本文關(guān)鍵詞： 卒中后抑郁 小腦頂核 凋亡 形態(tài)學(xué) 大鼠　出處：《遼寧醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 觀察卒中后抑郁(post-stroke depression，PSD)模型大鼠小腦頂核細(xì)胞的形態(tài)學(xué)改變，并檢測頂核細(xì)胞的凋亡情況。通過觀察凋亡相關(guān)蛋白caspase-3、caspase-8、caspase-9及AIF（apoptosis-inducing factor，AIF）在該區(qū)域的表達(dá)，初步探討PSD大鼠小腦頂核細(xì)胞發(fā)生損傷的方式，探討小腦頂核損傷與PSD的關(guān)系。 方法 選用健康SD（Sprague-Dawley, SD）大鼠，隨機(jī)分為4組。對照組：給予假手術(shù)處理。卒中組：行大腦中動脈閉塞術(shù)（middle cerebral artery occlusion，MCAO）。抑郁組：慢性不可預(yù)見性溫和刺激（chronic unpredictable mildstimulation, CUMS）結(jié)合孤養(yǎng)。PSD組：MCAO后給予CUMS結(jié)合孤養(yǎng)。進(jìn)行行為學(xué)觀察和測試后，取腦組織，行尼氏染色，觀察各組大鼠頂核區(qū)域形態(tài)學(xué)改變；應(yīng)用末端脫氧核苷�；D(zhuǎn)移酶介導(dǎo)性dUTP切口末端標(biāo)記(Terminaldeoxynucleotidyl transferase-mediated dUTP nick end labeling, TUNEL)檢測神經(jīng)細(xì)胞凋亡情況，應(yīng)用免疫組化技術(shù)測定凋亡相關(guān)蛋白caspase-3、caspase-8、caspase-9及AIF的表達(dá)。 結(jié)果 1、大鼠行為學(xué)觀察和測試：與對照組及卒中組相比，PSD組大鼠體重增長速度減慢，糖水消耗量下降，水平運動及垂直運動降低，強(qiáng)迫游泳不動時間延長（P0.05，或P0.01）。 2、腦組織病理學(xué)觀察：尼氏染色結(jié)果，對照組尼氏小體正常存在。與對照組相比，PSD組小腦頂核細(xì)胞尼氏小體減少甚至消失。尼氏體在其余各組中均有減少，但程度不同。 3、TUNEL檢測：對照組小腦頂核可見極少量的凋亡細(xì)胞，考慮與生理性死亡有關(guān)。卒中組，，抑郁組和PSD組均有較多凋亡細(xì)胞出現(xiàn)，與對照組相比具有統(tǒng)計學(xué)意義（P0.05，或P0.01）。 4、免疫組化檢測結(jié)果：對照組caspase-3、caspase-8、caspase-9及AIF的表達(dá)強(qiáng)度均最低。與對照組比較，PSD組的caspase-3、caspase-8、caspase-9及AIF的表達(dá)均增加（P0.01）。 結(jié)論 1、PSD大鼠小腦頂核細(xì)胞存在損傷，該損傷可能與PSD發(fā)病有關(guān)。 2、PSD大鼠中小腦頂核的損傷與凋亡相關(guān)，可能通過caspase及非caspase介導(dǎo)的通路傳導(dǎo)，但具體的傳導(dǎo)通路未確定。
[Abstract]:Purpose Objective: to observe the morphological changes of cerebellar parietal nucleus cells in post-stroke PSD rats with post-stroke depression. The apoptosis of apical nucleus cells was detected. The apoptosis-related protein caspase-3 and caspase-8 were observed. The expression of caspase-9 and AIF(apoptosis-inducing factor AIFs in this region. To explore the damage mode of cerebellar parietal nucleus cells in PSD rats and the relationship between the injury of cerebellar parietal nucleus and PSD. Method Healthy SD rats were selected. They were randomly divided into 4 groups: control group: sham operation and stroke group: middle cerebral artery occlusion was performed during middle cerebral artery occlusion (MCAO). Depression group: chronic unpredictable mild stimulation of chronic unpredictable mildstimulation. CUMS combined with solitary. PSD group was given CUMS combined with solitary care. After behavioral observation and test, brain tissue was taken and stained by Nissl's staining. The morphologic changes of the parietal nucleus in each group were observed. Terminal deoxynucleotidyl transferase mediated dUTP incision end labeling (. Terminaldeoxynucleotidyl transferase-mediated dUTP nick end labeling. Tunel was used to detect neuronal apoptosis and the apoptosis-related protein caspase-3 and caspase-8 were detected by immunohistochemistry. Expression of caspase-9 and AIF. Results 1. Behavioral observation and test: compared with the control group and the stroke group, the rats in the PSD group had slower weight gain, lower consumption of sugar water, and lower horizontal and vertical movement. Forced swimming immobility prolonged P0.05, or P0.01. 2. Observation of brain histopathology: the results of Nissl staining showed that the Nissl corpuscles were normal in the control group, and compared with the control group. In PSD group, the Nissl corpuscles of cerebellar parietal nucleus cells decreased or even disappeared, and the Nissl bodies decreased in other groups, but to different degrees. 3Tunel detection: a very small number of apoptotic cells were found in the cerebellar parietal nucleus in the control group, which was related to physiological death. There were more apoptotic cells in the apoplexy group, depression group and PSD group. Compared with the control group, there was significant difference in P0.05, or P0.01. 4. The expression of caspase-3, caspase-8, caspase-9 and AIF were the lowest in the control group. The expression of caspase-3, caspase-8, caspase-9 and AIF increased in PSD group (P 0.01). Conclusion 1 the damage of cerebellar parietal nucleus cells in PSD rats may be related to the pathogenesis of PSD. 2 the damage of cerebellar parietal nucleus is related to apoptosis, which may be mediated by caspase and non-#en1# pathway, but the specific pathway is not determined.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.3

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