本文選題：獼猴 + 動(dòng)物模型　； 參考：《安徽醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的應(yīng)用獼猴運(yùn)動(dòng)皮層部分切除術(shù),建立嬰幼獼猴痙攣型偏癱腦癱模型和評(píng)價(jià)方法。方法4只3月齡獼猴隨機(jī)分為正常對(duì)照組和手術(shù)模型組。手術(shù)模型組獼猴:運(yùn)用運(yùn)動(dòng)皮層部分切除術(shù),切除右側(cè)大腦外側(cè)裂以上至半球間裂的中央前回皮質(zhì)及額上回后部(正前中溝以前約0.3-0.5cm)皮質(zhì),深度約0.5-0.6cm。術(shù)后進(jìn)行連續(xù)攝像,觀察有無(wú)左側(cè)肢體運(yùn)動(dòng)功能障礙和動(dòng)靜態(tài)的姿勢(shì)異常;徒手檢查和參考應(yīng)用改良人Ashworth量表評(píng)定肌張力,應(yīng)用自編粗大運(yùn)動(dòng)及精細(xì)運(yùn)動(dòng)評(píng)估量表檢測(cè)兩組運(yùn)動(dòng)障礙的量化指標(biāo),運(yùn)用9.4T MRI檢測(cè)顱腦的影像學(xué)改變。結(jié)果(1)手術(shù)模型組于術(shù)后立刻出現(xiàn)左側(cè)肢體癱瘓,左上肢不能正常抬舉,左下肢癱瘓,左側(cè)肢體跛行明顯,進(jìn)食時(shí)主要以右側(cè)肢體為主;手術(shù)組術(shù)后一周時(shí)活動(dòng)量明顯低于正常組,評(píng)分也低于正常組;2到3周后活動(dòng)量增加,評(píng)分也開(kāi)始升高,直至8周后,評(píng)分基本穩(wěn)定。手術(shù)模型組和正常對(duì)照組的粗大運(yùn)動(dòng)評(píng)分在術(shù)后各個(gè)時(shí)期不完全相同(Friedman檢驗(yàn),χ2值為33.939,P0.05),精細(xì)運(yùn)動(dòng)評(píng)分在術(shù)后各個(gè)時(shí)期亦不完全相同(Friedman檢驗(yàn),χ2值為37.526,P0.05);(2)手術(shù)模型組術(shù)后姿勢(shì)為靜止時(shí)向左側(cè)傾斜,蹲坐于猴籠一角,左上肢搭于猴籠網(wǎng)上以維持姿勢(shì)平衡,運(yùn)動(dòng)時(shí)仍有向左側(cè)傾斜,模擬出了人典型的痙攣型偏癱腦癱的臨床表現(xiàn);(3)徒手檢查肌張力的改變:手術(shù)模型組術(shù)后即出現(xiàn)左側(cè)肢體癱瘓和肌張力降低,5周后左側(cè)肌張力逐漸開(kāi)始增高,并逐漸加重至3級(jí),持續(xù)到現(xiàn)在,肌張力在術(shù)后5周至術(shù)后14周術(shù)后各個(gè)時(shí)期不完全相同(Friedman檢驗(yàn),χ2值為20.713,P0.05);(4)術(shù)后3周頭顱MRI顯示右側(cè)運(yùn)動(dòng)皮層切除術(shù)后疤痕組織形成,支持偏癱腦癱模型的影像學(xué)改變。結(jié)論(1)應(yīng)用一側(cè)運(yùn)動(dòng)皮層部分切除方法可成功建立嬰幼獼猴痙攣型偏癱腦癱模型;(2)通過(guò)攝像觀察獼猴的運(yùn)動(dòng)功能障礙和姿勢(shì)異常表現(xiàn),以及徒手檢查肌力肌張力情況,結(jié)合自編適用于獼猴神經(jīng)行為學(xué)評(píng)分和頭顱影像學(xué)改變可準(zhǔn)確評(píng)價(jià)獼猴腦性癱瘓偏癱模型的建立;(3)真切地模擬出人類嬰幼兒腦外傷性痙攣型偏癱腦癱模型,為腦癱的病理機(jī)制和臨床康復(fù)治療的研究提供一個(gè)科學(xué)的平臺(tái)。
[Abstract]:Objective to establish the model of cerebral palsy with spastic hemiplegia in infant rhesus monkey by partial resection of motor cortex. Methods four 3-month-old macaques were randomly divided into normal control group and surgical model group. In the model group, the cortex of precentral gyrus and posterior part of superior frontal gyrus (0.3-0.5 cm before anterior middle sulcus) was removed from right lateral fissure to interhemispheric fissure by partial resection of motor cortex, and the depth was about 0.5-0.6 cm. Postoperative continuous imaging was performed to observe whether there were left limb motor dysfunction and dynamic and static postural abnormalities, and muscle tension was evaluated with the improved human Ashworth scale. The quantitative indexes of motor disorders in the two groups were measured by using the self-made gross motion assessment scale and fine motion assessment scale, and the imaging changes of brain were detected by 9.4 T MRI. Results 1) in the operation model group, the left limb paralysis appeared immediately after operation, the left upper limb could not be lifted normally, the left lower extremity was paralyzed, the left limb claudication was obvious, and the main food intake was the right limb. The activity volume of the operation group was significantly lower than that of the normal group one week after operation, and the score was also lower than that of the normal group after 2 to 3 weeks, and the activity quantity increased, and the score began to increase, until 8 weeks later, the score was basically stable. The gross motor scores of the operation model group and the normal control group were not exactly the same as those of the postoperative model group and the normal control group. The 蠂 2 value of the model group was 33.939% (P 0.05), and the fine motor score was not exactly the same as the Friedman test and the 蠂 2 value was 37.526% P 0.05% (P 0.05) after operation in the model group. Tilt to the left when standing still, Crouching in the corner of the monkey cage, with the left upper limb resting on the cage net to maintain balance in posture, still tilting to the left when moving. The clinical manifestation of typical hemiplegic cerebral palsy in human was simulated. The changes of muscle tension were examined with bare hand: the left limb paralysis and the reduction of muscle tension appeared in the operation model group after 5 weeks, and the tension of the left side began to increase gradually after 5 weeks. The muscle tension was not exactly the same at every postoperative period from 5 weeks to 14 weeks after operation. The 蠂 2 value was 20.713 (P 0.05) 3 weeks after operation. The MRI showed the scar tissue formation after right motor cortex resection 3 weeks after operation. To support the imaging changes of hemiplegic cerebral palsy model. Conclusion 1) the model of spastic hemiplegic cerebral palsy in infantile rhesus monkeys can be successfully established by partial resection of unilateral motor cortex. The model of cerebral palsy hemiplegia in rhesus monkey can be accurately evaluated by using self-made neurobehavioral score and cranial imaging changes. The model of traumatic cerebral spastic hemiplegia in human infants can be truly simulated. To provide a scientific platform for the study of pathological mechanism and clinical rehabilitation of cerebral palsy.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R742.3;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 尹燕;金科;;磁共振彌散張量在偏癱型腦癱患兒康復(fù)中的應(yīng)用[J];醫(yī)療裝備;2015年09期

2 劉博虎;尹芳;岑鍵昌;劉俊;蒲軍;;猴腦嚴(yán)重缺血后選擇性腦深低溫灌注對(duì)神經(jīng)功能的影響[J];昆明醫(yī)科大學(xué)學(xué)報(bào);2015年03期

3 張朋;劉鵬;劉杰;王穎;高修成;張見(jiàn);;磁共振擴(kuò)散張量成像在嬰幼兒腦癱診斷中的應(yīng)用價(jià)值[J];中國(guó)醫(yī)療設(shè)備;2015年02期

4 秦明;朱虹;魏云艷;王德廣;;腦癱模型的制作與鑒定[J];中國(guó)婦幼保健;2014年26期

5 Yiwen Wang;Qin Fang;Neng Gong;;Motor assessment of developing common marmosets[J];Neuroscience Bulletin;2014年03期

6 盧英;蔡光先;譚琥;鄧叔華;易健;劉柏炎;;大腦中動(dòng)脈線栓法腦缺血模型制作的改進(jìn)及經(jīng)驗(yàn)探討[J];湖南中醫(yī)藥大學(xué)學(xué)報(bào);2014年01期

7 魏鵬緒;;關(guān)于改良Ashworth量表的探討[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2014年01期

8 Yadong Yu;Liang Li;Xinzhong Shao;Fangtao Tian;Qinglu Sun;;Establishing a rat model of spastic cerebral palsy by targeted ethanol injection[J];Neural Regeneration Research;2013年34期

9 穆學(xué)濤;馬林;;磁共振對(duì)腦癱的診斷價(jià)值及應(yīng)用前景[J];武警醫(yī)學(xué);2013年03期

10 戴如飛;蔡軍;劉寧;朱風(fēng)儀;李曉明;;大鼠腦內(nèi)囊出血模型的構(gòu)建及其評(píng)價(jià)(英文)[J];中國(guó)臨床康復(fù);2006年42期

相關(guān)碩士學(xué)位論文 前1條

1 白玉萌;電毀損錐體束與內(nèi)囊出血法建立痙攣性腦性癱瘓大鼠模型的比較[D];大連醫(yī)科大學(xué);2014年

，

本文編號(hào)：1880921