本文選題：膠質(zhì)瘤　切入點：超高效液相色譜-四極桿-靜電場軌道阱質(zhì)譜技術(shù)　出處：《分析測試學(xué)報》2017年12期 　論文類型：期刊論文

【摘要】：采用超高效液相色譜-四極桿-靜電場軌道阱質(zhì)譜(UHPLC-Q-OrbitrapHRMS)技術(shù)對膠質(zhì)瘤患者和正常對照人群的血漿進行代謝輪廓分析,篩選膠質(zhì)瘤代謝標志物,為其發(fā)病機制闡明和臨床早期診斷提供科學(xué)依據(jù)。通過對UHPLC-Q-OrbitrapHRMS采集得到的譜圖進行峰識別、峰匹配和去噪等處理后,應(yīng)用主成分和正交偏最小二乘-判別分析法對代謝組學(xué)數(shù)據(jù)進行統(tǒng)計分析,篩選VIP1.0及P0.05的差異代謝物,并進一步對其診斷能力進行評價。結(jié)果顯示,膠質(zhì)瘤患者的血漿代謝輪廓發(fā)生明顯變化,發(fā)現(xiàn)并鑒定得到10個差異代謝物,其中亮氨酸、纈氨酸、色氨酸、膽堿和�；撬嵩谀z質(zhì)瘤患者血漿中含量降低,組氨酸、檸檬酸、乳酸、肌酸和丙酮酸含量升高,與正常對照組比較具有顯著性差異(P0.05),提示氨基酸和能量等代謝異常可能對膠質(zhì)瘤的發(fā)生發(fā)展具有重要影響。此外,各差異性代謝物對膠質(zhì)瘤均顯示出較好的診斷能力(AUC0.8),可作為潛在診斷標志物。
[Abstract]:UHPLC-Q-OrbitrapHRMS (UHPLC-Q-OrbitrapHRMS) technique was used to analyze the metabolic profile of plasma in glioma patients and normal controls, and to screen the metabolic markers of gliomas by ultrahigh performance liquid chromatography-tetrapole-electrostatic field trap mass spectrometry (UHPLC-Q-OrbitrapHRMS). It provides a scientific basis for the elucidation of pathogenesis and early clinical diagnosis. After peak recognition, peak matching and denoising of the spectrum obtained by UHPLC-Q-OrbitrapHRMS, The principal component and orthogonal partial least squares discriminant analysis were used to analyze the metabolomics data, to screen the VIP1.0 and P05 metabolites, and to evaluate the diagnostic ability of the metabolites. Ten different metabolites were found and identified, among which leucine, valine, tryptophan, choline and taurine decreased in the plasma of glioma patients, histidine, citric acid, histidine, citric acid. The contents of lactic acid, creatine and pyruvate were significantly higher than those of the normal control group (P 0.05), suggesting that the metabolic abnormalities of amino acid and energy might play an important role in the occurrence and development of glioma. The different metabolites showed good diagnostic ability for gliomas and could be used as potential diagnostic markers.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院藥學(xué)部;鄭州大學(xué)第一附屬醫(yī)院神經(jīng)外科;鄭州大學(xué)第一附屬醫(yī)院檢驗科;
【基金】：北京醫(yī)衛(wèi)健康公益基金會醫(yī)學(xué)科學(xué)研究基金(YWJKJJHKYII-B17819)
【分類號】：O657.63;R739.41
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本文編號：1581305