本文關(guān)鍵詞： 動物模型 三叉神經(jīng)痛 滑石粉 大鼠　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:建立一種適合臨床研究的、簡單、易行、可靠的三叉神經(jīng)痛動物模型。方法:實驗一選取Wistar雄性大鼠20只,隨機(jī)分為兩組,一組在眶下神經(jīng)孔周圍注射30㳠滑石粉混懸液0.3ml,另一組注射同等劑量的生理鹽水,于術(shù)前3天以及術(shù)后3天、1周、2周、3周、4周、6周、8周、12周進(jìn)行機(jī)械痛閾行為學(xué)測定。觀察大鼠眶下神經(jīng)支配區(qū)域?qū)C(jī)械刺激的疼痛反應(yīng)閾值及疼痛行為學(xué)表現(xiàn)。實驗二選取Wistar雄性大鼠20只,隨機(jī)分為兩組,一組在眶下神經(jīng)孔周圍注射30㳠滑石粉混懸液0.3ml,另一組注射同等劑量的生理鹽水,于術(shù)前3天以及術(shù)后3天、1周、2周、4周進(jìn)行視頻行為學(xué)測定。攝像機(jī)放置在籠子前面1米處觀察大鼠探究行為、靜止行為、抓臉行為、搖頭行為、舔后肢行為和舔身體行為的次數(shù)和時間。實驗三選取Wistar大鼠20只,分為滑石粉注射組和對照組,每組各10只。按照實驗1的方法制作滑石粉三叉神經(jīng)痛模型。分別于術(shù)后3天、4周、8周、12周取大鼠眶下神經(jīng)、延髓組織作組織病理學(xué)觀察及脫髓鞘染色,應(yīng)用免疫組化的方法檢測眶下區(qū)組織腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-1β(IL-1β)以及延髓中疼痛物質(zhì)(p物質(zhì)、內(nèi)啡肽等)的表達(dá)。結(jié)果:實驗一實驗組大鼠于術(shù)后3天出現(xiàn)眶下神經(jīng)支配區(qū)域機(jī)械痛反應(yīng)閾值與術(shù)前及對照組相比明顯降低(P㩳0.01),大鼠易激惹,具有搔抓面部或攻擊行為。機(jī)械痛閾值降低持續(xù)至術(shù)后12周。實驗二視頻顯示:實驗組大鼠于術(shù)后3天出現(xiàn)探究行為、靜止行為、抓臉行為、搖頭行為的變化,與術(shù)前及對照組相比上述行為變化有統(tǒng)計學(xué)意義(P㩳0.01),舔后肢、舔身體行為無明顯變化。大鼠術(shù)后1周、2周、4周的探究行為、靜止行為、抓臉行為、搖頭行為變化進(jìn)一步明顯,與術(shù)前及對照組相比上述行為變化均有統(tǒng)計學(xué)意義(P㩳0.01)。舔后肢、舔身體行為與術(shù)前及對照組相比上述行為變化無統(tǒng)計學(xué)意義。實驗三實驗組術(shù)后3天組織病理學(xué)觀察:主要是炎癥表現(xiàn),炎性因子表達(dá)少.術(shù)后1周炎癥更劇烈免疫組化出現(xiàn)炎性因子表達(dá).術(shù)后4周炎性因子表達(dá)最高,4-12周炎性反應(yīng)逐漸減輕,局部瘢痕加重,可見壓迫眶下神經(jīng),脫髓鞘染色可見脫髓鞘表現(xiàn);延髓組織中疼痛物質(zhì)的表達(dá)于3天-3周無明顯變化,術(shù)后4-12周出現(xiàn)疼痛物質(zhì)表達(dá)增加并出現(xiàn)脫髓鞘表現(xiàn)。結(jié)論:1、眶下神經(jīng)干注射滑石粉可致眶下神經(jīng)支配區(qū)域出現(xiàn)痛覺超敏現(xiàn)象,自發(fā)性疼痛以及相關(guān)行為學(xué)改變;2、組織病理學(xué)及免疫組化檢查顯示該動物模型早期為炎性因子引起疼痛,晚期為眶下神經(jīng)壓迫致病。3、該動物模型可早期誘導(dǎo)疼痛且持續(xù)時間長,簡單易行,為目前較理想的三叉神經(jīng)痛動物模型。
[Abstract]:Objective: to establish a simple, easy and reliable animal model of trigeminal neuralgia suitable for clinical study. Methods: in experiment 1, 20 male Wistar rats were randomly divided into two groups. The talc powder suspension was 0.3 ml, and the other group was injected with the same dose of normal saline. The mechanical pain threshold was measured 3 days before operation and 3 weeks after surgery. The threshold of pain response to mechanical stimulation in the innervation region of the suborbital nerve and the behavior of pain behavior in the suborbital nerve innervation region of rats were observed. The mechanical pain threshold was measured at the end of 4 weeks, 6 weeks and 8 weeks and 12 weeks after operation. Experiment two selected 20 male Wistar rats, They were randomly divided into two groups. One group was injected around the suborbital foramen with 30? The talc powder suspension was 0.3 ml. Another group was injected with the same dose of normal saline. The video behavior was measured 3 days before operation and 3 days after operation. The camera was placed in front of the cage to observe the exploring behavior and static behavior of the rats. The number and time of face scratching, shaking head, licking hind limbs and licking body behavior. Experiment 3 selected 20 Wistar rats and divided them into talcum powder injection group and control group. The model of trigeminal neuralgia with talc powder was made according to the method of experiment 1 in each group. The suborbital nerve was taken from rats at 3 days, 4 weeks, 8 weeks and 12 weeks after operation, and the medulla oblongata was observed by histopathological observation and demyelinating staining. Tumor necrosis factor- 偽 (TNF- 偽), interleukin-1 尾 (IL-1 尾) and pain substance in medulla oblongata were detected by immunohistochemical method. Results: the mechanical pain response threshold of the suborbital innervation region in experimental group 1 was significantly lower than that in preoperation and control group. The mechanical pain threshold was decreased until 12 weeks after operation. Experiment 2 video showed that the rats in the experimental group showed the changes of inquiry behavior, static behavior, face grabbing behavior and shaking head behavior 3 days after operation. Compared with the preoperative and control groups, the changes of the above behaviors were statistically significant (P < 0.05). There was no significant change in the behavior of lapping hind limbs and licking the body. The behavior of inquiry, static behavior, face grabbing and shaking head were significantly changed in rats 1 week and 2 weeks after operation, compared with those before operation and control group. There was no significant difference in the behavior between the experimental group and the control group. The histopathological changes were observed 3 days after operation in the experimental group: inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation, inflammation. The expression of inflammatory factors was less. 1 week after operation, inflammatory factors were expressed more intensely by immunohistochemistry. The highest expression of inflammatory factors was observed at 4 to 12 weeks after operation. The inflammatory reaction gradually decreased and the local scar became more severe, and the suborbital nerve was compressed. Demyelination staining showed demyelination, and the expression of pain substance in medulla oblongata did not change significantly at 3 weeks after 3 days. At 4-12 weeks after operation, the expression of pain substance increased and demyelination appeared. Conclusion injection of talcum powder into the suborbital nerve trunk can induce hyperalgesia in the innervation region of the suborbital nerve. Spontaneous pain and related behavioral changes. Histopathological and immunohistochemical examination showed that the animal model was caused by inflammatory factors at the early stage and by suborbital nerve compression at the late stage. The animal model could induce pain early and last for a long time. It is an ideal animal model of trigeminal neuralgia.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R745.11;R-332

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