本文選題：S-腺苷蛋氨酸 + 氯化鉀��； 參考：《中國(guó)藥理學(xué)通報(bào)》2017年12期

【摘要】：目的探討S-腺苷蛋氨酸(S-adenosylmethionine,SAM)對(duì)KCl刺激引起的原代培養(yǎng)的小鼠皮層神經(jīng)元損傷是否有保護(hù)作用及機(jī)制。方法將原代培養(yǎng)1周的小鼠皮層神經(jīng)元隨機(jī)分為3組:正常(Control)組、KCl組、SAM-KCl組。用倒置顯微鏡觀察各組細(xì)胞形態(tài)的變化,用q PCR、Western blot分別檢測(cè)各組質(zhì)膜微囊結(jié)構(gòu)蛋白~(-1)(caveolin~(-1),Cav~(-1))mRNA及蛋白的表達(dá)變化。結(jié)果 0.05 mol·L~(-1)KCl作用于原代培養(yǎng)1周的小鼠皮層神經(jīng)元12 h可使神經(jīng)元產(chǎn)生明顯的損傷,突起縮短變細(xì),胞體變小,細(xì)胞聚團(tuán)。0.02 mol·L~(-1)SAM預(yù)處理6 h可明顯降低KCl引起的細(xì)胞損傷,q PCR、Western blot分析顯示KCl可使原代培養(yǎng)1周的皮層神經(jīng)元Cav~(-1) mRNA及蛋白表達(dá)增高(P0.01),SAM可降低KCl引起的神經(jīng)元Cav~(-1) mRNA及蛋白表達(dá)增高(P0.05)。結(jié)論 SAM對(duì)KCl引起的神經(jīng)元損傷有保護(hù)作用,該保護(hù)作用與其降低Cav~(-1)的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the protective effect and mechanism of S-adenosylmethionine (S-adenosylmethionine) on primary cultured cortical neuron injury induced by KCl in mice. Methods the primary cultured cortical neurons were randomly divided into three groups: normal control group (KCl) group and SAM-KCl group. The morphologic changes of the cells in each group were observed by inverted microscope. The mRNA and protein expression of the plasma membrane microencapsulation protein (PMC) were detected by qPCRX Western blot in each group. Results the effects of 0. 05 mol L~(-1)KCl on primary cultured mouse cortical neurons for 12 h resulted in obvious damage to the neurons, the process was shortened and the body of the neurons became smaller. Cell cluster. 0.02 mol L~(-1)SAM pretreatment for 6 h significantly reduced cell damage induced by KCl. Western blot analysis showed that KCl could increase the expression of mRNA and protein in primary cultured cortical neurons for 1 week. Conclusion SAM has a protective effect on the neuronal injury induced by KCl, which is related to the decrease of Cavo 1 expression.
【作者單位】： 徐州醫(yī)科大學(xué)江蘇省麻醉學(xué)重點(diǎn)實(shí)驗(yàn)室江蘇省麻醉與鎮(zhèn)痛應(yīng)用技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No 81500950)
【分類號(hào)】：R741

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