本文關(guān)鍵詞： Alu 膠質(zhì)瘤 甲基化　出處：《南通大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的通過檢測膠質(zhì)瘤患者血清及腫瘤組織中Alu甲基化水平的變化,結(jié)合臨床病例資料,分析并探討血清中Alu甲基化水平檢測對膠質(zhì)瘤早期診斷及預(yù)后判斷的意義。方法收集65例膠質(zhì)瘤患者腫瘤組織及對應(yīng)的血清標(biāo)本,以及30例健康人血清標(biāo)本作為對照,采用亞硫酸氫鹽測序(BSP)法(包括DNA提取與修飾、PCR擴增、測序)來檢測膠質(zhì)瘤患者血清、組織和正常人血清中Alu甲基化水平,結(jié)合臨床隨訪資料,進行統(tǒng)計分析。結(jié)果膠質(zhì)瘤患者血清中Alu平均甲基化水平為47.30%(35.40-54.25%),而正常人血清中是57.90%(55.25-61.45%),具有統(tǒng)計學(xué)差異(P0.01);腫瘤組織中Alu平均甲基化水平為40.30%(36.80-54.20%),與對應(yīng)的血清中的Alu甲基化水平具有較高的一致性(r=0.882);在腫瘤組織和病人血清組中,高級別組(14 WHOⅢ級,19 WHOⅣ級)的甲基化水平都低于低級別組(15 WHOⅠ級,17WHOⅡ級,P0.01,P0.01),另外Alu甲基化水平越高也預(yù)示著更高的生存率(P0.01,P0.01);ROC曲線分析曲線下面積(AUC)為0.861(0.789-0.933,P0.001),說明血清中Alu甲基化水平的檢測具有較高的診斷價值。結(jié)論血清游離DNA中Alu低甲基化的檢測對于膠質(zhì)瘤的早期診斷及預(yù)后判斷有著很好的臨床應(yīng)用價值。本實驗為血清游離DNA檢測在臨床上的廣泛應(yīng)用奠定了基礎(chǔ)。
[Abstract]:Objective to detect the level of Alu methylation in serum and tumor tissue of glioma patients and to combine the clinical data. The significance of serum Alu methylation level in early diagnosis and prognosis of glioma was analyzed and discussed. Methods tumor tissues and corresponding serum samples were collected from 65 patients with glioma. The serum samples of 30 healthy people were used as control, and the serum samples of glioma patients were detected by bisulfite sequencing method (including DNA extraction and modified PCR amplification, sequencing). The levels of Alu methylation in tissue and normal serum were combined with clinical follow-up data. Results the average methylation level of Alu in the serum of glioma patients was 47.300.35.40-54.25). In normal people, 57.90 was 55.25-61.45 and there was statistical difference (P 0.01). The average methylation level of Alu in tumor tissue was 40.30 and 36.80-54.20). The level of Alu methylation was consistent with the corresponding serum methylation level. In tumor tissue and patient serum group, the methylation level in high grade group (14 WHO 鈪，

本文編號：1461813