本文選題：自噬 + 肢體缺血后處理　； 參考：《華夏醫(yī)學(xué)》2017年02期

【摘要】：目的:探討肢體缺血后處理在腦缺血再灌注中激活自噬最佳時(shí)間窗。方法:用線栓法制備大鼠局灶性腦缺血再灌注模型,于大鼠腦缺血2 h再灌注24 h后進(jìn)行神經(jīng)功能缺損評(píng)分、腦梗死體積測(cè)定及HE染色、免疫組化檢測(cè)自噬相關(guān)蛋白LC3-Ⅱ。結(jié)果:與假手術(shù)組(Sham)比較,缺血再灌注組(I/R)出現(xiàn)明顯神經(jīng)功能缺損癥狀及腦梗死改變,LC3-Ⅱ表達(dá)不明顯,與I/R組比較,肢體缺血后處理組(Lpost C)神經(jīng)功能缺損癥狀有所改善,腦梗死體積顯著縮小,LC3-Ⅱ表達(dá)明顯增加,且在Lpost C0min亞組增加最顯著(P0.05)。結(jié)論:肢體缺血后處理對(duì)腦缺血再灌注損傷有保護(hù)作用,并且再灌注即刻干預(yù)激活自噬最顯著。
[Abstract]:Aim: to investigate the optimal time window for activation of autophagy during cerebral ischemia reperfusion after limb ischemia. Methods: the rat model of focal cerebral ischemia-reperfusion was established by the method of thread embolization. The neurological deficit score, cerebral infarction volume and HE staining were measured after 2 h of cerebral ischemia and 24 h of reperfusion. The autophagy related protein LC3- 鈪，

本文編號(hào)：1776614