本文選題：受體 + 阿片樣��； 參考：《中國神經(jīng)精神疾病雜志》2017年08期

【摘要】：目的觀察δ阿片受體激動劑DADLE對大鼠全腦缺血再灌注后神經(jīng)元凋亡細(xì)胞及磷酸化p38表達(dá)的影響,探討DADLE在全腦缺血再灌注損傷細(xì)胞凋亡中的腦保護(hù)機(jī)制。方法采用二血管加低血壓阻斷法建立大鼠全腦缺血再灌注模型,隨機(jī)分為假手術(shù)組(n=10),模型組(n=10),治療組(再按DADLE靜脈注射不同劑量分為3個亞組2 mg組、3 mg組、5 mg組,n=10),TUNEL法檢測凋亡細(xì)胞,Western blot檢測神經(jīng)元磷酸化p38絲裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)蛋白的表達(dá)。結(jié)果 DADLE可顯著減少大鼠缺血神經(jīng)元細(xì)胞凋亡,與假手術(shù)組比較,缺血后大鼠神經(jīng)元磷酸化p38 MAPK表達(dá)水平增加(P0.05),與模型組比較,治療組各組磷酸化p38 MAPK表達(dá)水平有所降低(LSD-t值依次為0.61、1.61、1.8,P0.05),且其作用呈劑量依賴性。結(jié)論δ阿片受體激動劑DADLE對大鼠全腦缺血再灌注損傷有神經(jīng)保護(hù)作用,抑制磷酸化p38 MAPK的表達(dá),減少細(xì)胞凋亡是其腦保護(hù)作用機(jī)制之一。
[Abstract]:Objective to investigate the effects of 未 opioid receptor agonist (DADLE) on neuronal apoptosis and phosphorylated p38 expression after global cerebral ischemia-reperfusion in rats, and to explore the brain protective mechanism of DADLE during global cerebral ischemia-reperfusion injury. Methods the rat model of global cerebral ischemia-reperfusion was established by two-vessel and hypotension occlusion. They were randomly divided into sham-operation group and model group. The treatment group was divided into three subgroups according to different doses of DADLE intravenously. The treatment group was divided into 3 subgroups (2 mg group, 3 mg group, 5 mg group). Western blot was used to detect phosphorylated p38 mitogen-activated protein (p38 mitogen activated protein) in neurons of apoptotic cells. Expression of mitogen activated protein kinase- MAPK protein. Results compared with sham operation group, the expression of phosphorylated p38 MAPK increased significantly in DADLE group, and compared with model group, the expression level of phosphorylated p38 MAPK increased significantly in DADLE group. The expression of phosphorylated p38 MAPK in the treatment group was decreased in a dose-dependent manner. Conclusion DADLE, a 未 opioid receptor agonist, has neuroprotective effect on global cerebral ischemia-reperfusion injury in rats, inhibits the expression of phosphorylated p38 MAPK and reduces apoptosis.
【作者單位】： 廣州醫(yī)科大學(xué)附屬第一醫(yī)院急診科;
【基金】：廣州市醫(yī)藥衛(wèi)生科技項目(編號:20141A011078)
【分類號】：R743

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本文編號：1913815