本文選題：原發(fā)性脊髓膠質(zhì)瘤　切入點(diǎn)：Tbx3　出處：《南華大學(xué)》2014年碩士論文

【摘要】：背景與目的： 原發(fā)性脊髓膠質(zhì)瘤約占椎管內(nèi)腫瘤20%，但在脊髓髓內(nèi)腫瘤中卻占到了80%以上，其主要病理分型包括：室管膜瘤、星形細(xì)胞瘤及少突膠質(zhì)細(xì)胞瘤，以室管膜瘤最為常見。目前脊髓膠質(zhì)瘤的治療以手術(shù)切除為主，并輔以放療及化療，但治療效果都不盡理想，易復(fù)發(fā)且常出現(xiàn)嚴(yán)重的神經(jīng)功能障礙的并發(fā)癥。隨著分子生物學(xué)的發(fā)展，分子靶向治療和基因治療為膠質(zhì)瘤的治療提供了新的治療方向，而目前關(guān)于脊髓膠質(zhì)瘤的研究尚不夠深入。 PTEN基因是繼p53基因后人類發(fā)現(xiàn)的又一重要的抑癌基因。其表達(dá)的蛋白作為一種腫瘤抑制因子，可以通過阻止過快的細(xì)胞生長、分裂速度或者抑制不受控制的細(xì)胞分裂方式，進(jìn)而調(diào)控細(xì)胞分裂周期。Tbx3是T-box基因家族成員之一，其表達(dá)的蛋白具有促進(jìn)胚胎干細(xì)胞分裂增殖的作用。近年來研究的熱點(diǎn)認(rèn)為其與多種惡性腫瘤尤其是乳腺癌的發(fā)生密切相關(guān)。有研究證實(shí)Tbx3的表達(dá)可以抑制PTEN的表達(dá)從而誘發(fā)腫瘤發(fā)生發(fā)展。 本研究的目的是采用免疫組化的方法回顧性分析Tbx3和PTEN在原發(fā)性脊髓膠質(zhì)瘤中的表達(dá)及其相關(guān)性，從而進(jìn)一步從分子生物學(xué)角度探討原發(fā)性脊髓膠質(zhì)瘤的發(fā)生機(jī)制，為該病的診治提供新的可行方案。 方法： 收集2010年-2013年確診為原發(fā)性脊髓膠質(zhì)瘤患者的腫瘤組織石蠟包塊共52例，其中室管膜瘤30例、星形細(xì)胞瘤12例、少突膠質(zhì)細(xì)胞瘤10例，以病理類型分為3組，，10例正常脊髓組織為對照組。采用免疫組化方法分析各組中Tbx3和PTEN的表達(dá)情況，免疫組化結(jié)果經(jīng)圖像采集后，采用平均光密度值代表陽性強(qiáng)度進(jìn)行定量分析。數(shù)據(jù)結(jié)果采用SPSS19.0統(tǒng)計(jì)軟件進(jìn)行方差分析各組差異，然后采用Pearson相關(guān)性分析法分析Tbx3和PTEN表達(dá)的相關(guān)性。 結(jié)果： Tbx3的各實(shí)驗(yàn)組平均光密度值均值均明顯高于正常組p0.05，而各實(shí)驗(yàn)組間，室管膜瘤組和少突膠質(zhì)細(xì)胞瘤組無明顯差異p＞0.05，星形細(xì)胞瘤組與室管膜瘤及少突膠質(zhì)細(xì)胞瘤組均有明顯差異p0.05，且其平均光密度值低于室管膜瘤及少突膠質(zhì)細(xì)胞瘤組。 PTEN的各實(shí)驗(yàn)組平均光密度值均值均明顯低于正常組，各實(shí)驗(yàn)組相對正常組均有明顯差異p0.05，且各組平均光密度值均低于正常對照組；而各實(shí)驗(yàn)組間，室管膜瘤組和少突膠質(zhì)細(xì)胞瘤組無明顯差異p＞0.05，星形細(xì)胞瘤組與室管膜瘤及少突膠質(zhì)細(xì)胞瘤組均有明顯差異p0.05，且其平均光密度值稍高于室管膜瘤及少突膠質(zhì)細(xì)胞瘤組。 在實(shí)驗(yàn)組及正常對照組中，PTEN表達(dá)與Tbx3表達(dá)之間均呈明顯負(fù)相關(guān)r=-0.971，p＜0.05，兩者之間負(fù)相關(guān)有統(tǒng)計(jì)學(xué)意義。 結(jié)論： 1.Tbx3在各組脊髓膠質(zhì)瘤病理類型中均有表達(dá)，在正常組中幾乎無表達(dá)。但在星形細(xì)胞瘤中較室管膜瘤及少突膠質(zhì)細(xì)胞瘤中表達(dá)較弱，為弱陽性。2.PTEN在各組脊髓膠質(zhì)瘤病理類型中較正常組均有表達(dá)下降。但在星形細(xì)胞瘤中較室管膜瘤及少突膠質(zhì)細(xì)胞瘤中表達(dá)稍強(qiáng)。3.Tbx3在原發(fā)性脊髓膠質(zhì)瘤中的表達(dá)上調(diào)，而PTEN的表達(dá)下調(diào)，兩者呈負(fù)相關(guān)關(guān)系。
[Abstract]:Background and Purpose :

The main pathological types of glioma in the spinal cord include : dymodymoma , astrocytic tumor and microglia cell tumor . The main pathological types include : dymodymoma , astrocytic tumor and microglia cell tumor . The main pathological types include : dymodymoma , star - like cell tumor and microglia cell tumor , but the treatment effect is not ideal , and the therapy of molecular targeted therapy and gene therapy provides a new treatment direction for glioma treatment , and the current research on glioma is not enough .

The expression of PTEN gene is one of the members of T - box gene family , and its expressed protein has the function of promoting the division and proliferation of embryonic stem cells . Recent studies have shown that the expression of Tbx3 is closely related to the occurrence of various malignant tumors , especially breast cancer .

The purpose of this study was to investigate the expression of Tbx3 and PTEN in primary glioma by immunohistochemistry .

Method :

The expression of Tbx3 and PTEN was analyzed by means of immunohistochemistry . The expression of Tbx3 and PTEN was analyzed by immunohistochemistry . The results of immunohistochemistry were used to analyze the expression of Tbx3 and PTEN in each group . All groups were analyzed by SPSS 10.0 statistical software . The correlation between Tbx3 and PTEN expression was analyzed by Pearson correlation analysis .

Results :

The mean optical density of Tbx3 group was significantly higher than that in normal group ( p > 0.05 ) , but there was no significant difference between experimental group , dymodymoma group and microglia tumor group ( p > 0.05 ) . The average optical density of Tbx3 group was significantly lower than that in normal group ( p > 0.05 ) .

The mean optical density of PTEN was significantly lower than that of the normal group , and the mean optical density of the experimental group was significantly lower than that of the normal group ( p < 0.05 ) , and the mean optical density of PTEN was lower than that of the normal group .
In the experimental group , there was no significant difference in p > 0.05 , there was a significant difference of p > 0.05 between the group of dymoma and microglia , and the mean value of optical density was slightly higher than that of dymodymoma and microglia cell tumor .

There was a significant negative correlation between the expression of PTEN and the expression of Tbx3 in the experimental group and the normal control group ( r = - 0.971 , p < 0.05 ) .

Conclusion :

1 . The expression of Tbx3 was expressed in the pathological types of glioma in each group , but the expression of PTEN was weak in the normal group .

【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.42

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