本文選題：膠質(zhì)瘤 + ADAMTS蛋白酶　； 參考：《安徽醫(yī)科大學(xué)學(xué)報(bào)》2017年05期

【摘要】：目的研究人腦膠質(zhì)瘤中含血小板結(jié)合蛋白基序的解聚素金屬4(ADAMTS4)蛋白酶和ADAMTS5蛋白酶的表達(dá)及其與膠質(zhì)瘤病理分級(jí)之間的關(guān)系。方法采用免疫組化Envision二步法檢測(cè)ADAMTS4及ADAMTS5蛋白酶在44例不同級(jí)別腦膠質(zhì)瘤、11例瘤周腦組織中的表達(dá),計(jì)算陽性細(xì)胞百分率,并通過Image Pro Plusversion 6.0(IPP)圖像分析軟件測(cè)量計(jì)算其平均光密度(MOD)值。結(jié)果 (1)ADAMTS4和ADAMTS5蛋白酶在瘤周腦組織中不表達(dá)或極低表達(dá),而在膠質(zhì)瘤組織中高表達(dá),為棕黃色,主要表達(dá)定位于細(xì)胞外基質(zhì)及細(xì)胞質(zhì)中,兩組差異有統(tǒng)計(jì)學(xué)意義(P0.05)。進(jìn)一步研究顯示,其蛋白表達(dá)量隨著腫瘤級(jí)別的升高而升高,WHO分級(jí)中低級(jí)別、高級(jí)別間差異有統(tǒng)計(jì)學(xué)意義(P0.05)。(2)ADAMTS4與ADAMTS5蛋白酶在膠質(zhì)瘤中表達(dá)呈正相關(guān)性(χ~2=5.614,r=0.357,P0.05)。結(jié)論(1)ADAMTS4、ADAMTS5蛋白表達(dá)量隨著膠質(zhì)瘤級(jí)別的升高而升高,提示了ADAMTS4及ADAMTS5蛋白酶在膠質(zhì)瘤的進(jìn)展、侵襲中發(fā)揮著重要作用。(2)ADAMTS4與ADAMTS5蛋白表達(dá)呈正相關(guān)性,揭示了在膠質(zhì)瘤的發(fā)生發(fā)展中這兩種蛋白酶可能互相協(xié)同,兩者的過表達(dá)共同促進(jìn)了膠質(zhì)瘤的侵襲。
[Abstract]:Objective to study the expression of depolymerin metallodetrin 4 (ADAMTS4) protease and ADAMTS5 protease in human glioma and their relationship with the pathological grade of human glioma.Methods Immunohistochemical Envision two-step method was used to detect the expression of ADAMTS4 and ADAMTS5 protease in the surrounding brain tissues of 44 patients with different grade gliomas and to calculate the percentage of positive cells.The average optical density (MOD) value was calculated by the Image Pro Plusversion 6.0 image analysis software.Results ADAMTS4 and ADAMTS5 protease were not expressed or very low in the surrounding brain tissue, but were highly expressed in glioma tissues, which were mainly located in extracellular matrix and cytoplasm. The difference between the two groups was statistically significant (P 0.05).Further studies showed that the protein expression level increased with the increase of tumor grade. There was a positive correlation between the high grade ADAMTS4 and the expression of ADAMTS5 protease in gliomas (蠂 ~ 2 ~ 2 / 5.614 ~ 0.357P _ 0.05), and there was a positive correlation between the expression of ADAMTS4 and ADAMTS5 protease in gliomas (蠂 ~ (2 / 2) = 5.614 ~ (0.357) P 0.05).Conclusion ADAMTS4ADAMTS5 protein expression increased with the increase of glioma grade, suggesting that ADAMTS4 and ADAMTS5 protease play an important role in the progression and invasion of glioma. There is a positive correlation between ADAMTS4 and ADAMTS5 protein expression.These two proteases may cooperate with each other in the development of glioma, and their overexpression promotes the invasion of gliomas.
【作者單位】： 桂林醫(yī)學(xué)院;廣西壯族自治區(qū)南溪山醫(yī)院神經(jīng)外科;廣西壯族自治區(qū)南溪山醫(yī)院病理科;
【基金】：廣西自然科學(xué)基金項(xiàng)目(編號(hào):2013GXNSFAA019268)
【分類號(hào)】：R739.41
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本文編號(hào)：1764926