本文選題：癲癇 + 戊四氮��； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的: 采用戊四氮(PTZ)誘發(fā)大鼠慢性癲癇，動態(tài)觀察不同腦區(qū)高遷移率族蛋白-1(High-mobility group box-1,HMGB-1)含量的變化，以及探討HMGB-1與癲癇的關(guān)系。 方法: 1、慢性癲癇大鼠模型的建立 SD雄性大鼠32只，將其隨機分為對照組（A組，n=8）和實驗組（n=24），對照組予腹腔注射40mg·kg-1的生理鹽水，實驗組分別腹腔注射不同濃度的PTZ,其中B組（20mg·kg-1）、C組（40mg·kg-1）、D組（60mg·kg-1），每次注射后密切觀察大鼠行為變化至少1小時，記錄下大鼠點燃所需天數(shù)、潛伏期時間、點燃的數(shù)量、存活的數(shù)量。 2、PTZ致癲大鼠不同腦區(qū)HMGB-1的表達 SD雄性大鼠40只,將其隨機分為正常對照組(A組，n=8)和實驗組（n=32）。實驗組大鼠采用PTZ40mg·kg-1一次性腹腔注射，對照組大鼠給予等量的生理鹽水腹腔注射，根據(jù)癲癇發(fā)作后的時間，于其發(fā)作后1h（B組）、6h（C組）、24h (D組)、72h（E組）取腦，采用免疫組織化學(xué)方法，動態(tài)觀察海馬組織中HMGB-1的變化；RT-PCR方法動態(tài)觀察腦組織中額葉、海馬、中腦中HMGB-1的變化。 結(jié)果： 1、當PTZ濃度為20mg·kg-1時，發(fā)作程度低，不易點燃；當PTZ濃度為60mg·kg-1時，發(fā)作較為劇烈，易點燃，但發(fā)作進展快，死亡率比較高，大鼠存活率低；當PTZ濃度為40mg·kg-1時，發(fā)作程度逐漸加重，點燃率較高，大鼠存活率高，不易死亡，發(fā)作緩解后仍可自由活動； 2、熒光定量PCR顯示：幼鼠不同腦區(qū)（額葉、中腦、海馬）HMGB-1蛋白表達隨著時間的推移呈動態(tài)升高，其中額葉、海馬于6h開始升高（P＜0.05），24h達高峰（P＜0.05），與24h相比，，72h有所降低（P＜0.05），但仍高于對照組（P＜0.05），中腦1h、6h、24h與對照組比較差異無統(tǒng)計學(xué)意義（P0.05），72小時的HMGB-1蛋白表達明顯高于對照組，差異有顯著性（P0.05）； 3、海馬免疫組化結(jié)果顯示，各組HMGB-1蛋白平均光密度值與對照組相比逐步升高，其中，B組HMGB-1蛋白表達量與對照組相比有所升高，但差異無統(tǒng)計學(xué)意義（P0.05）；與對照組相比，C組、D組HMGB-1蛋白表達量逐漸增高，差異有統(tǒng)計學(xué)意義（P0.05）；E組蛋白表達量有減少趨勢，與D組相比，差異有統(tǒng)計學(xué)意義（P＜0.05），但亦顯著高于對照組，差異有統(tǒng)計學(xué)意義（P0.01）。 結(jié)論： 1、戊四氮腹腔注射法建立慢性癲癇動物模型的理想濃度為40mg·kg-1； 2、EP發(fā)作后HMGB-1蛋白表達明顯升高，HMGB-1與癲癇所致大腦損傷密切相關(guān)。
[Abstract]:Objective: Chronic epilepsy was induced by pentylenetetrazol (PTZ) in rats. The changes of high mobility group box-1 HMGB-1) in different brain regions were observed dynamically, and the relationship between HMGB-1 and epilepsy was investigated. Methods: 1. Establishment of chronic epilepsy rat model Thirty-two male SD rats were randomly divided into two groups: control group (n = 8) and experimental group (n = 24). The control group was intraperitoneally injected with normal saline of 40mg kg-1. The experimental group was intraperitoneally injected with different concentrations of PTZs, including group B (20 mg / kg) and group C (40 mg / kg ~ (-1)) and group D (60 mg / kg ~ (-1)). The behavioral changes of rats were closely observed for at least one hour after each injection, and the days required for kindling, incubation period, the number of kindling and the number of surviving rats were recorded. Expression of HMGB-1 in different brain regions of epileptic rats induced by PTZ Forty Sprague-Dawley male rats were randomly divided into normal control group (group A) and experimental group (n = 32). The rats in the experimental group were injected intraperitoneally with PTZ40mg kg-1 and the rats in the control group were given the same amount of normal saline intraperitoneally. According to the time after seizure, the brain was taken from the rats in the 1h(B group at 6 h after seizure and in the D group at 24 h after seizure, and the rats in the control group were given the same amount of normal saline intraperitoneal injection. The changes of HMGB-1 in hippocampus were observed dynamically. The changes of HMGB-1 in frontal lobe, hippocampus and midbrain were observed dynamically by RT-PCR. Results: 1. When the concentration of PTZ was 20mg kg-1, the attack degree was low and it was not easy to ignite. When PTZ concentration was 60mg kg-1, the attack was more intense and easy to ignite, but the attack progression was faster, the mortality was higher, and the survival rate of rats was low. When the PTZ concentration was 40mg kg-1, the attack degree gradually aggravated. The rate of kindling was high, the survival rate of rats was high, and it was not easy to die. 2, fluorescence quantitative PCR showed that the expression of HMGB-1 protein in different brain regions (frontal lobe, midbrain, hippocampus) of young rats increased with the passage of time. The hippocampus began to increase at 6 h, P < 0.05 and reached the peak at 24 h (P < 0.05). Compared with the control group, the expression of HMGB-1 protein at 72h was lower than that in the control group (P < 0.05), but still higher than that in the control group (P < 0.05). There was no significant difference in the expression of HMGB-1 protein between the control group and the control group at 1h ~ 6h, and the expression of HMGB-1 protein was significantly higher than that of the control group at 72h (P 0.05). 3. The immunohistochemical results of hippocampus showed that the average optical density of HMGB-1 protein in each group was higher than that in control group, and the expression of HMGB-1 protein in group B was higher than that in control group. Compared with the control group, the expression of HMGB-1 protein in the C group increased gradually, and the difference was significant (P < 0.05), but it was also significantly higher than that in the control group (P < 0.05), the difference was significant (P < 0.05), and the difference was significant (P < 0.05), but it was also significantly higher than that in the control group (P < 0.05), and the difference was significant (P < 0.05), and the difference was significant (P < 0.05). The difference was statistically significant (P 0.01). Conclusion: 1. The ideal concentration of pentylenetetrazol in the establishment of chronic epileptic animal model was 40mg kg-1; 2the expression of HMGB-1 protein increased significantly after EP attack. HMGB-1 was closely related to brain injury induced by epilepsy.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R742.1

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