【摘要】：目的:觀察和絡(luò)泄?jié)犷w粒對大鼠腎臟mi R-200a、mi R-29c的影響,探討和法在腎間質(zhì)纖維化的應(yīng)用。方法:選雄性SD大鼠30只,隨機(jī)分為Sham組(n=10)、UUO組(n=10)和UUO+REG組(n=10),手術(shù)采用單側(cè)輸尿管結(jié)扎術(shù)(UUO),以10%水合氯醛按0.35mg/kg腹腔注射麻醉實(shí)驗(yàn)大鼠后,取大鼠左側(cè)腹部腎區(qū)切口,逐層分離并暴露左側(cè)輸尿管,以5-0縫合線結(jié)扎腎盞和腎下極處輸尿管2道,并不離斷輸尿管,術(shù)中注意不碰傷腎包膜及周圍組織,結(jié)扎輸尿管后,腎置于原位;縫合腹膜、肌肉、皮膚。Sham組不結(jié)扎輸尿管,其余操作步驟均與上述相同。術(shù)后予Sham組和UUO組生理鹽水按10ml/kg.d-1灌胃,而UUO+REG組則給予生藥含量為1g/ml和絡(luò)泄?jié)犷w粒按10g/kg.d-1灌胃。各組于術(shù)后第7天、第14天處死5只。每個(gè)大鼠左腎臟取出后,于低溫下沿著腎臟縱切面(矢狀面)分兩半,一半腎臟組織用于RT-PCR檢測mi R-200a和mi R-29c;另一半則用于HE染色、免疫組化檢測α-SMA。結(jié)果:UUO成功建立慢性腎臟纖維大鼠模型。與Sham組相比,UUO組和UUO+REG組腎組織中α-SMA明顯增多,其隨時(shí)間的延長而增多。與Sham組相比,mi R-200a和mi R-29c在UUO組和UUO+REG組均表達(dá)下降,并隨時(shí)間的延長,表達(dá)量逐漸降低;UUO+REG組高于UUO組表達(dá)量。在第7天和第14天,與UUO組相比較,mi R-200a在Sham組表達(dá)量有明顯的統(tǒng)計(jì)學(xué)意義(P0.01);與UUO+REG組相比,與兩組亦有差異(P0.05)。在第7天,與Sham組相比,其余兩組mi R-29c表達(dá)量均較Sham組少(P0.05),UUO組與UUO+REG組在第7天無明顯差異(P0.05),但在14天UUO組表達(dá)量少于Sham組和UUO+REG組(P0.05)。結(jié)論:以和法為指導(dǎo)思想運(yùn)用和絡(luò)泄?jié)犷w粒可以增加腎臟組織mi R-200a和mi R-29c表達(dá)量,延緩腎間質(zhì)纖維化。
[Abstract]:Aim: to observe the effect of Heluoxiezhuo granule (HXG) on renal mi R-200aFMI R-29c in rats and to explore the application of Hehe method in renal interstitial fibrosis. Methods: thirty male SD rats were randomly divided into Sham group (n = 10) and UUO REG group (n = 10). The experimental rats were anesthetized with 10% chloral hydrate and 10% chloral hydrate by unilateral ureteral ligation (UUO),). The left ureter was separated and exposed layer by incision of left abdominal and renal region in rats. The ureter was ligated with 5-0 suture line and the ureter at the lower pole of the kidney was not broken, and the renal capsule and surrounding tissues were not injured. After ligation of ureter, kidney was placed in situ. Peritoneum, muscle and skin were sutured. Ureter was not ligated in Sham group. The other procedures were the same as above. After operation, Sham group and UUO group were intragastrically perfused with normal saline as 10ml/kg.d-1, while in UUO REG group, the content of crude drugs was 1g/ml and Luoxiezhuo granule was given by 10g/kg.d-1. The rats in each group were killed on the 7th day and the 14th day after operation. After each rat left kidney was removed, it was divided into two halves along the longitudinal section (sagittal plane) of the kidney at low temperature. Half of the kidney tissue was used for RT-PCR detection of mi R-200a and mi R-29c.The other half was used for HE staining and immunohistochemistry for 偽 -SMA. detection. Results: chronic renal fiber rat model was successfully established by UUO. Compared with Sham group, 偽-SMA in renal tissue of UUO group and UUO REG group increased obviously and increased with time. Compared with Sham group, the expression of mi R-200a and mi R-29c decreased in both UUO group and UUO REG group, and the expression level of; UUO REG group was lower than that of UUO group with the increase of time. On the 7th and 14th day, the expression of mi R-200a in Sham group was significantly higher than that in UUO group (P0.01), and there was also significant difference compared with UUO REG group (P0.05). On the 7th day, compared with Sham group, the expression of mi R-29c in the other two groups was lower than that in Sham group (P0.05). There was no significant difference between), UUO group and UUO REG group on the 7th day (P0.05), but on the 14th day, the expression of R-29c in UUO group was lower than that in Sham group and UUO REG group (P0.05). Conclusion: the application of Heluoxiezhuo granule can increase the expression of mi R-200a and mi R-29c in renal tissue and delay renal interstitial fibrosis.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R692

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