本文選題：蛛網(wǎng)膜下腔出血　切入點(diǎn)：LC-Ⅱ　出處：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年05期

【摘要】：目的適度自噬有利于提高神經(jīng)細(xì)胞的存活率;而自噬過度又會(huì)引起細(xì)胞的調(diào)亡。文中旨在探討SP600125對(duì)蛛網(wǎng)膜下腔出血(SAH)大鼠海馬區(qū)神經(jīng)細(xì)胞自噬及神經(jīng)細(xì)胞丟失的影響。方法將健康雄性清潔級(jí)SD大鼠40只按隨機(jī)化數(shù)字表法分為假手術(shù)組、模型組、二甲基亞砜(DMSO組)、SP600125組,每組10只。模型組、DMSO組和SP600125組應(yīng)用血管內(nèi)穿刺法制成大鼠SAH模型。采用3-0縫合線引入到右頸外動(dòng)脈,通過頸內(nèi)動(dòng)脈推進(jìn)刺破大腦前動(dòng)脈和大腦中動(dòng)脈分叉處,建SAH模型。假手術(shù)組除不刺破血管,其余操作均與模型組一致。SP600125組于造模前30 min,利用腦立體定位儀行側(cè)腦室注射3μg/μL SP600125溶液10μL。假手術(shù)組和模型組均注射等體積等滲鹽水,DMSO組注射等量的DMSO。24 h處死,HE染色觀察各組海馬區(qū)神經(jīng)元形態(tài)及數(shù)量;免疫組化染色及Western blot法對(duì)p-JNK蛋白以及自噬標(biāo)志物Beclin-1和LC3-Ⅱ進(jìn)行定性定量檢測(cè)。結(jié)果 HE結(jié)果顯示,與假手術(shù)組比較,模型組海馬區(qū)神經(jīng)元排列紊亂、細(xì)胞多呈多邊形、數(shù)量明顯減少;與模型組比較,SP600125組海馬區(qū)神經(jīng)元損傷程度有所減輕、細(xì)胞明顯增多。與假手術(shù)組大鼠海馬區(qū)Beclin-1、LC3-Ⅱ、p-JNK蛋白平均光密度值(1.56±0.28、1.60±0.30、1.58±0.32)比較,模型組(14.66±4.40、12.62±3.46、12.82±3.68)、DMSO組(13.85±3.85、11.59±4.52、13.03±3.53)和SP600125組(9.86±3.14、6.78±2.56、5.60±2.42)明顯升高(P0.05);SP600125組較模型組明顯降低(P0.05)。與假手術(shù)組大鼠海馬區(qū)Beclin-1、LC3-Ⅱ、p-JNK蛋白表達(dá)(0.136±0.014、0.126±0.012、0.102±0.009)比較,模型組(0.474±0.122、0.668±0.130、0.496±0.124)、DMSO組(0.432±0.102、0.628±0.113、0.416±0.094)和SP600125組(0.264±0.106、0.332±0.113、0.219±0.104)明顯升高(P0.05);而SP600125組較模型組明顯降低(P0.05)。結(jié)論SP600125對(duì)SAH后神經(jīng)元細(xì)胞具有保護(hù)作用,其機(jī)制可能與SP600125通過抑制JNK信號(hào)通路的活性使自噬適度激活有關(guān)。
[Abstract]:Objective moderate autophagy can improve the survival rate of nerve cells. The effect of SP600125 on neuronal autophagy and neuronal loss in hippocampal area of rats with subarachnoid hemorrhage was studied. The computerized digital table was divided into sham-operation group, Model group, dimethylsulfoxide DMSO group (DMSO group), SP600125 group (n = 10), model group (n = 10), DMSO group (n = 10) and SP600125 group (n = 10) were used to establish rat SAH model by intravascular puncture, and 3-0 suture line was applied to the right external carotid artery. The SAH model was established by propulsive puncture of the anterior cerebral artery and the middle cerebral artery by the internal carotid artery. The rest of the operations were consistent with those of the model group. SP600125 group was injected with 3 渭 g / 渭 L SP600125 solution 30 minutes before the model. The sham operation group and the model group were injected with the same amount of DMSO.24 h and HE staining. The morphology and number of hippocampal neurons in each group were observed. Immunohistochemical staining and Western blot method were used to detect p-JNK protein, autophagy markers Beclin-1 and LC3- 鈪，

本文編號(hào)：1667085