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  本文選題：神經(jīng)球蛋白　切入點(diǎn)：氯化血紅素　出處：《重慶醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的探討外源性神經(jīng)球蛋白與氯化血紅素對大鼠缺血腦組織的聯(lián)合保護(hù)作用及其可能機(jī)制。 方法將175只SD大鼠隨機(jī)分為5組（n=35），分別為假手術(shù)組，缺血組，氯化血紅素干預(yù)組，質(zhì)粒干預(yù)組，氯化血紅素及質(zhì)粒共同干預(yù)組。采用大鼠MCAO腦缺血模型，麻醉清醒后，行神經(jīng)行為學(xué)檢測，術(shù)畢24h檢測腦組織含水量、腦梗死體積比、原位細(xì)胞凋亡技術(shù)檢測神經(jīng)元凋亡、免疫熒光技術(shù)檢測Bcl-2蛋白表達(dá)及Western蛋白印跡技術(shù)分析Ngb蛋白、Bcl-2蛋白表達(dá)情況。 結(jié)果質(zhì)粒攜帶的外源性Ngb與Hemin聯(lián)合干預(yù)組在神經(jīng)行為學(xué)評分、腦組織含水量、腦梗死體積比方面顯著降低，凋亡神經(jīng)元數(shù)量減少，同時皮層神經(jīng)元Ngb及Bcl-2蛋白表達(dá)增加，均較單獨(dú)干預(yù)組有顯著差異（p0.05）。 結(jié)論在大鼠局灶性腦缺血條件下，外源性Ngb與Hemin聯(lián)合作用，可能通過增強(qiáng)Ngb蛋白的表達(dá)，實(shí)現(xiàn)Bcl-2蛋白表達(dá)增加，抑制皮層神經(jīng)元凋亡，顯著降低了凋亡神經(jīng)元數(shù)目、腦組織梗死體積比、腦組織含水量和神經(jīng)行為評分，，從而獲得對局灶性缺血后神經(jīng)元的更佳保護(hù)作用。
[Abstract]:Objective to investigate the combined protective effect of exogenous neuroglobulin and hemin on ischemic brain tissue in rats and its possible mechanism. Methods 175 Sprague-Dawley rats were randomly divided into 5 groups: sham operation group, ischemia group, hemin intervention group, plasmid intervention group, hemin and plasmid co-intervention group. The brain water content, the volume ratio of cerebral infarction and the apoptosis of neurons were detected by in situ apoptosis technique at 24 hours after operation. The expression of Bcl-2 protein was detected by immunofluorescence technique and the expression of Bcl-2 protein by Ngb protein was analyzed by Western Western blot. Results the neurobehavioral score, brain water content, cerebral infarction volume ratio, the number of apoptotic neurons and the expression of Ngb and Bcl-2 protein in cortical neurons were significantly decreased in the intervention group with exogenous Ngb and Hemin. There was significant difference between the two groups (P 0.05). Conclusion under the condition of focal cerebral ischemia, the combination of exogenous Ngb and Hemin may enhance the expression of Ngb protein, increase the expression of Bcl-2 protein, inhibit the apoptosis of cortical neurons, and decrease the number of apoptotic neurons. The cerebral infarction volume ratio, brain water content and neurobehavioral score were used to obtain a better protective effect on neurons after focal ischemia.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 魏偉;湯熠;魏麓云;;EAE大鼠腦紅蛋白表達(dá)變化研究[J];免疫學(xué)雜志;2010年12期

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