本文關鍵詞： 腦梗死 腎素-血管緊張素-醛固酮系統(tǒng) 基因多態(tài)性 CISS分型　出處：《廣西醫(yī)科大學》2015年碩士論文　論文類型：學位論文

【摘要】：目的本研究擬探討腎素-血管緊張素-醛固酮系統(tǒng)(renin-angiotension-aldosterone system, RAAS)相關基因多態(tài)性與桂西壯族人群按中國缺血性卒中亞型分型(Chinese Ischemic Stroke Subclassification, CISS)的腦梗死亞型的相關性,從遺傳學角度研究腦血管病變的發(fā)病機制。方法采用聚合酶鏈反應(polymerase chain reaction, PCR)和基因測序技術對193例桂西壯族腦梗死患者和169例對照組的ACE、AGT、ATGR1及CYP11B2進行基因分型,根據(jù)CISS分型法將193例腦梗死患者分成大動脈粥樣硬化(large artery atherosclerosis, LAA)組、穿支動脈疾病(penetracing artery disease, PAD)組、心源性(cardiogenic stroke, CS)組、其他原因(oetiologies, OE)組和不明確病因(undetermined etiology,UE)組。通過多因素logistics回歸分析這四種基因與桂西壯族人群腦梗死各亞型的相關性。結果本對照研究共納入桂西壯族腦梗死患者193例以及169例健康體檢者對照。LAA組中ACE基因DD基因型(χ2=6.4036,P=0.047)和D等位基因(χ2=6.8803,P=-0.0087)頻率高于對照組,AGT基因TT基因型(χ2=13.5614,P=0.0011)和T等位基因(χ2=4.2823,P=0.0385)頻率明顯高于對照組。PAD組和CS組中的AGTR1基因AC基因型(分別為χ2=4.0641,P=0.0438；x2=4.9288,P=0.0260)和C等位基因(分別為x2=3.9129,P=-0.0479；x2=4.7864,P=0.0287)頻率高于對照組。五組腦梗死亞型中CYP11B2基因-344C/T TT基因型和T等位基因頻率與對照組無顯著性差異(P0.05)。多因素logistics回歸顯示LAA亞型中攜帶AGT基因TT基因型及攜帶ACE基因D等位基因可增加腦梗死風險分別為2.513倍(0R=2.513,95%CI：1.209～5.225)和1.702倍(OR=1.702,95%CI:1.012～2.863)。結論1.在桂西壯族人群中,RAAS相關位點基因多態(tài)性與腦梗死相關,RAAS相關基因中ACE基因D等位基因與AGT基因M235T TT基因型可能是大動脈粥樣硬化亞型腦梗死的遺傳易感基因。2.本研究未發(fā)現(xiàn)RAAS相關基因與腦梗死CS、CS、OE、UE亞型患者有相關性。
[Abstract]:Objective to investigate the relationship between gene polymorphism of renin-angiotension-aldosterone system (RAAS-Ras) and the subtype of Chinese Ischemic Stroke subtype (CISS) in Guanxi Zhuang population according to the subtype of ischemic stroke in China. Methods ACE-AGTATGR1 and CYP11B2 were genotyped by polymerase chain reaction (PCR) and gene sequencing in 193 patients with cerebral infarction in Guanxi Zhuang nationality and 169 controls. According to CISS classification, 193 patients with cerebral infarction were divided into three groups: large artery atherosclysis group, perforating artery disease artery disease group and cardiogenic cardiogenic stroke-group. The correlation between the four genes and the subtypes of cerebral infarction in the Zhuang nationality population in western Guangxi was analyzed by multivariate logistics regression analysis. Results this control study included 193 patients with cerebral infarction in western Guangxi. The frequencies of DD genotype and D allele of ACE gene in LAA group were significantly higher than those in control group (蠂 2 + 6.8803) and T allele (蠂 2 + 13.5614) and T allele (蠂 2 4.2823) respectively. The frequencies of DD genotype and D allele were significantly higher than those in control group and CS group. The frequencies of AC genotype (蠂 2 + 4.0641) and C allele (x 23.9129) were higher than those in the control group (蠂 ~ 2 + 4.0641). There was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group (P 0.05). The multivariate logistics regression analysis showed that there was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group (P < 0.05). The multivariate logistics regression analysis showed that there was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group. The results showed that carrying TT genotype of AGT gene and D allele of ACE gene in LAA subtype could increase the risk of cerebral infarction. The risk of cerebral infarction was increased by 2.513 times and 1.702 times than 1.209% and 1.702 times respectively. Conclusion\\\;\\\%\%??? The D allele of the ACE gene and the M235T TT genotype of the AGT gene may be the genetic susceptibility genes of the cerebral infarction subtype of atherosclerosis. This study has not found the correlation between the RAAS related gene and the patients with the subtype of cerebral infarction.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R743.3

【參考文獻】

相關期刊論文 前10條

1 王祺;章秋;;皖西地區(qū)漢族人群載脂蛋白A5啟動子區(qū)域基因多態(tài)性與血脂水平的相關性研究[J];安徽醫(yī)學;2010年05期

2 林慧中;林濤;陳慧;;CYP11B2基因多態(tài)性與缺血性腦卒中關聯(lián)性的Meta分析[J];吉林大學學報(醫(yī)學版);2013年03期

3 黃健;黃韻祝;王錦支;吳紅蘭;;載脂蛋白A5啟動子-1131T>C及56C>G基因多態(tài)性與血脂水平的相關性研究[J];貴州醫(yī)藥;2013年07期

4 朱旭明;謝國強;張健;孫偉峰;陳國千;;血管緊張素原基因M235T多態(tài)性與原發(fā)性高血壓相關性的Meta分析[J];重慶醫(yī)學;2013年31期

5 李宏芬;沈志霞;劉子洪;王穎杰;安潔;任燕;;血管緊張素原基因多態(tài)性及血糖水平與動脈硬化性腦梗死的相關性[J];國際檢驗醫(yī)學雜志;2014年24期

6 柳四新;譚紅;王家琪;;血管緊張素Ⅱ受體-1基因A1166C多態(tài)性與腦梗死的關系[J];臨床神經(jīng)病學雜志;2011年04期

7 ;Relationship between nerve injury-induced protein gene 2 polymorphism and stroke in Chinese Han population[J];Journal of Biomedical Research;2011年04期

8 李宏芬;王穎杰;沈志霞;;血管緊張素原基因M235T及α-內(nèi)收蛋白基因G460T多態(tài)性與腦梗死的關系[J];臨床神經(jīng)病學雜志;2012年06期

9 田國平;羅劍;程紅;陳元;孫莎艷;袁中華;曾俊中;周正偉;;原發(fā)性高血壓伴肥胖患者血漿RAAS激素水平和CYP11B2-344C/T基因多態(tài)性的相關性[J];中國動脈硬化雜志;2013年11期

10 岳蘊華;白旭東;張小寧;劉毓;毛潔萍;曾海波;地拉娜木;楊小英;米合熱依;;血清基質金屬蛋白酶9水平及其基因C1562T多態(tài)性與維族腦梗死患者頸動脈粥樣硬化斑塊穩(wěn)定性的關系[J];臨床神經(jīng)病學雜志;2014年02期

，

本文編號：1536544