本文選題：轉(zhuǎn)化生長因子β激活激酶 + 自噬�。� 參考：《臨床麻醉學(xué)雜志》2017年02期

【摘要】：目的評價轉(zhuǎn)化生長因子β激活激酶1(TGFβ-activated kinase-1,TAK1)介導(dǎo)的細胞自噬在小鼠腦缺血-再灌注(ischemia-reperfusion,IR)損傷中的作用。方法雄性昆明小鼠72只,8周齡,體重(25.0±3.5)g,采用隨機數(shù)字表法分為六組:空白對照組(C組)、假手術(shù)組(S組)、IR組、TAK1短發(fā)夾RNA(shRNA)慢病毒組(T組)、陰性對照慢病毒組(Y組)和生理鹽水組(NS組),每組12只。T組、Y組和NS組分別以1μl/min側(cè)腦室注射TAK1shRNA慢病毒、陰性對照慢病毒和等量生理鹽水10μl。2周后IR組、T組、Y組和NS組制備腦IR損傷模型,缺血2h后恢復(fù)灌注;S組分離頸總動脈但不夾閉,其余手術(shù)步驟同IR組。再灌注24h后進行神經(jīng)功能缺陷評分(neurological severity scores,NSS);NSS評分完成后處死小鼠,取腦組織測定腦梗死體積;Western blot法檢測小鼠腦組織TAK1、LC3Ⅱ/LC3Ⅰ、Beclin1和p62蛋白含量。結(jié)果 IR組、T組、Y組和NS組NSS評分和腦梗死體積百分比明顯高于C組(P0.05);T組NSS評分和腦梗死體積百分比明顯低于IR組(P0.05)。IR組、Y組和NS組腦組織TAK1蛋白含量明顯高于C組(P0.05);T組腦組織TAK1蛋白含量明顯低于IR組(P0.05)。IR組、T組、Y組和NS組LC3Ⅱ/LC3Ⅰ比值、Beclin1蛋白含量明顯高于C組(P0.05),p62蛋白含量明顯低于C組(P0.05);T組腦組織LC3Ⅱ/LC3Ⅰ比值、Beclin1蛋白含量明顯低于,p62蛋白含量明顯高于IR組(P0.05)。結(jié)論 TAK1介導(dǎo)的細胞自噬參與小鼠腦缺血-再灌注損傷機制。
[Abstract]:Objective to evaluate the role of transforming growth factor 尾 -activated kinase (1(TGF 尾 -activated kinase-1 tak 1) -mediated autophagy in cerebral ischemia-reperfusion reperfusion injury in mice.Methods 72 male Kunming mice were 8 weeks old.TAK1shRNA lentivirus was injected into the lateral ventricle of 1 渭 l/min in group A and group NS, respectively.After 10 渭 l.2 weeks of negative control lentivirus and the same amount of normal saline, brain IR injury model was established in group T and group NS. The common carotid artery was isolated but not clipped in group S after 2 h ischemia. The other surgical procedures were the same as those in group IR.After 24 hours of reperfusion, the mice were killed by neurological severity scoress-NSSSS-NSS score. The contents of TAK1LC3 鈪，

本文編號：1739048