組(n=10),實驗操作采用改良線栓法造成大鼠右側(cè)大腦中動脈閉塞(MCAO)模型,A組線栓直徑0.26mm,B組0.30mm,于6小時后拔出線栓再灌注,24小時后行Longa神經(jīng)功能缺損評分及TTC法測腦梗死體積并計算百分比。結(jié)果描述主要以sx±表示,采用t檢驗及卡方檢驗,P0.05認為差異具有統(tǒng)計學意義。結(jié)果:1.兩組大鼠缺血再灌注后均表現(xiàn)出神經(jīng)功能缺損:走路時一側(cè)行走不利,身體傾斜向一側(cè)或原地劃圈打轉(zhuǎn),精神低靡,活動較前明顯減少,自主飲食攝水減少。Longa評分A組(2.4±0.69)分,B組(3.1±0.73)分,存在明顯統(tǒng)計學差異(P0.05)。2.A組10只大鼠缺血再灌注后全部存活,B組3只大鼠表現(xiàn)為意識喪失,1只死亡,1-3分表明造模成功,經(jīng)卡方檢驗,兩組之間造模成功率A組高于B組。3.腦切片TTC染色后可見正常腦組織部分呈紅色,而梗死區(qū)域呈現(xiàn)白色。A組(35.89±3.33)%,B組(39.69±4.44)%,結(jié)果顯示:兩組大鼠腦梗死體積百分比A組小于B組。結(jié)論:體重250±20g大鼠線栓法缺血6小時再灌注造模時,選用直徑0.26mm的魚線所造模型成功率更高,可以造成穩(wěn)定的局灶性腦梗死,為后續(xù)實驗的順利進行提供一定的模型基礎(chǔ)。第二部分目的:探討人重組粒細胞集落刺激因子(rh G-CSF)對大鼠腦缺血超時間窗(缺血6小時)溶栓治療后基質(zhì)金屬蛋白酶-9(MMP-9)表達及并發(fā)癥出血轉(zhuǎn)化發(fā)生的影響,為擴大溶栓時間窗提供理論依據(jù)。方法:選取健康雄性SD大鼠36只,按第一部分制備MCAO模型,隨機分為3組,即空白對照組、重組組織型纖溶酶原激活劑(rt-PA)組及聯(lián)合治療組(n=12),缺血后6h三組給予拔除線栓處理,同時給藥,rt-PA組給予rt-PA 10 mg/kg,聯(lián)合治療組給予rt-PA(10 mg/kg)+rh G-CSF(300μg/kg),空白對照組僅給等容積生理鹽水。再灌注24h后行神經(jīng)功能缺損評分,免疫組化法測定MMP-9表達,光譜光度法測量腦出血量。統(tǒng)計描述主要以sx±表示,定量資料采用t檢驗和方差分析,定性資料采用卡方檢驗。P0.05認為差異具有統(tǒng)計學意義。結(jié)果:1.三組大鼠造模后均表現(xiàn)出神經(jīng)功能缺損,分別給予各組處理后神經(jīng)功能缺損評分空白對照組(2.25±0.622)分,rt-PA組(2.08±0.515)分,聯(lián)合治療組(1.33±0.651)分,經(jīng)LSD-t檢驗,結(jié)果顯示:聯(lián)合治療組與其他兩組的Longa評分值均有明顯的統(tǒng)計學差異(P0.05)。2.MMP-9表達:非缺血側(cè)半球罕見MMP-9免疫陽性細胞表達,缺血半球側(cè)則表達較多,呈棕黃色淡染。各組缺血側(cè)與正常對側(cè)情況比較結(jié)果表明:三組大鼠病變側(cè)MMP-9表達高于正常對側(cè)。缺血側(cè)陽性細胞計數(shù)空白對照組24.33±2.733,rt-PA組30.00±2.280,聯(lián)合治療組20.83±1.722,LSD-t檢驗結(jié)果表明:rt-PA組MMP-9表達較空白組明顯增加,聯(lián)合治療組MMP-9表達較rt-PA組下降。3.三組大鼠腦出血量比較結(jié)果顯示:rt-PA組腦出血量較空白對照組明顯增加,而聯(lián)合治療組較rt-PA組出血量明顯減少(P0.05)。結(jié)論:rh G-CSF降低大鼠超時間窗(6h)溶栓后出血轉(zhuǎn)化的發(fā)生,可能與rh G-CSF降低MMP-9表達有關(guān)。
[Abstract]:The first part was to select the appropriate diameter of the thread and establish the experimental model in accordance with the needs of the follow-up experiment. Methods: 20 rats with weight 250 20g healthy male SD rats were selected and randomly divided into A and B two groups (n=10). The experimental operation by modified thread embolus was used to cause the right middle cerebral artery occlusion (MCAO) model in rats, the diameter of the A group, the B group 0.30mm, and the A group. 6 hours after pulling out the thread thrombus and reperfusion, after 24 hours, the Longa nerve function defect score and the TTC method were used to measure the cerebral infarction volume and calculate the percentage. The results were described mainly by SX +, using t test and chi square test, P0.05 thought the difference was statistically significant. Results: 1. two groups of rats showed nerve function defect after blood reperfusion: walking On one side, the body was disadvantageous, the body sloped to one side or the original area, the spirit was low, the activity was reduced obviously, the.Longa score in A group (2.4 + 0.69) and B group (3.1 + 0.73) were reduced, and there was significant statistical difference (P0.05) in group.2.A, 10 rats were all survived after ischemia and reperfusion, and 3 rats in group B showed loss of consciousness, 1 Only death, 1-3 points showed the success of the model, after the chi square test, the success rate of the two groups was higher than the B group.3. brain slice TTC staining, the normal brain tissue was red, and the infarct area showed white.A group (35.89 + 3.33)%, B group (39.69 + 4.44)%. The results showed that the percentage of cerebral infarction volume A group in two groups was less than that of group B. Conclusion: weight 250 in group two. Conclusion: weight 250. Conclusion: body weight 250. Conclusion: weight 250 in group two. Conclusion: body weight 250 is less than that of group B. Conclusion: weight 250 of group of cerebral infarction in two groups is less than that of group B. Conclusion: weight 250 of group.3. is less than that of group B. Conclusion: weight 250 in group of two rats is smaller than that of group B. Conclusion: weight 250 of group.3. is less than that of group B. Conclusion: weight 250 In the 6 hour reperfusion model of 6 hour ischemia in the rats of + 0.26mm, the success rate of the model with the diameter of 0.26mm is higher, which can cause a stable focal cerebral infarction and provide a certain model basis for the smooth progress of the follow-up experiment. The purpose of this study is to explore the human recombinant granulocyte colony-stimulating factor (RH G-CSF) for the cerebral ischemia in rats The effect of time window (ischemia 6 hours) on the expression of matrix metalloproteinase -9 (MMP-9) and complication of hemorrhage transformation after thrombolytic therapy, in order to provide a theoretical basis for expanding the thrombolytic time window. Methods: 36 healthy male SD rats were selected and the MCAO model was prepared in the first part, which were randomly divided into 3 groups, that is, the blank control group and the recombinant tissue type plasminogen activator. The rt-PA group and the combined treatment group (n=12), after the ischemia, the 6h three groups were treated with the extraction line thrombus, at the same time, the drug was given, the rt-PA group was given rt-PA 10 mg/kg, the combined treatment group was given rt-PA (10 mg/kg) +rh G-CSF (300 mu g/kg), the blank control group was only given equal volume physiological saline. The amount of cerebral hemorrhage was measured by spectral photometric method. The statistical description was mainly expressed in SX. The quantitative data were analyzed by t test and variance analysis. The qualitative data were statistically significant by chi square test.P0.05. Results: 1. the three groups of rats showed nerve function defect after the model was made, and the scores of nerve function defect were given to each group respectively. The group (2.25 + 0.622), rt-PA group (2.08 + 0.515), combined treatment group (1.33 + 0.651) score and LSD-t test showed that the Longa score of the combined treatment group and the other two groups had significant statistical difference (P0.05).2.MMP-9 expression: the expression of MMP-9 immunoreactive cells in the non ischemic hemisphere was rare, and the ischemic hemisphere side was more expressed in brown. The comparison between the ischemic side and the normal contralateral situation showed that the expression of MMP-9 in the three groups was higher than that in the normal contralateral side. The positive cells in the ischemic side were 24.33 + 2.733 in the blank control group, 30 + 2.280 in the group rt-PA and 20.83 in the combined treatment group. The results of LSD-t test showed that the expression of MMP-9 in the group rt-PA was significantly higher than that in the blank group. The combination of the MMP-9 expression in the group rt-PA was significantly higher than that in the blank group. Compared with group rt-PA, the comparison of MMP-9 expression in the group of.3. three rats showed that the amount of cerebral hemorrhage in group rt-PA was significantly higher than that in the blank control group, while the amount of hemorrhage in the combined treatment group was significantly lower than that in the rt-PA group (P0.05). Conclusion: RH G-CSF reduced the occurrence of hemorrhage after thrombolytic thrombolysis in the super time window (6h) of rats, and may reduce the MMP-9 table with RH G-CSF. It's up to it.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R743.3

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