本文選題：腦梗死 + 動脈粥樣硬化斑塊��； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：探討腦梗死患者血漿中基質(zhì)金屬蛋白酶-9(MMP-9)、轉(zhuǎn)化生長因子-β1(TGF-β1)、金屬蛋白酶組織抑制劑-1(TIMP-1)表達(dá)與頸動脈粥樣硬化斑塊穩(wěn)定性的相關(guān)性。 方法：篩選84例腦梗死患者為腦梗死組和21例健康人群為對照組,根據(jù)頸動脈彩超檢查結(jié)果腦梗死組分為動脈硬化組、穩(wěn)定斑塊組、不穩(wěn)定斑塊組三個亞組。所有研究對象均收集一般臨床資料及血糖、血脂等實(shí)驗(yàn)室資料。采取酶聯(lián)免疫吸附試驗(yàn)法(ELISA)測定血漿中MMP-9、TGF-β1、TIMP-1水平。采用方差分析比較不同組間MMP-9、 TGF-β1、TIMP-1、MMP-9/TIMP-1差異；采用有序logistic回歸分析了解MMP-9、TGF-β1、TIMP-1水平的變化與動脈粥樣硬化斑塊穩(wěn)定性的關(guān)系。 結(jié)果：1.一般臨床資料及實(shí)驗(yàn)室資料比較：對照組與腦梗死組患者在性別、吸煙史、飲酒史分布及年齡、HDL、TG、CHOL水平上差異不具有統(tǒng)計(jì)學(xué)意義(P0.05),在糖尿病史、高血壓病史分布及LDL、FBS、IMT水平上差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；2.硬化組與對照組相比血漿MMP-9、TIMP-1含量增加,MMP-9/TIMP-1比值增大,血漿TGF-β1含量降低,除MMP-9/TIMP-1比值增大差異有統(tǒng)計(jì)學(xué)意義(P0.05),其余各指標(biāo)血漿含量變化均無統(tǒng)計(jì)學(xué)差異(P0.05)；與對照組、硬化組相比,穩(wěn)定斑塊組、不穩(wěn)定斑塊組血漿MMP-9、TIMP-1含量增加,MMP-9/TIMP-1比值增大,血漿TGF-β1含量降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；穩(wěn)定斑塊組與不穩(wěn)定斑塊組相比血漿MMP-9、TIMP-1含量增加,MMP9/TIMP1(?)比值增大,血漿TGF-β1含量降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3.有序變量logistic回歸分析提示：MMP-9不能使頸動脈斑塊易損風(fēng)險(xiǎn)增加(OR=0.965,95%CI:-2.572-3.815,P0.05);TGF-β1能使頸動脈斑塊易損風(fēng)險(xiǎn)降低(OR=0.582,95%CI:0.251-0.862, P0.05); TIMP-1能使頸動脈斑塊易損風(fēng)險(xiǎn)增加(OR=2.364;95%CI:1.415-2.374, P0.01)。 結(jié)論：1. TGF-β1、MMP-9、TIMP-1的水平改變可能參與了頸動脈粥樣硬化斑塊的形成及穩(wěn)定性的維持。2.TGF-β1、MMP-9/TIMP-1表達(dá)失調(diào)可能是頸動脈粥樣硬化斑塊發(fā)生及進(jìn)展的重要機(jī)制之一。3.TGF-β1是頸動脈粥樣硬化斑塊穩(wěn)定性的保護(hù)因素。
[Abstract]:Objective: to investigate the relationship between the expression of matrix metalloproteinase-9 (MMP-9), transforming growth factor- 尾 1 (TGF- 尾 1) and tissue inhibitor of metalloproteinase (TIMP-1) in patients with cerebral infarction and the stability of carotid atherosclerotic plaque. Methods: 84 patients with cerebral infarction were selected as cerebral infarction group and 21 healthy people as control group. According to the results of carotid ultrasound, cerebral infarction group was divided into three subgroups: arteriosclerosis group, stable plaque group and unstable plaque group. All subjects collected general clinical data, blood sugar, blood lipid and other laboratory data. The level of MMP-9 TGF- 尾 1 and TIMP-1 in plasma was determined by enzyme linked immunosorbent assay (Elisa). The differences of MMP-9, TGF- 尾 1 and TIMP-1 / TIMP-1 were compared by ANOVA, and the relationship between the changes of MMP-9, TGF- 尾 1and TIMP-1 and the stability of atherosclerotic plaques was studied by logistic regression analysis. The result is 1: 1. Comparison of general clinical data and laboratory data: there were no significant differences in sex, smoking history, distribution of drinking history and age of HDLT TGG Chol between the control group and the cerebral infarction group (P 0.05), but there was no significant difference between the two groups in the history of diabetes mellitus. There were significant differences in the distribution of hypertension history and the IMT level of LDLN FBSU (P 0.05). Compared with the control group, the plasma MMP-9 / TIMP-1 ratio increased and the plasma TGF- 尾 1 content decreased in the sclerosing group, with the exception of the increase in the MMP-9/TIMP-1 ratio, there was no significant difference in the plasma levels of the other indexes (P 0.05), and there was no significant difference between the sclerosing group and the sclerosing group. In stable plaque group, the content of MMP-9 / TIMP-1 increased and the ratio of MMP-9 / TIMP-1 increased, the content of TGF- 尾 1 decreased, the difference was statistically significant (P 0.05), and the content of MMP-9 and TIMP-1 in stable plaque group was higher than that in unstable plaque group (P < 0.05). The plasma TGF- 尾 1 content decreased with the increase of the ratio, and the difference was statistically significant. The logistic regression analysis showed that MMP-9 could not increase the risk of carotid plaque vulnerability. CI: -2.572-3.815 and TGF- 尾 1 could reduce the risk of carotid plaque vulnerability by 0.58295 CI: 0.251-0.862, P 0.055.The TIMP-1 could increase the risk of carotid plaque vulnerability by 2.36495% CI 1.415-2.374, P0.01. Conclusion 1. TGF- 尾 1, MMP-9 and TIMP-1 may be involved in the formation and stability of carotid atherosclerotic plaques. 2. TGF- 尾 1 MMP-9 / TIMP-1 expression imbalance may be one of the important mechanisms of carotid atherosclerotic plaque occurrence and progression. 3. TGF- 尾 1 is carotid atherosclerotic plaque. The protective factor of stability.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.3

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相關(guān)期刊論文 前2條

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本文編號：1839846