本文選題：癲癇 + 膠質(zhì)細(xì)胞　； 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的化學(xué)刺激wistar大鼠,建立癲癇模型,觀察大鼠癲癇發(fā)作的特點(diǎn)及組織形態(tài)學(xué)改變；并在不同時(shí)間點(diǎn)觀察星形膠質(zhì)細(xì)胞膠質(zhì)原纖維酸性蛋白(GFAP)在海馬中的表達(dá)、分布的動(dòng)態(tài)變化過(guò)程,探討GFAP在腦室內(nèi)注入白細(xì)胞介素1β(interleukin-1β,IL-1β)致癲癇模型海馬區(qū)的表達(dá)及意義。 方法健康雄性Wistar大鼠60只,分為2組：實(shí)驗(yàn)組(側(cè)腦室注射IL-1β),對(duì)照組(側(cè)腦室注射等量的生理鹽水)。實(shí)驗(yàn)組分為發(fā)作組和非發(fā)作組,實(shí)驗(yàn)發(fā)作組分別在癲癇發(fā)作后7d、14d、21d.30d給予取腦。利用免疫熒光和Western Blot檢測(cè)海馬區(qū)GFAP的表達(dá)情況及隨時(shí)間變化的規(guī)律。 結(jié)果1.成功建立化學(xué)刺激大鼠癲癇模型。2.實(shí)驗(yàn)發(fā)作組GFAP的表達(dá)量高于對(duì)照組及實(shí)驗(yàn)未發(fā)作組；實(shí)驗(yàn)組內(nèi),致癇后30天表達(dá)達(dá)到高峰。 結(jié)論1.在IL-1β致癇模型中,IL-1β可能導(dǎo)致星形膠質(zhì)細(xì)胞增生；2.膠質(zhì)細(xì)胞增生可能是癲癇發(fā)作的病理學(xué)基礎(chǔ),導(dǎo)致了癲癇的發(fā)生發(fā)展。
[Abstract]:Objective to establish an epileptic model by chemical stimulation of wistar rats, observe the characteristics and histomorphologic changes of epileptic seizures in rats, and observe the expression of glial fibrillary acidic protein (GFAP) in hippocampus at different time points. To investigate the expression and significance of GFAP in hippocampal region of epileptic model induced by intraventricular injection of interleukin-1 尾 interleukin-1 尾 (IL-1 尾). Methods 60 healthy male Wistar rats were divided into two groups: experimental group (lateral ventricle injection of IL-1 尾) and control group (lateral ventricle injection of the same amount of normal saline). The experimental group was divided into attack group and non-seizure group. The expression of GFAP in hippocampus was detected by immunofluorescence and Western Blot. Result 1. Successful establishment of chemically stimulated rat epileptic model. 2. 2. The expression of GFAP in the experimental attack group was higher than that in the control group and the non-seizure group, and the expression reached the peak 30 days after epilepsy in the experimental group. Conclusion 1. In IL-1 尾 induced epilepsy model, IL-1 尾 may lead to astrocyte proliferation. Glial proliferation may be the pathological basis of epileptic seizures, leading to the occurrence and development of epilepsy.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R742.1

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