本文選題：偏頭痛 + S100B蛋白�。� 參考：《河北醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:偏頭痛是神經(jīng)內(nèi)科的一種常見疾病,發(fā)病機(jī)制尚不十分清楚。最近的研究提出偏頭痛在反復(fù)發(fā)作的基礎(chǔ)上可能導(dǎo)致神經(jīng)細(xì)胞的損傷,而并不是人們以往認(rèn)為的一種良性功能性頭痛疾病。Sangtec-I00B(S-100B)蛋白和神經(jīng)元特異性烯醇化酶(NSE)作為中樞神經(jīng)系統(tǒng)損傷的標(biāo)志物,可反映神經(jīng)膠質(zhì)細(xì)胞和神經(jīng)元的損傷,目前成為臨床的研究熱點(diǎn)之一并引起廣大學(xué)者的關(guān)注。本研究旨在探討偏頭痛患者血清S100B蛋白、NSE水平及其臨床意義,并分析了血清S100B蛋白、NSE水平與偏頭痛病程、持續(xù)時(shí)間、發(fā)作頻率、視覺模擬量表評(píng)分(VAS)及頭痛影響測(cè)驗(yàn)(HIT-6)評(píng)分的相關(guān)性。以期進(jìn)一步了解偏頭痛的發(fā)病機(jī)制及是否存在腦損傷。方法:1選取2015年3月—2016年12月就診于河北醫(yī)科大學(xué)第二醫(yī)院神經(jīng)內(nèi)科門診的偏頭痛患者53例,其中有先兆偏頭痛患者20例,無(wú)先兆偏頭痛患者33例。采用國(guó)際頭痛疾病分類第三版(Beta版)中偏頭痛診斷標(biāo)準(zhǔn),選取河北醫(yī)科大學(xué)第二醫(yī)院體檢中心的50例健康體檢者為對(duì)照組。并對(duì)其一般資料、臨床資料及流行病學(xué)資料進(jìn)行采集。2在偏頭痛發(fā)作間期采集所有入組病人的靜脈血5 ml,離心后收集上層血清,低溫保存至檢測(cè)。S100B蛋白、NSE均采用酶聯(lián)免疫吸附試驗(yàn)檢測(cè)。3應(yīng)用SPSS20.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析,比較偏頭痛組與健康對(duì)照組間的差異,有先兆偏頭痛組與無(wú)先兆組的差異,男性偏頭痛患者與女性偏頭痛患者的差異,分別分析血清S100B蛋白和NSE與偏頭痛病程、持續(xù)時(shí)間、發(fā)作頻率、VAS評(píng)分及HIT-6評(píng)分的相關(guān)性。結(jié)果:1偏頭痛組血清S100B蛋白明顯高于健康對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義,(P0.01);偏頭痛組血清NSE水平與對(duì)照組差異無(wú)統(tǒng)計(jì)學(xué)意義,(P0.05)。2有先兆和無(wú)先兆偏頭痛組患者血清S100B蛋白差異無(wú)統(tǒng)計(jì)學(xué)意義,CP0.05);有先兆偏頭痛組患者血清NSE水平雖高于無(wú)先兆偏頭痛組患者,但未達(dá)到統(tǒng)計(jì)學(xué)意義,(P0.05)。3在健康對(duì)照組中男性與女性血清S100B蛋白、NSE差異均無(wú)統(tǒng)計(jì)學(xué)意義,(P0.05)。在偏頭痛組中男性與女性患者血清S100B蛋白、NSE差異均無(wú)統(tǒng)計(jì)學(xué)意義,(P0.05)。4偏頭痛組患者血清S100B蛋白、NSE水平與偏頭痛患者的病程、持續(xù)時(shí)間、發(fā)作頻率、VAS評(píng)分及HIT-6評(píng)分均無(wú)顯著相關(guān)性。結(jié)論:本研究結(jié)果表明在偏頭痛發(fā)作間期存在神經(jīng)膠質(zhì)細(xì)胞的激活或損傷,并存在血腦屏障(BBB)的功能障礙;同樣提示在偏頭痛發(fā)作間期并沒有明確的神經(jīng)元損傷。
[Abstract]:Objective: migraine is a common disease in neurology. Recent research suggests that migraine attacks on a recurring basis may lead to nerve cell damage. It is not a benign functional headache disease known as Sangtec-I00BFS-100B) and neuron specific enolase (NSE) as a marker of central nervous system injury, which can reflect the injury of glial cells and neurons. At present, it has become one of the focus of clinical research and attracted the attention of the majority of scholars. The purpose of this study was to investigate the serum S100B protein NSE level and its clinical significance in migraine patients, and to analyze the relationship between serum S100B protein NSE level and migraine duration, duration and frequency of attack. The correlation between visual analogue scale (VAS) and headache impact test (HIT- 6). In order to further understand the pathogenesis of migraine and whether there is brain damage. Methods from March 2015 to December 2016, 53 patients with migraine were selected from the Department of Neurology, second Hospital of Hebei Medical University, including 20 patients with aura migraine and 33 patients without aura migraine. Using the diagnostic criteria of migraine in the third edition of the International Classification of headache Diseases, 50 healthy people in the Center of physical examination of the second Hospital of Hebei Medical University were selected as the control group. The general data, clinical data and epidemiological data were collected. 2 the venous blood was collected from all the patients during the period of migraine attack, and the upper serum was collected after centrifugation. The difference between migraine group and healthy control group was compared between migraine group and healthy control group, and there was difference between aura migraine group and no aura group by enzyme linked immunosorbent assay (Elisa). The differences between male migraine patients and female migraine patients were analyzed. The correlation of serum S100B protein and NSE with migraine duration, frequency of attack and HIT-6 score were analyzed. Results the serum S100B protein in migraine group was significantly higher than that in healthy control group. There was no significant difference in serum NSE level between migraine group and control group. There was no significant difference in serum S100B protein between patients with migraine and without migraine, and NSE with migraine with aura. Although the level of migraine group without aura was higher than that of the patients without aura, However, there was no significant difference in serum S100B protein NSE between male and female in the healthy control group. There was no significant difference in serum S100B protein levels between male and female patients in migraine group. There was no significant correlation between the serum S100B protein level and the course, duration, frequency of attack and HIT-6 score of migraine patients. Conclusion: the results of this study indicate that there is activation or injury of glial cells and BBB dysfunction in migraine interictal period, and there is no definite neuronal injury during migraine interictal period.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R747.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 范凱威;任璐;張茜;王志紅;耿左軍;張陽(yáng);張璐;;有先兆偏頭痛患者發(fā)作間期的靜息態(tài)功能磁共振變化[J];腦與神經(jīng)疾病雜志;2016年02期

2 李佩青;周偉;張U，

本文編號(hào)：1907095