本文選題：miR-b + 環(huán)氧化酶-�。� 參考：《中南大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年02期

【摘要】：目的:觀察不同級別神經(jīng)膠質(zhì)瘤中miR-26b和環(huán)氧化酶(COX)-2的表達情況,研究miR-26b對于神經(jīng)膠質(zhì)瘤細胞增殖、侵襲和遷移的影響。方法:運用Western印跡和實時定量熒光PCR(q RT-PCR)檢測不同級別神經(jīng)膠質(zhì)瘤中miR-26b和COX-2的表達情況;使用miR-26b mimic轉(zhuǎn)染人神經(jīng)膠質(zhì)瘤細胞U87,上調(diào)miR-26b的表達;q RT-PCR檢測miR-26b mimic轉(zhuǎn)染后miR-26b和COX-2 mRNA的表達變化情況;雙熒光素酶報告基因系統(tǒng)檢測miR-26b對COX-2轉(zhuǎn)錄活性的影響;使用Transwell侵襲實驗檢測miR-26b對人神經(jīng)膠質(zhì)瘤細胞U87侵襲能力的影響;使用劃痕實驗檢測miR-26b對人神經(jīng)膠質(zhì)瘤細胞U87遷移能力的影響;使用CCK-8(cell counting kit-8)檢測miR-26對人神經(jīng)膠質(zhì)瘤細胞U87增殖能力的影響;裸鼠體內(nèi)成瘤實驗檢測過表達miR-26b后膠質(zhì)瘤細胞成瘤能力的變化。結(jié)果:隨著神經(jīng)膠質(zhì)瘤腫瘤級別的增加,miR-26b表達明顯降低(P0.05),而COX-2明顯增加,差異有統(tǒng)計學(xué)意義(P0.001);雙熒光素酶實驗證實miR-26b可以直接靶向調(diào)控COX-2的蛋白表達水平;使用miR-26b mimic明顯上調(diào)miR-26b的表達(P0.05);上調(diào)miR-26b可以顯著降低COX-2的表達(P0.05);上調(diào)miR-26b可以抑制神經(jīng)膠質(zhì)瘤細胞增殖、侵襲和遷移能力(P0.05)。實驗組和對照組相比腫瘤的質(zhì)量和體積都變小。結(jié)論:隨著神經(jīng)膠質(zhì)瘤腫瘤級別的增加,miR-26b表達逐漸降低,而COX-2表達逐漸增加。miR-26b可通過抑制COX-2表達從而抑制神經(jīng)膠質(zhì)瘤的增殖、侵襲和遷移。
[Abstract]:Aim: to observe the expression of miR-26b and cyclooxygenase COX-2 in gliomas and to investigate the effects of miR-26b on the proliferation, invasion and migration of glioma cells. Methods: the expression of miR-26b and COX-2 in gliomas of different grades were detected by Western blot and real-time fluorescence PCR(q RT-PCR. MiR-26b mimic transfected human glioma cell line U87 was used to up-regulate the expression of miR-26b and detect the changes of miR-26b and COX-2 mRNA expression after miR-26b mimic transfection, and the effect of miR-26b on COX-2 transcription activity was detected by double luciferase reporter gene system. Transwell invasion assay was used to detect the effect of miR-26b on the invasion ability of human glioma cell line U87, and scratch test was used to detect the effect of miR-26b on the migration ability of human glioma cell line U87. CCK-8(cell counting kit-8) was used to detect the effect of miR-26 on the proliferation of human glioma cell line U87, and the tumorigenic ability of human glioma cell line U87 was detected by tumorigenic assay in nude mice after the expression of miR-26b. Results: with the increase of tumor grade of glioma, the expression of miR-26b decreased significantly, while the expression of COX-2 increased significantly (P 0.001). Double luciferase assay confirmed that miR-26b could directly target the expression of COX-2 protein. The expression of miR-26b was significantly up-regulated by miR-26b mimic, the expression of COX-2 was significantly decreased by upregulation of miR-26b, and the proliferation, invasion and migration of glioma cells was inhibited by up-regulation of miR-26b. The mass and volume of the tumor in the experimental group were smaller than that in the control group. Conclusion: with the increase of glioma grade, the expression of miR-26b decreases gradually, while the expression of COX-2 increases gradually. MiR-26b inhibits the proliferation, invasion and migration of gliomas by inhibiting the expression of COX-2.
【作者單位】： 海南醫(yī)學(xué)院第一附屬醫(yī)院神經(jīng)外科;中山大學(xué)孫逸仙紀(jì)念醫(yī)院神經(jīng)外科;
【基金】：海南省自然科學(xué)基金(20168298)~~
【分類號】：R739.41

【相似文獻】

相關(guān)期刊論文 前10條

1 陳贊,楊立莊,葉偉,王伊龍,趙鵬,李永利,康軍,鄭永日,曹陽;神經(jīng)膠質(zhì)瘤細胞熱休克蛋白抗原肽復(fù)合物的研究[J];中華實驗外科雜志;2003年05期

2 陳贊 ,楊立莊 ,葉偉;神經(jīng)膠質(zhì)瘤細胞熱休克蛋白抗原肽復(fù)合物的研究[J];哈爾濱醫(yī)科大學(xué)學(xué)報;2003年05期

3 張玲華;;神經(jīng)膠質(zhì)瘤的診斷與治療現(xiàn)狀[J];實用醫(yī)技雜志;2007年24期

4 周文光;李南華;王廷芳;;鼻內(nèi)型神經(jīng)膠質(zhì)瘤一例[J];臨床耳鼻咽喉頭頸外科雜志;1990年02期

5 ;顱面神經(jīng)膠質(zhì)瘤[J];國外醫(yī)學(xué).耳鼻咽喉科學(xué)分冊;1997年02期

6 薛德麟,雷霆;要重視神經(jīng)膠質(zhì)瘤的治療[J];中國臨床神經(jīng)外科雜志;2002年01期

7 肖敏 ,吳淼 ,曾冬竹 ,饒蕓;樹突狀細胞瘤苗誘導(dǎo)的抗神經(jīng)膠質(zhì)瘤作用體外實驗研究[J];現(xiàn)代醫(yī)藥衛(wèi)生;2003年01期

8 戴捷,于\，

本文編號：1927114