本文選題：STAT3 + β-catenin　； 參考：《蘇州大學(xué)》2014年碩士論文

【摘要】：目的研究STAT3、β-catenin和VEGF在膠質(zhì)瘤組織及正常腦組織中的蛋白表達(dá)，探討三者在人腦膠質(zhì)細(xì)胞瘤發(fā)生、發(fā)展、增殖和浸潤中的作用，分析三者與膠質(zhì)瘤臨床觀察指標(biāo)（性別、年齡、腫瘤發(fā)生部位和膠質(zhì)瘤WHO分級）的關(guān)系以及三者在膠質(zhì)瘤組織中表達(dá)的相關(guān)性，從而指導(dǎo)膠質(zhì)瘤惡性程度的判斷和預(yù)后評估，為尋找膠質(zhì)瘤靶向治療的新靶點提供理論基礎(chǔ)。 方法收集徐州醫(yī)學(xué)院附屬連云港第一人民醫(yī)院病理科2009年1月～2012年12月膠質(zhì)瘤存檔臘塊標(biāo)本106例，均采集于存檔病例。其中，男性62例，女性44例，男女性別比為1.4:1；年齡最小者為11歲，最大者為78歲，平均年齡46.4歲。106例腦膠質(zhì)瘤患者病理資料：所有膠質(zhì)瘤標(biāo)本均經(jīng)神經(jīng)外科手術(shù)切除所得且已經(jīng)病理診斷證實，并由連云港第一人民院病理科診斷醫(yī)師按中樞神經(jīng)系統(tǒng)腫瘤WHO分類最新標(biāo)準(zhǔn)（2007年）分為Ⅰ、Ⅱ、Ⅲ、Ⅳ級。低級別組(Ⅰ、Ⅱ級)41例，高級別組(Ⅲ、Ⅳ級)65例；其中Ⅰ級為13例，Ⅱ級為28例，Ⅲ級為31例，Ⅳ級34例。對照組10例正常腦組織標(biāo)本通過顱內(nèi)減壓手術(shù)獲得。實驗采用免疫組織化學(xué)Envision法檢測STAT3、β-catenin及VEGF在人腦膠質(zhì)瘤組織中的表達(dá)情況，結(jié)合臨床病理資料進(jìn)行統(tǒng)計分析，數(shù)據(jù)結(jié)果應(yīng)用SPSS19.0統(tǒng)計軟件處理，根據(jù)數(shù)據(jù)類型選用秩和檢驗及Spearman相關(guān)分析，以P＜0.05為差異有統(tǒng)計學(xué)意義。 結(jié)果 1.STAT3在正常腦組織中未發(fā)現(xiàn)陽性表達(dá)，在膠質(zhì)瘤組織中的陽性表達(dá)率為50.0%(53/106)，差異有統(tǒng)計學(xué)意義(P＜0.01)。STAT3表達(dá)與膠質(zhì)瘤WHO分級相關(guān)，在Ⅰ、Ⅱ、Ⅲ、Ⅳ級中的陽性表達(dá)率分別為30.77%(4/13)，32.14%(9/28)，61.29%(19/31)，61.76%(21/34)，差異有統(tǒng)計學(xué)意義(P＜0.05)。STAT3表達(dá)與患者性別、年齡及腫瘤發(fā)生部位無相關(guān)性(P＞0.05)。 2.β-catenin在正常腦組織中未發(fā)現(xiàn)陽性表達(dá)，在膠質(zhì)瘤組織中的陽性表達(dá)率為86.79%(92/106)，差異有統(tǒng)計學(xué)意義(P＜0.01)。β-catenin表達(dá)與膠質(zhì)瘤WHO分級相關(guān)，在Ⅰ、Ⅱ、Ⅲ、Ⅳ級中的陽性表達(dá)率分別為61.54%(8/13)，82.14%(23/28)，90.32%(28/31)，97.06%(33/34)，差異有統(tǒng)計學(xué)意義(P＜0.01)。β-catenin表達(dá)與患者性別、年齡及腫瘤發(fā)生部位無相關(guān)性(P＞0.05)。 3.VEGF在正常腦組織中未發(fā)現(xiàn)陽性表達(dá)，在膠質(zhì)瘤組織中的陽性表達(dá)率為58.49%(62/106)，差異有統(tǒng)計學(xué)意義(P＜0.01)。VEGF表達(dá)與膠質(zhì)瘤WHO分級相關(guān)，在Ⅰ、Ⅱ、Ⅲ、Ⅳ級中的陽性表達(dá)率分別為15.38%(2/13)，39.29%(11/28)，58.06%(18/31)，91.18%(31/34)，差異有統(tǒng)計學(xué)意義(P＜0.01)。VEGF表達(dá)與患者性別、年齡及腫瘤發(fā)生部位無相關(guān)性(P＞0.05)。 4.在膠質(zhì)瘤組織中，STAT3和β-catenin同為陽性表達(dá)53例，陰性表達(dá)14例，，二者呈正相關(guān)（r=0.801，P＜0.01）。STAT3和VEGF同為陽性表達(dá)50例，陰性表達(dá)41例，二者呈正相關(guān)（r=0.844，P＜0.01）。β-catenin和VEGF同為陽性表達(dá)62例，陰性表達(dá)14例，二者呈正相關(guān)（r=0.864，P＜0.01）。 結(jié)論 1.STAT3在膠質(zhì)瘤組織中的陽性表達(dá)與正常腦組織中的表達(dá)差異有統(tǒng)計學(xué)意義。STAT3在膠質(zhì)瘤組織中的表達(dá)與WHO分級相關(guān)；而與研究對象的性別、年齡和腫瘤發(fā)生的部位三個臨床觀察指標(biāo)之間的差異無統(tǒng)計學(xué)意義。 2.β-catenin在膠質(zhì)瘤組織中的陽性表達(dá)與正常腦組織中的表達(dá)差異有統(tǒng)計學(xué)意義。β-catenin在膠質(zhì)瘤組織中的表達(dá)與WHO分級相關(guān)；而與研究對象的性別、年齡和腫瘤發(fā)生的部位三個臨床觀察指標(biāo)之間的差異無統(tǒng)計學(xué)意義。 3.VEGF在膠質(zhì)瘤組織中的陽性表達(dá)與正常腦組織中的表達(dá)差異有統(tǒng)計學(xué)意義。VEGF在膠質(zhì)瘤組織中的表達(dá)與WHO分級相關(guān)；而與研究對象的性別、年齡和腫瘤發(fā)生的部位三個臨床觀察指標(biāo)之間的差異無統(tǒng)計學(xué)意義。 4.在膠質(zhì)瘤組織中STAT3、β-catenin和VEGF的表達(dá)兩兩之間呈正相關(guān)。
[Abstract]:Objective to study the protein expression of STAT3, beta -catenin and VEGF in glioma and normal brain tissue, to explore the role of the three in the occurrence, development, proliferation and infiltration of human glioma, and to analyze the relationship between the three and the clinical observation of glioma (sex, age, tumor site and glioma WHO classification) and the three in glioma. The correlation expressed in tissues can guide the judgement of malignant degree and prognosis of gliomas, and provide a theoretical basis for finding new targets of glioma targeted therapy.
Methods 106 specimens of glioma deposited wax from January 2009 to December 2012, the first people's Hospital of Lianyungang, Xuzhou Medical College, were collected and collected in archived cases. Among them, 62 men and 44 women were male and female, the sex ratio of men and women was 1.4:1; the youngest was 11 years old, the largest was 78 years old, and the average age of 46.4 years of brain glioma patients was 46.4 years old. Pathological data: all glioma specimens were diagnosed by surgical excision in the Department of neurosurgery and confirmed by pathological diagnosis. According to the latest standard of WHO classification of central nervous system tumor WHO (2007), the diagnostics of the first people's Hospital of the first people's hospital were divided into 1, II, III, IV grade. 41 cases of low grade group (grade I, grade II), and 65 cases of advanced group (III, IV); Grade I was 13, class II was 28, grade III was 31, grade IV was 34. 10 normal brain tissue specimens from the control group were obtained through intracranial decompression. The immunohistochemical Envision method was used to detect the expression of STAT3, beta -catenin and VEGF in human glioma tissue, and the data were analyzed with clinicopathological data. The results should be obtained. The data were processed by SPSS19.0 statistical software. Rank sum test and Spearman correlation analysis were used according to data type. The difference between P < 0.05 was statistically significant.
Result
The positive expression of 1.STAT3 was not found in normal brain tissue. The positive expression rate in glioma tissues was 50% (53/106), and the difference was statistically significant (P < 0.01).STAT3 expression was related to the WHO classification of glioma. The positive expression rate in grade I, II, III, and IV was 30.77% (4/13), 32.14% (9/28), 61.29% (19/31), 61.76% (21/34), and the difference was unified. There was no correlation between the expression of.STAT3 (P < 0.05) and the sex, age and location of tumor (P > 0.05).
The positive expression rate of 2. beta -catenin in normal brain tissue was 86.79% (92/106), and the difference was statistically significant (P < 0.01). The expression of beta -catenin was associated with the WHO grading of glioma. The positive expression rates in grade I, II, III, and IV were 61.54% (8/13), 82.14% (23/28), 90.32% (28/31), 97.06% (33/34), respectively. The difference was statistically significant (P < 0.01). There was no correlation between the expression of beta -catenin and the sex, age and location of tumor (P > 0.05).
The positive expression of 3.VEGF was not found in normal brain tissue. The positive expression rate in glioma tissues was 58.49% (62/106), and the difference was statistically significant (P < 0.01).VEGF expression was related to the WHO classification of glioma. The positive expression rate in grade I, II, III, and IV was 15.38% (2/13), 39.29% (11/28), 58.06% (18/31), 91.18% (31/34), and the difference was unified. There was no correlation between the expression of.VEGF (P < 0.01) and the sex, age and location of tumor (P > 0.05).
4. in glioma tissue, STAT3 and beta -catenin were positive expression in 53 cases, negative expression in 14 cases, two positive correlation (r=0.801, P < 0.01).STAT3 and VEGF positive expression, 41 cases, two positive correlation (r=0.844, P < 0.01). 62 cases were positive expression of beta -catenin and VEGF, negative expression 14 cases, two positive correlation (r=0.864, P) < 0.01).
conclusion
The difference between the positive expression of 1.STAT3 in glioma tissues and the expression of normal brain tissue has statistical significance. The expression of.STAT3 in the glioma tissue is related to the WHO classification, but there is no significant difference between the three clinical observation indexes of the sex, age and the site of the tumor.
The positive expression of 2. beta -catenin in glioma tissues was significantly different from that in normal brain tissue. The expression of beta -catenin in glioma tissues was related to the WHO classification, but there was no significant difference between the sex, age and the site of the tumor. The difference was not statistically significant between the sex, age and the site of the tumor.
The difference between the positive expression of 3.VEGF in glioma tissues and the expression of normal brain tissue has statistical significance. The expression of.VEGF in the glioma tissue is related to the WHO classification, but there is no significant difference between the three clinical observation indexes of the sex, age and the site of the tumor.
4. there was a positive correlation between the expression of STAT3, beta -catenin and VEGF in glioma tissues 22.

【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.41

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 麥玉潔,李云濤,邱錄貴;β-catenin與惡性腫瘤[J];國外醫(yī)學(xué)(內(nèi)科學(xué)分冊);2005年05期

2 尤永平;劉寧;傅震;;中國膠質(zhì)瘤規(guī)范化治療現(xiàn)狀[J];中國腫瘤外科雜志;2012年03期

本文編號：1892536