本文選題：跑臺運(yùn)動(dòng)訓(xùn)練　切入點(diǎn)：腦缺血　出處：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：通過觀察跑臺訓(xùn)練對腦缺血大鼠腦缺血周邊區(qū)血管再生及MT1-MMP表達(dá)的情況，探討跑臺訓(xùn)練對大鼠腦缺血后微血管生成的影響及其可能機(jī)制。 方法：隨機(jī)將42只成年雄性SD大鼠分為：假手術(shù)組(n=6)，模型組(n=18)和運(yùn)動(dòng)組(n=18)；采用改良Zea-Longa線栓法制備大鼠大腦中動(dòng)脈栓塞（MiddleCerebral Artery Occlusion，MCAO）模型；在制備動(dòng)物模型前，，所有大鼠先適應(yīng)性跑臺訓(xùn)練3天；模型制備成功后24小時(shí)對運(yùn)動(dòng)組大鼠進(jìn)行跑臺運(yùn)動(dòng)訓(xùn)練，采用改良的神經(jīng)行為學(xué)評分方法（mNSS），分別在造模成功后4天、7天、14天對各模型組及運(yùn)動(dòng)組大鼠的神經(jīng)功能恢復(fù)情況進(jìn)行評價(jià)。分別于術(shù)后4天、7天、14天將大鼠斷頭取腦，用MVD法分析計(jì)數(shù)各組大鼠缺血腦組織周邊區(qū)微血管數(shù)量；采用Western印跡法、免疫熒光組織化學(xué)法檢測MT1-MMP的表達(dá)。 結(jié)果：與MCAO組大鼠相比，運(yùn)動(dòng)組大鼠腦缺血周邊區(qū)的微血管數(shù)量及MT1-MMP表達(dá)水平明顯增高，差異具有統(tǒng)計(jì)學(xué)意義（P0.05）。 結(jié)論： 跑臺運(yùn)動(dòng)訓(xùn)練明顯促進(jìn)大鼠腦缺血周邊區(qū)微血管新生，并能改善腦缺血后神經(jīng)功能；其機(jī)制可能與MT1-MMP上調(diào)表達(dá)有關(guān)。
[Abstract]:Aim: to observe the effect of treadmill training on vascular regeneration and MT1-MMP expression in ischemic area of rats with cerebral ischemia, and to explore the effect of treadmill training on microangiogenesis after cerebral ischemia in rats and its possible mechanism.Methods: Forty-two adult male Sprague-Dawley rats were randomly divided into sham operation group (n = 6) and exercise group (n = 18). The middle cerebral artery occlusion (MCAO) model was established by modified Zea-Longa thread embolization method.All the rats were trained with adaptive treadmill for 3 days, and the rats in the exercise group were trained at 24 hours after the establishment of the model.A modified neurobehavioral scoring method was used to evaluate the recovery of neural function in rats of each model group and exercise group at 4 days, 7 days and 14 days after the establishment of the model.After 4 days, 7 days and 14 days after operation, the rat head was cut off and the number of microvessels in the ischemic brain tissue was counted by MVD method, and the expression of MT1-MMP was detected by Western blotting and immunofluorescence histochemistry.Results: compared with the MCAO group, the number of microvessels and the expression of MT1-MMP in the peripheral areas of cerebral ischemia in the exercise group were significantly higher than those in the MCAO group, and the difference was statistically significant (P 0.05).Conclusion:The treadmill training can obviously promote the microangiogenesis and improve the neural function in the cerebral ischemic area of rats, and its mechanism may be related to the up-regulation of MT1-MMP expression.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.3

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