【摘要】：羥基磷灰石(簡(jiǎn)稱(chēng)HAP或HA),其組成和結(jié)構(gòu)與頸椎動(dòng)物的骨骼和牙齒基本一致。同時(shí),HA具有良好的生物相容性、生物活性、骨傳導(dǎo)性、骨增殖性、無(wú)毒性,常被用于骨組織的替代材料和生物體內(nèi)載體材料。在其他領(lǐng)域中,由于HA具有良好的離子交換性和吸附性,常用于處理生活用水中含量過(guò)高的氟離子,或者處理一些廢水中的重金屬離子。HA陶瓷的耐熱、耐濕范圍比較寬,抗衰老性能比較好,可作為濕敏半導(dǎo)體器件。這些性能是由HA的形貌、結(jié)構(gòu)和組成決定,因此,科學(xué)工作者們?cè)噲D制備出特定形貌的HA。本文中應(yīng)用了自組裝技術(shù)引入功能基團(tuán),例如引入氨基、羥基、羧基自組裝分子膜(SAMs)。SAMs接枝至鈦基底上,獲得平滑的功能界面。在功能界面上成功地水熱合成出均勻且無(wú)裂縫的HA涂層。通過(guò)AFM和XPS對(duì)界面進(jìn)行分析;用XRD、SEM、TEM對(duì)HA進(jìn)行物相和形貌等進(jìn)行分析。結(jié)果表明,此方法成功地在接枝了氨基、羥基、羧基自組裝分子膜的鈦基底上制備出高晶度、緊密的、定向的HA涂層。接枝了羧基的自組裝分子膜的鈦基底比氨基、羥基的更用利于羥基磷灰石的成核。探討了不同的反應(yīng)時(shí)間、pH、反應(yīng)溫度對(duì)HA涂層的成核和生長(zhǎng)的調(diào)控。這種方法成功的合成出高晶度、緊密的、附著的HA涂層。此外,體外細(xì)胞評(píng)價(jià)實(shí)驗(yàn)表明,和未處理鈦基底對(duì)比,HA涂層增強(qiáng)細(xì)胞的黏附性。研究了在高的pH值情況下,乙二胺四乙酸二鈉(Na_2EDTA)對(duì)HA晶體生長(zhǎng)的影響,反應(yīng)原料為無(wú)水氯化鈣,磷酸氫二鈉,按照不同濃度加入乙二胺四乙酸二鈉,調(diào)節(jié)溶液的pH,探討HA形貌和結(jié)構(gòu)變化。研究表明,高的pH能夠促進(jìn)HA微球形成,降低pH值,微球的緊密度逐漸下降;當(dāng)Na_2EDTA/Ca為2:2時(shí)候,有利于緊密的微球結(jié)構(gòu)形成;同時(shí)反應(yīng)溫度也影響著微球的緊密程度。探討了在高的pH,Na_2EDTA存在下,HA微球的形成機(jī)理。采用一種新的方法,氨水?dāng)U散法,合成多孔球形HA。用XRD、SEM、TEM、FT-IR和BET對(duì)HA進(jìn)行形貌、結(jié)構(gòu)和物相等分析。結(jié)果表明,氨水?dāng)U散調(diào)節(jié)反應(yīng)液的pH,促進(jìn)HA晶體的形成;反應(yīng)時(shí)間以及溫度對(duì)HA晶體的形貌起到很好的控制。在60℃氨水?dāng)U散24 h條件下,成功地制備出多孔的大比表面積HA微球。利用不同反應(yīng)時(shí)間,提出了多孔HA微球的形成機(jī)理。同時(shí),探討了多孔HA微球作為吸附劑,對(duì)重金屬鉛離子和銅離子的吸附性能,對(duì)Pb~(2+)和Cu~(2+)的吸附量分別為213.58±2.63和59.68±3.44 mg/g。根據(jù)上面的氨水?dāng)U散法,構(gòu)思了一種新的思路來(lái)合成多孔中空的HA微球。研究表明Na_2EDTA能夠控制Ca~(2+)離子在合成溶液中釋放和分布,促進(jìn)中空結(jié)構(gòu)的形成;同時(shí)反應(yīng)時(shí)間也在一定程度上影響著中空結(jié)構(gòu)形成。根據(jù)不同時(shí)間,對(duì)多孔中空HA微球形成機(jī)理進(jìn)行分析。
[Abstract]:The composition and structure of hydroxyapatite (HAP or HA),) are basically consistent with the bones and teeth of cervical vertebrae. At the same time, HA has good biocompatibility, bioactivity, bone conductivity, bone proliferation, non-toxicity, often used in bone tissue substitute materials and biological carrier materials. In other fields, because of its good ion exchange and adsorption properties, HA is often used to treat excessive fluoride ions in domestic water, or to treat heavy metal ions in some wastewater. Ha ceramics have a wide range of heat resistance and humidity resistance. Anti-aging performance is better, can be used as humidity-sensitive semiconductor devices. These properties are determined by the morphology, structure and composition of HA. Therefore, scientists try to produce HA. with specific morphology. In this paper, the self-assembly technique was used to introduce functional groups, such as amino, hydroxyl and carboxyl self-assembled molecular membrane (SAMs). SAMs was grafted onto titanium substrate to obtain a smooth functional interface. The uniform and crack-free HA coating was successfully synthesized on the functional interface by hydrothermal synthesis. The interface was analyzed by AFM and XPS, and the phase and morphology of HA were analyzed by XRD,SEM,TEM. The results showed that the HA coatings with high crystallinity, compact orientation and orientation were successfully prepared on titanium substrates grafted with amino, hydroxyl and carboxyl self-assembled molecular films. The titanium substrate of the self-assembled molecular membrane grafted with carboxyl group is more favorable to the nucleation of hydroxyapatite than the amino group and the hydroxyl group is more favorable to the nucleation of hydroxyapatite. The effects of different reaction time and pH, reaction temperature on the nucleation and growth of HA coating were discussed. This method successfully synthesized high crystallinity, compact, attached HA coating. In addition, cell evaluation in vitro showed that the HA coating enhanced the adhesion of the cells compared with the untreated titanium substrate. The effect of disodium ethylenediaminetetraacetate (Na_2EDTA) on the growth of HA crystal was studied at high pH value. The raw materials were anhydrous calcium chloride and disodium hydrogen phosphate, adding disodium ethylenediamine tetraacetate according to different concentration to adjust the pH, of the solution. The morphology and structure of HA were investigated. The results show that high pH can promote the formation of HA microspheres, decrease the pH value and decrease the density of the microspheres, and when the Na_2EDTA/Ca is 2: 2, it is beneficial to the formation of tight microspheres structure. At the same time, the reaction temperature also affects the degree of compactness of the microspheres. The formation mechanism of HA microspheres in the presence of high pH,Na_2EDTA was discussed. Porous spherical HA. was synthesized by ammonia diffusion method with a new method. The morphology, structure and content of HA were analyzed by XRD,SEM,TEM,FT-IR and BET. The results show that the pH, of the reaction solution can promote the formation of HA crystal, and the reaction time and temperature can control the morphology of HA crystal. Porous HA microspheres with large specific surface area were successfully prepared under the condition of ammonia diffusion at 60 鈩，

本文編號(hào)：2439016